Overview

  • Product name

    Anti-WFDC1/PS20 antibody
  • Description

    Rabbit polyclonal to WFDC1/PS20
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human WFDC1/PS20 aa 148-177 (C terminal) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Database link: Q9HC57

  • Positive control

    • IHC-P: human kidney and liver tissues. WB: HeLa cell lysate. Flow Cyt: HeLa cells.
  • General notes

     This product was previously labelled as WFDC1

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab230326 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100.
WB 1/1000. Predicted molecular weight: 24 kDa.
Flow Cyt 1/10 - 1/50.

Target

  • Function

    Has growth inhibitory activity.
  • Sequence similarities

    Contains 1 WAP domain.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • Prostate stromal protein ps20 antibody
    • PS20 antibody
    • ps20 growth inhibitor antibody
    • WAP four disulfide core domain 1 antibody
    • WAP four disulfide core domain 1 homolog antibody
    • WAP four-disulfide core domain protein 1 antibody
    • Wfdc1 antibody
    • WFDC1_HUMAN antibody
    see all

Images

  • Anti-WFDC1/PS20 antibody (ab230326) at 1/1000 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 35 µg

    Predicted band size: 24 kDa

  • Formalin-fixed, paraffin-embedded human liver tissue stained for WFDC1/PS20 using ab230326 at 1/100 dilution in immunohistochemical analysis.

  • Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling WFDC1/PS20 with ab230326 at 1/10 dilution (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

  • Formalin-fixed, paraffin-embedded human kidney tissue stained for WFDC1/PS20 using ab230326 at 1/100 dilution in immunohistochemical analysis.

References

ab230326 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Testis, postnatal day 4)
Permeabilization
Yes - 0.1% Triton X-100 in PBS
Specification
Testis, postnatal day 4
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Paraformaldehyde

Mr. Bryan Niedenberger

Verified customer

Submitted Jul 23 2019

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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