Overview

  • Product name
    Anti-WHAMM antibody
    See all WHAMM primary antibodies
  • Description
    Rabbit polyclonal to WHAMM
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: RALSSSSQAA THQNLGFRAP VKDDQPRPLV CESPAERPRD SLESFSCPGS MDEVLASLRH GRAPLRKVEV PAVRPPHASI NEHILAAIR, corresponding to internal sequence amino acids 660-748 of Human WHAMM.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • Human hippocampus tissue; A431 cells; Human liver, Human tonsil, RT 4 and U 251 MG lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab122572 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100
WB 1/250 - 1/500.

Target

  • Function
    Acts as a nucleation-promoting factor (NPF) that stimulates Arp2/3-mediated actin polymerization both at the Golgi apparatus and along tubular membranes. Involved as a regulator of Golgi positioning and morphology. Participates in vesicle transport between the reticulum endoplasmic and the Golgi complex.
  • Tissue specificity
    Expressed in brain, lung, heart, colon and kidney (at protein level).
  • Sequence similarities
    Contains 2 WH2 domains.
  • Domain
    The N-terminal region associates with membranes, the coiled-coil region binds to microtubules and the WH2 domains promotes actin nucleation.
  • Cellular localization
    Cytoplasm. Endoplasmic reticulum-Golgi intermediate compartment. Cytoplasmic vesicle membrane. Golgi apparatus > cis-Golgi network. Localized to a perinuclear compartment near the microtubule-organizing center (MTOC). Also detected on tubulo-vesicular structures in the cell periphery that frequently localized along microtubules.
  • Information by UniProt
  • Database links
    • Alternative names
      • KIAA1971 antibody
      • membranes and microtubules antibody
      • WAS protein homolog associated with actin, golgi membranes and microtubules antibody
      • WAS protein homology region 2 domain containing 1 antibody
      • WAS protein homology region 2 domain-containing protein 1 antibody
      • WASP homolog-associated protein with actin antibody
      • WASP homolog-associated protein with actin, membranes and microtubules antibody
      • WH2 domain-containing protein 1 antibody
      • WHAMM antibody
      • WHAMM_HUMAN antibody
      • WHDC1 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WHAMM antibody (ab122572)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WHAMM antibody (ab122572)
      ab122572, at 1/200 dilution, staining WHAMM in paraffin-embedded Human hippocampus tissue by Immunohistochemistry.
    • Immunocytochemistry/ Immunofluorescence - Anti-WHAMM antibody (ab122572)
      Immunocytochemistry/ Immunofluorescence - Anti-WHAMM antibody (ab122572)
      ab122572, at 4 µg/ml, staining WHAMM in PFA/Triton X-100 fixed/permeabilized A431 cells by Immunofluorescence (green).
    • Western blot - Anti-WHAMM antibody (ab122572)
      Western blot - Anti-WHAMM antibody (ab122572)
      All lanes : Anti-WHAMM antibody (ab122572) at 1/250 dilution

      Lane 1 : RT 4 cell lysate
      Lane 2 : U 251 MG cell lysate
      Lane 3 : Human plasma
      Lane 4 : Human liver lysate
      Lane 5 : Human tonsil lysate

      Developed using the ECL technique.

    References

    ab122572 has not yet been referenced specifically in any publications.

    Publishing research using ab122572? Please let us know so that we can cite the reference in this datasheet.

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