Product nameAnti-Werner's syndrome helicase WRN antibody [EPR6392]
See all Werner's syndrome helicase WRN primary antibodies
DescriptionRabbit monoclonal [EPR6392] to Werner's syndrome helicase WRN
Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
Species reactivityReacts with: Human
Does not react with: Mouse, Rat
Synthetic peptide within Human Werner's syndrome helicase WRN aa 1400-1500 (C terminal). The exact sequence is proprietary.
- MOLT 4, K562 and A431 cell lysates.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab124673 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 200 kDa (predicted molecular weight: 162 kDa).|
FunctionMultifunctional enzyme that has both magnesium and ATP-dependent DNA-helicase activity and 3'->5' exonuclease activity towards double-stranded DNA with a 5'-overhang. Has no nuclease activity towards single-stranded DNA or blunt-ended double-stranded DNA. Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled replication forks or during DNA repair. Alleviates stalling of DNA polymerases at the site of DNA lesions. Important for genomic integrity. Plays a role in the formation of DNA replication focal centers; stably associates with foci elements generating binding sites for RP-A.
Involvement in diseaseDefects in WRN are a cause of Werner syndrome (WRN) [MIM:277700]. WRN is a rare autosomal recessive progeroid syndrome characterized by the premature onset of multiple age-related disorders, including atherosclerosis, cancer, non-insulin-dependent diabetes mellitus, ocular cataracts and osteoporosis. The major cause of death, at a median age of 47, is myocardial infarction. Currently all known WS mutations produces prematurely terminated proteins.
Defects in WRN may be a cause of colorectal cancer (CRC) [MIM:114500].
Sequence similaritiesBelongs to the helicase family. RecQ subfamily.
Contains 1 3'-5' exonuclease domain.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Contains 1 HRDC domain.
modificationsPhosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationNucleus > nucleolus. Nucleus.
- Information by UniProt
- DKFZp686C2056 antibody
- DNA helicase antibody
- DNA helicase, RecQ like type 3 antibody
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Werner's syndrome helicase WRN knockout HAP1 cell lysate (20 µg)
Lane 3: MOLT4 cell lysate (20 µg)
Lane 4: K562 cell lysate (20 µg)
Lanes 1 to 4: Merged signal (red and green). Green - ab124673 observed at 170 kDa. Red - loading control, ab181602, observed at 124 kDa.
ab124673 was shown to recognize Werner's syndrome helicase WRN when Werner's syndrome helicase WRN knockout samples were used, along with additional cross-reactive bands. Wild-type and Werner's syndrome helicase WRN knockout samples were subjected to SDS-PAGE. ab124673 and ab181602 (loading control to GAPDH) were both diluted at 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.
All lanes : Anti-Werner's syndrome helicase WRN antibody [EPR6392] (ab124673) at 1/1000 dilution
Lane 1 : MOLT4 cell lysates
Lane 2 : K562 cell lysates
Lane 3 : A431 cell lysates
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 162 kDa