Overview

  • Product name

  • Description

    Rabbit polyclonal to WFS1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide within Human WFS1 aa 183-232. The exact sequence is proprietary.
    Database link: O76024

  • Positive control

    • IHC-P: Human testis tissue. WB: Mouse brain extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab230512 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/500 - 1/1000. Predicted molecular weight: 100 kDa.

Target

  • Relevance

    WFS1 is a novel component of Wolfram syndrome, a rare form of juvenile diabetes. WFS1 plays an important role in maintaining homeostasis of the endoplasmic reticulum (ER) in the pancreas. It is normally up-regulated during insulin secretion, whereas inactivation of the protein can cause ER stress. Chronic ER stress is a major involvement in Wolfram syndrome.
  • Cellular localization

    Endoplasmic reticulum; endoplasmic reticulum membrane; multipass membrane protein
  • Database links

  • Alternative names

    • CTRCT41 antibody
    • DFNA14 antibody
    • DFNA38 antibody
    • DFNA6 antibody
    • DIDMOAD antibody
    • WFRS antibody
    • WFS antibody
    • WFSL antibody
    • Wolfram syndrome 1 (wolframin) antibody
    • Wolfram syndrome antibody
    • WOLFRAMIN antibody
    • wolframin ER transmembrane glycoprotein antibody
    see all

Images

  • Formalin-fixed, paraffin-embedded human testis tissue stained for WFS1 using ab230512 at 1:200 dilution in immunohistochemical analysis.

  • Anti-WFS1 antibody (ab230512) at 1/500 dilution + Mouse brain extract

    Predicted band size: 100 kDa

References

This product has been referenced in:

See 1 Publication for this product

Customer reviews and Q&As

Filter by Application

Filter by Species

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Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rhesus monkey Tissue sections (Basal Ganglia (Brain))
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: High pH followed by Low pH citrate buffer
Permeabilization
Yes - 0.1% Triton-x-100
Specification
Basal Ganglia (Brain)
Blocking step
Serum as blocking agent for 45 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Z-FIX

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Verified customer

Submitted Apr 22 2019

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