Anti-WEE2 antibody (ab121943)

Reviews (1)Q&A (1)

Overview

  • Product name

    Anti-WEE2 antibody

  • Description

    Rabbit polyclonal to WEE2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    antigen sequence: TKSGNHFEEP KLKDILLQIS LGLNYIHNSS MVHLDIKPSN IFICHKMQSE SSGVIEEVEN EADWFLSANV MYKIGDLGHA TSINKPKVEE GD, corresponding to amino acids 305-396 of Human WEE2.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • A431 cells; Human duodenum tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab121943 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml. using PFA/Triton X-100 Fixation/Permeabilization.
IHC-P 1/700. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Oocyte-specific protein tyrosine kinase that phosphorylates and inhibits CDK1 and acts as a key regulator of meiosis during both prophase I and metaphase II. Required to maintain meiotic arrest in oocytes during the germinal vesicle (GV) stage, a long period of quiescence at dictyate prophase I, by phosphorylating CDK1 at 'Tyr-15', leading to inhibit CDK1 activity and prevent meiotic reentry. Also required for metaphase II exit during egg activation by phosphorylating CDK1 at 'Tyr-15', to ensure exit from meiosis in oocytes and promote pronuclear formation.

  • Tissue specificity

    Expressed in testis.

  • Sequence similarities

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.
    Contains 1 protein kinase domain.

  • Post-translational
    modifications

    Phosphorylation leads to increase its activity.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • hCG_39453 antibody
      • WEE1 homolog 2 (S. pombe) antibody
      • Wee1-like protein kinase 1B antibody
      • Wee1-like protein kinase 2 antibody
      • WEE1B antibody
      • Wee1B kinase antibody
      • Wee2 antibody
      • WEE2_HUMAN antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WEE2 antibody (ab121943)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WEE2 antibody (ab121943)
      ab121943, at 1/700 dilution, staining WEE2 in paraffin embedded Human duodenum tissue by Immunohistochemistry.
    • Immunocytochemistry/ Immunofluorescence - Anti-WEE2 antibody (ab121943)
      Immunocytochemistry/ Immunofluorescence - Anti-WEE2 antibody (ab121943)
      ab121943, at 4 µg/ml, staining WEE2 in PFA/Triton X-100 fixed/permeabilized A431 cells by Immunofluorescence (green).

    References

    ab121943 has not yet been referenced specifically in any publications.

    Publishing research using ab121943? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Immunohistochemistry (Frozen sections) abreview for Anti-WEE2 antibody

     Average
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (ovary)
    Permeabilization
    Yes - 0.3% Triton X-100, 20 min
    Specification
    ovary
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 23 2015

    Question

    Inquiry: My inquiry is related to-Anti-WEE2 antibody (ab121943) I was wondering whether it detects Wee2 from mouse. Also whether it can be used for western blotting. I am looking for an antibody to detect Wee2 in mouse oocyte by western blotting.

    Read More
    Answer

    Thank your for your inquiry.

    The immunogen of ab121943 shows only 82% homology with mouse. We generally expect a cross reactivity from 85% homology. This is a border line case and I cannot recommend using this antibody in this case since WB is also not tested.

    If there is a negative result it will not be clear if the antibody does not recognize mouse or if it is simply unsuitable for WB.

    Maybe you want to consider running a WB with human samples of doing IHC-P or ICC/IF with the mouse samples?

    I am sorry I did not have a positive answer for your on this occasion and hope this information is nevertheless helpful.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com