Key features and details
- Rabbit polyclonal to West Nile Virus M glycoprotein
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: West Nile virus
- Isotype: IgG
Product nameAnti-West Nile Virus M glycoprotein antibody
DescriptionRabbit polyclonal to West Nile Virus M glycoprotein
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: West Nile virus
- West Nile virus infected cell lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab22070 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 1 µg/ml.|
|WB||Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 8 kDa.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 17634508|
RelevanceWest Nile (WN), the most widespread among flaviviruses, was first isolated from the serum of a febrile woman in 1937 in the West Nile district of Uganda. West Nile virus was first detected in North America in 1999 and has subsequently spread throughout the United States and Canada and into Mexico and the Caribbean. In Africa, southern Europe, western Asia, and the United States, WNV has been isolated from mosquitoes of more than 40 species. In the United States, Canada, and Israel, WNV is responsible for significant avian mortality.
- Envelope protein M antibody
- Matrix protein antibody
- West Nile virus matrix protein antibody
- WNV matrix protein antibody
IHC analysis of WNV-Envelope protein in bird liver using ab22070 at 1ug/ ml incubated for 30 minutes. A secondary conjugated to biotin was applied followed with streptavidin-AKP. The substrate was Fast Red chromagen. Image courtesy of Angela Ellis, University of Georgia College of Veterinary Medicine
Western blot analysis of West Nile Virus glycoprotein M in (A) untransfected and (B) transfected cells using ab22070 at 1 ug/ml.Western blot analysis of West Nile Virus glycoprotein M in (A) untransfected and (B) transfected cells using ab22070 at 1 ug/ml.
ab22070 has been referenced in 4 publications.
- Mukherjee S et al. Mechanism and significance of cell type-dependent neutralization of flaviviruses. J Virol 88:7210-20 (2014). PubMed: 24741083
- Velado Fernández I et al. Development of a novel protocol for generating flavivirus reporter particles. J Virol Methods 208:96-101 (2014). PubMed: 25116200
- Savini G et al. West Nile virus lineage 2 in Sardinian wild birds in 2012: a further threat to public health. Epidemiol Infect 141:2313-6 (2013). PubMed: 23343580
- Schepp-Berglind J et al. Complex adenovirus-mediated expression of West Nile virus C, PreM, E, and NS1 proteins induces both humoral and cellular immune responses. Clin Vaccine Immunol 14:1117-26 (2007). ICC/IF . PubMed: 17634508