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  1. Link

    western-blot-marker-pen-ab166858.pdf

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Kits/ Lysates/ Other Tools and Reagents Western Blot Tools and Reagents Accessory reagents
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Western Blot Marker Pen (ab166858)

  • Datasheet
  • SDS
Reviews (1)Q&A (2)

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Western blot - Western Blot Marker Pen (ab166858)
  • Western blot - Western Blot Marker Pen (ab166858)

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Overview

  • Product name

    Western Blot Marker Pen
  • Tested applications

    Suitable for: WBmore details
  • General notes

    Western Blot Marker Pen ab166858 is used to annotate the blot and/or to mark the positions of (pre-stained) molecular weight standard markers on a PVDF or nitrocellulose membrane during western blotting. The pen is used after the transfer of proteins from the gel to the membrane. The marks made with the pen can be visualized by chemiluminescence after development of the blot, as well as directly in normal light when using colorimetric development or when marking the membrane.

    Without the pen, it is often necessary to align the western blot result with the molecular weight markers on the original membrane blot to estimate the molecular weight of the proteins of interest. Using the marks made by the pen reduces the errors made during the alignment process.

    This product was previously called Optiblot Luminol Membrane Pen.

     

Properties

  • Form

    No information available
  • Storage instructions

    Store at +4°C. Do not freeze.
  • Storage buffer

    Constituents: 10% Glycerol (glycerin, glycerine), 1% Coomassie Brilliant Blue
  • Concentration information loading...
  • Research areas

    • Kits/ Lysates/ Other
    • Tools and Reagents
    • Western Blot Tools and Reagents
    • Accessory reagents

Associated products

  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)
    • InstantBlue® Coomassie Protein Stain (ISB1L) (ab119211)
    • ECL Substrate Kit (High Sensitivity) (ab133406)
    • Nitrocellulose Transfer Membrane- 0.22 µm (ab133413)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab166858 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use at an assay dependent concentration.
Notes
WB
Use at an assay dependent concentration.

Images

  • Western blot - Western Blot Marker Pen (ab166858)
    Western blot - Western Blot Marker Pen (ab166858)
  • Western blot - Western Blot Marker Pen (ab166858)
    Western blot - Western Blot Marker Pen (ab166858)

    During the colorimetric or chemiluminenscent development step, the bands of molecular weight standard marker can be visualized and recorded for further analysis.

    Susceptible protein bands are easily located from the WB result. (red arrow indicated). Additionally, in WB application, the Optiblot Luminol Membrane Pen can be used as personal noting or marking as positive control.

Protocols

  • Protocol Booklet

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab166858? Please let us know so that we can cite the reference in this datasheet.

ab166858 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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1-3 of 3 Abreviews or Q&A

nice pen

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Abreviews
The pen works well and is easy to handle.
The signal is strong and precise. We use the pen to label the bands of pre-stained molecular weight markers to make them visible in chemiluminescence.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Mar 06 2018

Question

My proteins of interest are not very abundant in my samples and so I expose my films sometimes for a very long time (5 min minimum up to around 30 min). If I used this pen, would the areas marked with this pen (such as molecular weight marker) become overexposed, and thus potentially interfere with neighbouring wells?

Read More

Abcam community

Verified customer

Asked on Jun 03 2013

Answer


When the membrane needs to be exposed for a long time, we suggest drawing a weaker band as far as possible from the wells on the membrane with the luminol pen in order to avoid interference.

Read More

Abcam Scientific Support

Answered on Jun 03 2013

Question

How many protein markers can be stained with LuminolPen roughly (100-1000??)?

Read More

Abcam community

Verified customer

Asked on Sep 09 2014

Answer

The LuminolPen can provide 1,000 membranes drawing.

Read More

Abcam Scientific Support

Answered on Sep 09 2014

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