Overview

  • Product name
    Anti-Werner's syndrome helicase WRN antibody
    See all Werner's syndrome helicase WRN primary antibodies
  • Description
    Rabbit polyclonal to Werner's syndrome helicase WRN
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    The epitope recognized by ab17987 maps to a region between residues 400 and 450 of human Werner Syndrome Helicase.

  • Positive control
    • Whole cell lysate from 293T cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.1% Sodium azide
    Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab17987 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/25000. Detects a band of approximately 200 kDa (predicted molecular weight: 162 kDa).
IP Use at 2-5 µg/mg of lysate.

Target

  • Function
    Multifunctional enzyme that has both magnesium and ATP-dependent DNA-helicase activity and 3'->5' exonuclease activity towards double-stranded DNA with a 5'-overhang. Has no nuclease activity towards single-stranded DNA or blunt-ended double-stranded DNA. Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled replication forks or during DNA repair. Alleviates stalling of DNA polymerases at the site of DNA lesions. Important for genomic integrity. Plays a role in the formation of DNA replication focal centers; stably associates with foci elements generating binding sites for RP-A.
  • Involvement in disease
    Defects in WRN are a cause of Werner syndrome (WRN) [MIM:277700]. WRN is a rare autosomal recessive progeroid syndrome characterized by the premature onset of multiple age-related disorders, including atherosclerosis, cancer, non-insulin-dependent diabetes mellitus, ocular cataracts and osteoporosis. The major cause of death, at a median age of 47, is myocardial infarction. Currently all known WS mutations produces prematurely terminated proteins.
    Defects in WRN may be a cause of colorectal cancer (CRC) [MIM:114500].
  • Sequence similarities
    Belongs to the helicase family. RecQ subfamily.
    Contains 1 3'-5' exonuclease domain.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
    Contains 1 HRDC domain.
  • Post-translational
    modifications
    Phosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus > nucleolus. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp686C2056 antibody
    • DNA helicase antibody
    • DNA helicase, RecQ like type 3 antibody
    • Exonuclease WRN antibody
    • HGNC 12791 antibody
    • OTTHUMP00000225301 antibody
    • RecQ protein-like 2 antibody
    • RecQ-like type 3 antibody
    • RecQ3 antibody
    • RECQL2 antibody
    • RECQL3 antibody
    • Werner syndrome ATP-dependent helicase antibody
    • Werner syndrome helicase antibody
    • Werner syndrome protein antibody
    • Werner syndrome, RecQ helicase like antibody
    • WRN antibody
    • WRN_HUMAN antibody
    see all

Images

  • ab17987, at 0.1mcg/ml, staining Human WRN in whole cell lysate (100mcg) in 293T cells by Western blot. WRN was immunoprecipitated with ab17987 at 5mcg/10cm plate (+/- blocking peptide). Detection was by chemiluminescence with an exposure time of less than 10 seconds.

References

This product has been referenced in:
  • Otterlei M  et al. Werner syndrome protein participates in a complex with RAD51, RAD54, RAD54B and ATR in response to ICL-induced replication arrest. J Cell Sci 119:5137-46 (2006). WB, IP ; Human . Read more (PubMed: 17118963) »
See 1 Publication for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (4-12%)
Sample
Human Cell lysate - whole cell (lymphocytes)
Specification
lymphocytes
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Aug 05 2014

Answer

Thank you for your enquiry. As you know, the molecular weight of BSA is expected to be 67kDa, which is lower that the 75-105kDa band area that you observed. In addition, I have confirmed with the originator of this product that it does not contain BSA. I am sorry that I do not know what the extra band is, or whether it is non-specific. I am sorry I can't be of more assistance in this occasion. Should you require any further information, please do not hesitate to contact me.

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Answer

Thank you for your enquiry. I can confirm that this product is supposed to detect an approximately 200 kDa band and there are no BSA included in the product. In your previous submitted Abreview, you mentioned that you were able to obtain a specific 190kDa band and a non-specific 80kDa band. In your recent experiment, did you also managed to get a 190kDa band together with this 75~105kDa band? I am unsure what the 75~105kDa band may be but I would assume that it would a non-specific band. If you would like some technical support I enclose a link to a questionnaire which will help you put your protocol information together very easily so we can look into the details of the experiment and provide some help. https://www.abcam.com/index.html?section=western&pageconfig=technical&intAbID=17987&mode=questionaire The BL1308 and BL1309 you are referring to is actually ab17987 and ab17988, respectively. The difference between these two can be found in the immunogen sequences. The epitope recognized by ab17987 maps to a region between residues 400 and 450 if human Werner Syndrome Helicase. While, the epitope recognized by ab17988 is a region between residues 1400 and the C-terminus (residue 1432) of human Werner Syndrome Helicase. I hope the information above will be useful to you. Should you require any further information, please do not hesitate to contact me.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Jurkat cells and WSKO372 lymphoblastoid cells)
Loading amount
10 µg
Specification
Jurkat cells and WSKO372 lymphoblastoid cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Miss. mari sild

Verified customer

Submitted Sep 27 2006

Answer

Thank you for your enquiry. You ask an interesting question. I have been in discussion with the lab and they have referred me to the fact that the resolution of proteins by SDS-PAGE does not define molecular weight. It defines the relative mobility of the protein in SDS-PAGE; relative to the molecular weight standards. The molecular weight standards vary from lab to lab. One can take several different sets of molecular weight markers from the same or different sources and run them on a given gel. If done, one will see that there are discrepancies in where a given size supposedly runs on the gel. There are two sets of molecular weight markers on the market that lend themselves well to this example. One has a marker for 148 kDa, the other a marker for 180 kDa. If run side-by-side one will find that the 148 kDa marker runs higher (larger) than the 180 kDa marker. Another point is that the gel and buffer system used has a significant influence on the mobility of the molecular weight markers. The specificity of the antibodies for Werner's syndrome helicase WRN have been tested extensively. They have been shown to react against the target by IP and recognised using another of our antibodies (ab200) by subsequent western blotting. Furthermore there is no staining in human fibroblast WRN knock-out cells. The antibody works well by immunofluoresence on WRN +/+ cells and is negative on WRN -/- cells. The immunoprecipitation of this protein is at least partially blocked by the blocking peptides for both of the antibodies. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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