Western Blot Marker Pen (ab166858)

Abreviews (1)Q&A (2)

Overview

  • Product name
    Western Blot Marker Pen
  • Tested applications
    Suitable for: WBmore details
  • General notes

    Western Blot Marker Pen ab166858 is used to annotate the blot and/or to mark the positions of (pre-stained) molecular weight standard markers on a PVDF or nitrocellulose membrane during western blotting. The pen is used after the transfer of proteins from the gel to the membrane. The marks made with the pen can be visualized by chemiluminescence after development of the blot, as well as directly in normal light when using colorimetric development or when marking the membrane.

    Without the pen, it is often necessary to align the western blot result with the molecular weight markers on the original membrane blot to estimate the molecular weight of the proteins of interest. Using the marks made by the pen reduces the errors made during the alignment process.

    This product was previously called Optiblot Luminol Membrane Pen.

     

Properties

Applications

Our Abpromise guarantee covers the use of ab166858 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.

Images

  • - Optiblot Luminol Membrane Pen (ab166858)
    - Optiblot Luminol Membrane Pen (ab166858)
  • Western blot - Optiblot Luminol Membrane Pen (ab166858)
    Western blot - Optiblot Luminol Membrane Pen (ab166858)

    During the colorimetric or chemiluminenscent development step, the bands of molecular weight standard marker can be visualized and recorded for further analysis.

    Susceptible protein bands are easily located from the WB result. (red arrow indicated). Additionally, in WB application, the Optiblot Luminol Membrane Pen can be used as personal noting or marking as positive control.

Protocols

References

ab166858 has not yet been referenced specifically in any publications.

Publishing research using ab166858? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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3 reviews or Q&A

10
Votes: Highest First

nice pen

 Excellent Excellent 5/5 (Ease of Use)
Abreviews
The pen works well and is easy to handle.
The signal is strong and precise. We use the pen to label the bands of pre-stained molecular weight markers to make them visible in chemiluminescence.
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Verified customer

Submitted Mar 06 2018

My proteins of interest are not very abundant in my samples and so I expose my films sometimes for a very long time (5 min minimum up to around 30 min). If I used this pen, would the areas marked with this pen (such as molecular weight marker) become o...

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When the membrane needs to be exposed for a long time, we suggest drawing a weaker band as far as possible from the wells on the membrane with the luminol pen in order to avoid interference.

How many protein markers can be stained with LuminolPen roughly (100-1000??)?

The LuminolPen can provide 1,000 membranes drawing.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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