Anti-West Nile Virus M glycoprotein antibody (ab22070)
Key features and details
- Rabbit polyclonal to West Nile Virus M glycoprotein
- Suitable for: IHC-P, WB
- Reacts with: West Nile virus
- Isotype: IgG
Overview
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Product name
Anti-West Nile Virus M glycoprotein antibody -
Description
Rabbit polyclonal to West Nile Virus M glycoprotein -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: West Nile virus -
Immunogen
Synthetic peptide corresponding to West Nile Virus M glycoprotein aa 8-27.
Sequence:GESTLANKKGAWLDSTKATR
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab22070 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (2) |
Use a concentration of 1 µg/ml.
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WB | (1) |
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 8 kDa.
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Notes |
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IHC-P
Use a concentration of 1 µg/ml. |
WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 8 kDa. |
Target
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Relevance
West Nile (WN), the most widespread among flaviviruses, was first isolated from the serum of a febrile woman in 1937 in the West Nile district of Uganda. West Nile virus was first detected in North America in 1999 and has subsequently spread throughout the United States and Canada and into Mexico and the Caribbean. In Africa, southern Europe, western Asia, and the United States, WNV has been isolated from mosquitoes of more than 40 species. In the United States, Canada, and Israel, WNV is responsible for significant avian mortality. -
Database links
- Entrez Gene: 912267 West Nile virus
- SwissProt: P06935 West Nile virus
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Alternative names
- Envelope protein M antibody
- Matrix protein antibody
- West Nile virus matrix protein antibody
- WNV matrix protein antibody
Images
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IHC analysis of WNV-Envelope protein in bird liver using ab22070 at 1ug/ ml incubated for 30 minutes. A secondary conjugated to biotin was applied followed with streptavidin-AKP. The substrate was Fast Red chromagen. Image courtesy of Angela Ellis, University of Georgia College of Veterinary Medicine
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Western blot analysis of West Nile Virus glycoprotein M in (A) untransfected and (B) transfected cells using ab22070 at 1 ug/ml.
Western blot analysis of West Nile Virus glycoprotein M in (A) untransfected and (B) transfected cells using ab22070 at 1 ug/ml.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab22070 has been referenced in 6 publications.
- Zaccaria G et al. The envelope protein of Usutu virus attenuates West Nile virus virulence in immunocompetent mice. Vet Microbiol 263:109262 (2021). PubMed: 34715462
- Spedicato M et al. Transplacental transmission of the Italian Bluetongue virus serotype 2 in sheep Vet Ital 55:131-141 (2019). PubMed: 31274174
- Mukherjee S et al. Mechanism and significance of cell type-dependent neutralization of flaviviruses. J Virol 88:7210-20 (2014). PubMed: 24741083
- Velado Fernández I et al. Development of a novel protocol for generating flavivirus reporter particles. J Virol Methods 208:96-101 (2014). PubMed: 25116200
- Savini G et al. West Nile virus lineage 2 in Sardinian wild birds in 2012: a further threat to public health. Epidemiol Infect 141:2313-6 (2013). PubMed: 23343580
- Schepp-Berglind J et al. Complex adenovirus-mediated expression of West Nile virus C, PreM, E, and NS1 proteins induces both humoral and cellular immune responses. Clin Vaccine Immunol 14:1117-26 (2007). ICC/IF . PubMed: 17634508