- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-WEE2 antibody
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DescriptionRabbit polyclonal to WEE2
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Host speciesRabbit
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Tested applicationsSuitable for: ICC/IF, IHC-Pmore details
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Species reactivityReacts with: Human
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Immunogen
antigen sequence: TKSGNHFEEP KLKDILLQIS LGLNYIHNSS MVHLDIKPSN IFICHKMQSE SSGVIEEVEN EADWFLSANV MYKIGDLGHA TSINKPKVEE GD, corresponding to amino acids 305-396 of Human WEE2.
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Positive control
- A431 cells; Human duodenum tissue.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
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Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab121943 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 1 - 4 µg/ml. using PFA/Triton X-100 Fixation/Permeabilization. | |
| IHC-P | 1/700. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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FunctionOocyte-specific protein tyrosine kinase that phosphorylates and inhibits CDK1 and acts as a key regulator of meiosis during both prophase I and metaphase II. Required to maintain meiotic arrest in oocytes during the germinal vesicle (GV) stage, a long period of quiescence at dictyate prophase I, by phosphorylating CDK1 at 'Tyr-15', leading to inhibit CDK1 activity and prevent meiotic reentry. Also required for metaphase II exit during egg activation by phosphorylating CDK1 at 'Tyr-15', to ensure exit from meiosis in oocytes and promote pronuclear formation.
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Tissue specificityExpressed in testis.
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Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsPhosphorylation leads to increase its activity. -
Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 494551 Human
- Omim: 614084 Human
- SwissProt: P0C1S8 Human
- Unigene: 657927 Human
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Alternative names
- hCG_39453 antibody
- WEE1 homolog 2 (S. pombe) antibody
- Wee1-like protein kinase 1B antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WEE2 antibody (ab121943)ab121943, at 1/700 dilution, staining WEE2 in paraffin embedded Human duodenum tissue by Immunohistochemistry. -
Immunocytochemistry/ Immunofluorescence - Anti-WEE2 antibody (ab121943)ab121943, at 4 µg/ml, staining WEE2 in PFA/Triton X-100 fixed/permeabilized A431 cells by Immunofluorescence (green).
References
ab121943 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"