Anti-WHAMM antibody (ab122572)
Key features and details
- Rabbit polyclonal to WHAMM
- Suitable for: IHC-P, WB, ICC
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-WHAMM antibody -
Description
Rabbit polyclonal to WHAMM -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICCmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen sequence: RALSSSSQAA THQNLGFRAP VKDDQPRPLV CESPAERPRD SLESFSCPGS MDEVLASLRH GRAPLRKVEV PAVRPPHASI NEHILAAIR, corresponding to internal sequence amino acids 660-748 of Human WHAMM.
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Positive control
- IHC-P: Human endometrium, placenta, and lymph node tissues; ICC: A431 cells; WB: U-138 MG cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store undiluted. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab122572 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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ICC |
Use a concentration of 0.25 - 2 µg/ml.
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Notes |
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IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
ICC
Use a concentration of 0.25 - 2 µg/ml. |
Target
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Function
Acts as a nucleation-promoting factor (NPF) that stimulates Arp2/3-mediated actin polymerization both at the Golgi apparatus and along tubular membranes. Involved as a regulator of Golgi positioning and morphology. Participates in vesicle transport between the reticulum endoplasmic and the Golgi complex. -
Tissue specificity
Expressed in brain, lung, heart, colon and kidney (at protein level). -
Sequence similarities
Contains 2 WH2 domains. -
Domain
The N-terminal region associates with membranes, the coiled-coil region binds to microtubules and the WH2 domains promotes actin nucleation. -
Cellular localization
Cytoplasm. Endoplasmic reticulum-Golgi intermediate compartment. Cytoplasmic vesicle membrane. Golgi apparatus > cis-Golgi network. Localized to a perinuclear compartment near the microtubule-organizing center (MTOC). Also detected on tubulo-vesicular structures in the cell periphery that frequently localized along microtubules. - Information by UniProt
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Database links
- Entrez Gene: 123720 Human
- Omim: 612393 Human
- SwissProt: Q8TF30 Human
- Unigene: 377360 Human
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Alternative names
- KIAA1971 antibody
- membranes and microtubules antibody
- WAS protein homolog associated with actin, golgi membranes and microtubules antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human endometrium tissue labelling WHAMM with ab122572 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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ab122572, at 4 µg/ml, staining WHAMM in PFA/Triton X-100 fixed/permeabilized A431 cells by Immunofluorescence (green).
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Anti-WHAMM antibody (ab122572) at 0.4 µg/ml + U-138 MG (human brain glioblastoma cell line) cell lysate
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placenta tissue labelling WHAMM with ab122572 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lymph node tissue labelling WHAMM with ab122572 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab122572 has been referenced in 2 publications.
- Fung TS et al. Parallel kinase pathways stimulate actin polymerization at depolarized mitochondria. Curr Biol 32:1577-1592.e8 (2022). PubMed: 35290799
- Moore AS et al. Actin cables and comet tails organize mitochondrial networks in mitosis. Nature 591:659-664 (2021). PubMed: 33658713