Overview

  • Product name

    Anti-Werner's syndrome helicase WRN antibody [EPR6392]
    See all Werner's syndrome helicase WRN primary antibodies
  • Description

    Rabbit monoclonal [EPR6392] to Werner's syndrome helicase WRN
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human Werner's syndrome helicase WRN aa 1400-1500 (C terminal). The exact sequence is proprietary.

  • Positive control

    • MOLT 4, K562 and A431 cell lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR6392
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab124673 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 200 kDa (predicted molecular weight: 162 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • Function

      Multifunctional enzyme that has both magnesium and ATP-dependent DNA-helicase activity and 3'->5' exonuclease activity towards double-stranded DNA with a 5'-overhang. Has no nuclease activity towards single-stranded DNA or blunt-ended double-stranded DNA. Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled replication forks or during DNA repair. Alleviates stalling of DNA polymerases at the site of DNA lesions. Important for genomic integrity. Plays a role in the formation of DNA replication focal centers; stably associates with foci elements generating binding sites for RP-A.
    • Involvement in disease

      Defects in WRN are a cause of Werner syndrome (WRN) [MIM:277700]. WRN is a rare autosomal recessive progeroid syndrome characterized by the premature onset of multiple age-related disorders, including atherosclerosis, cancer, non-insulin-dependent diabetes mellitus, ocular cataracts and osteoporosis. The major cause of death, at a median age of 47, is myocardial infarction. Currently all known WS mutations produces prematurely terminated proteins.
      Defects in WRN may be a cause of colorectal cancer (CRC) [MIM:114500].
    • Sequence similarities

      Belongs to the helicase family. RecQ subfamily.
      Contains 1 3'-5' exonuclease domain.
      Contains 1 helicase ATP-binding domain.
      Contains 1 helicase C-terminal domain.
      Contains 1 HRDC domain.
    • Post-translational
      modifications

      Phosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization

      Nucleus > nucleolus. Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • DKFZp686C2056 antibody
      • DNA helicase antibody
      • DNA helicase, RecQ like type 3 antibody
      • Exonuclease WRN antibody
      • HGNC 12791 antibody
      • OTTHUMP00000225301 antibody
      • RecQ protein-like 2 antibody
      • RecQ-like type 3 antibody
      • RecQ3 antibody
      • RECQL2 antibody
      • RECQL3 antibody
      • Werner syndrome ATP-dependent helicase antibody
      • Werner syndrome helicase antibody
      • Werner syndrome protein antibody
      • Werner syndrome, RecQ helicase like antibody
      • WRN antibody
      • WRN_HUMAN antibody
      see all

    Images

    • Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: Werner's syndrome helicase WRN knockout HAP1 cell lysate (20 µg)
      Lane 3: MOLT4 cell lysate (20 µg)
      Lane 4: K562 cell lysate (20 µg)
      Lanes 1 to 4: Merged signal (red and green). Green - ab124673 observed at 170 kDa. Red - loading control, ab181602, observed at 124 kDa.
      ab124673 was shown to recognize Werner's syndrome helicase WRN when Werner's syndrome helicase WRN knockout samples were used, along with additional cross-reactive bands. Wild-type and Werner's syndrome helicase WRN knockout samples were subjected to SDS-PAGE. ab124673 and ab181602 (loading control to GAPDH) were both diluted at 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

    • All lanes : Anti-Werner's syndrome helicase WRN antibody [EPR6392] (ab124673) at 1/1000 dilution

      Lane 1 : MOLT4 cell lysates
      Lane 2 : K562 cell lysates
      Lane 3 : A431 cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size: 162 kDa

    References

    This product has been referenced in:

    See 1 Publication for this product

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HAP1)
    Gel Running Conditions
    Reduced Denaturing (6%)
    Loading amount
    20 µg
    Treatment
    CPT 1uM 2hr
    Specification
    HAP1
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Jan 23 2019

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