Immunohistochemistry (Frozen sections) abreview for Anti-WFDC1/PS20 antibody

Below Average
Abreviews
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Testis, postnatal day 4)
Permeabilization
Yes - 0.1% Triton X-100 in PBS
Specification
Testis, postnatal day 4
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Paraformaldehyde

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Temperature: 20°C
Dilution
1/250

Secondary antibody

Dilution
1/500

Additional data

Additional Notes
Staining appears to be brightest in the nuclei of spermatogonia, however background was very high throughout the testis cords.
Abcam response
Thank you for sharing this data with us. Previously ab230326 has not been tested on mouse, or in IHC-Fr. Base on the data shown here this antibody either does not work on mouse, in IHC-Fr, or both, under these experimental conditions. We would welcome feedback from other researchers currently using this antibody.

Mr. Bryan Niedenberger

Verified customer

Submitted Jul 23 2019

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