Key features and details
- Rabbit polyclonal to West Nile Virus preM
- Suitable for: ELISA, ICC/IF, Functional Studies
- Reacts with: West Nile virus
- Isotype: IgG
Product nameAnti-West Nile Virus preM antibody
DescriptionRabbit polyclonal to West Nile Virus preM
Tested applicationsSuitable for: ELISA, ICC/IF, Functional Studiesmore details
Species reactivityReacts with: West Nile virus
Synthetic peptide corresponding to 15 amino acids near the middle of the West Nile Virus Matrix precursor protein.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab25888 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent dilution. This polyclonal antibody can be used for the detection of the WNV Matrix precursor protein in ELISA. It will detect 10 ng of free peptide at 1 µg/ml.|
|ICC/IF||Use at an assay dependent dilution. PubMed: 17634508|
|Functional Studies||Use at an assay dependent concentration. PubMed: 21697345|
RelevanceWest Nile Virus is part of the flavivirus family, many of which are capable of causing serious illness in humans. The ssRNA + strand virus is enveloped and spherical with a diameter of 40-50 nm, similar to all flaviviruses. The virion of this virus is a nucleocapsid covered by a lipoprotein envelope. The envelope contains two proteins: the protein M and glycoprotein E. The nucleocapsid is a complex of protein C and mRNA. In immature particles, there are 60 icosaedrally organized trimeric spikes on the surface. Each spike consists of three heterodimers of envelope protein M precursor (prM) and envelope protein E. Inside cells, viral particles will contain a preM particle and E in a complex that matures through proteolytic cleavage and processing in the Golgi apparatus. WNV has three different effects on humans, the first is asymptomatic infection; the second is a mild febrile syndrome termed West Nile Fever; the third is a neuroinvasive disease termed West Nile meningitis or encephalitis. In infected individuals the ratio between the three states is roughly 110:30:1. The main route of human infection is through the bite of an infected mosquito.
- membrane glycoprotein M antibody
- membrane glycoprotein precursor prM antibody
- pre M protein antibody
- West Nile virus antibody
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab25888 has been referenced in 3 publications.
- Hirota J et al. Development of monoclonal antibodies to West Nile virus and their application in immunohistochemistry. Clin Vaccine Immunol : (2012). PubMed: 22993408
- Colpitts TM et al. prM-antibody renders immature West Nile virus infectious in vivo. J Gen Virol 92:2281-5 (2011). Functional Studies . PubMed: 21697345
- Schepp-Berglind J et al. Complex adenovirus-mediated expression of West Nile virus C, PreM, E, and NS1 proteins induces both humoral and cellular immune responses. Clin Vaccine Immunol 14:1117-26 (2007). ICC/IF . PubMed: 17634508