Product nameAnti-Werner's syndrome helicase WRN antibody
See all Werner's syndrome helicase WRN primary antibodies
DescriptionRabbit polyclonal to Werner's syndrome helicase WRN
Tested applicationsSuitable for: ELISA, ICC, IHC-P, ICC/IF, WBmore details
Species reactivityReacts with: Human
Synthetic peptide, corresponding to amino acids 1-510 of Human Werner's syndrome helicase WRN
- WRN +/+ cells
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Our Abpromise guarantee covers the use of ab200 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration. PubMed: 17118963|
|ICC||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
|WB||1/2000. Predicted molecular weight: 162 kDa.|
FunctionMultifunctional enzyme that has both magnesium and ATP-dependent DNA-helicase activity and 3'->5' exonuclease activity towards double-stranded DNA with a 5'-overhang. Has no nuclease activity towards single-stranded DNA or blunt-ended double-stranded DNA. Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled replication forks or during DNA repair. Alleviates stalling of DNA polymerases at the site of DNA lesions. Important for genomic integrity. Plays a role in the formation of DNA replication focal centers; stably associates with foci elements generating binding sites for RP-A.
Involvement in diseaseDefects in WRN are a cause of Werner syndrome (WRN) [MIM:277700]. WRN is a rare autosomal recessive progeroid syndrome characterized by the premature onset of multiple age-related disorders, including atherosclerosis, cancer, non-insulin-dependent diabetes mellitus, ocular cataracts and osteoporosis. The major cause of death, at a median age of 47, is myocardial infarction. Currently all known WS mutations produces prematurely terminated proteins.
Defects in WRN may be a cause of colorectal cancer (CRC) [MIM:114500].
Sequence similaritiesBelongs to the helicase family. RecQ subfamily.
Contains 1 3'-5' exonuclease domain.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Contains 1 HRDC domain.
modificationsPhosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationNucleus > nucleolus. Nucleus.
- Information by UniProt
- DKFZp686C2056 antibody
- DNA helicase antibody
- DNA helicase, RecQ like type 3 antibody
ab200 (1/1000) staining Werner's syndrome helicase WRN in HeLa cells (green). Cells were fixed in methanol, permeabilized with 0.5% Triton X100/ PBS and counterstained with DAPI in order to highlight the nucleus (red). Please refer to abreview for further experimental details.
ab200 (4µg/ml) staining Werner's syndrome helicase WRN in human colon using an automated system (DAKO Autostainer Plus). Using this protocol there is nuclear and some cytoplasmic staining.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH 6.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
All lanes : Anti-Werner's syndrome helicase WRN antibody (ab200) at 1/5000 dilution
Lane 1 : Human primary fibroblasts whole cell lysate
Lane 2 : Human primary fibroblasts whole cell lysate - siRNA against WRN
Lysates/proteins at 30 µg per lane.
All lanes : Alexa Fluor® 680-conjugated goat anti-rabbit IgG at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 162 kDa
Observed band size: 160 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutes
Blocked using LI-COR® Odyssey® Blocking Buffer for 45 minutes at room temperature.
Primary antibody dilution buffer: Blocker 1:1 in PBS + 0.1% Tween.
This product has been referenced in:
- Gong Y et al. PHF11 promotes DSB resection, ATR signaling, and HR. Genes Dev 31:46-58 (2017). WB ; Mouse . Read more (PubMed: 28115467) »
- Palermo V et al. CDK1 phosphorylates WRN at collapsed replication forks. Nat Commun 7:12880 (2016). Read more (PubMed: 27634057) »