Anti-WHAMM antibody (ab122572)
Key features and details
- Rabbit polyclonal to WHAMM
- Suitable for: IHC-P, WB, ICC
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-WHAMM antibody
See all WHAMM primary antibodies -
Description
Rabbit polyclonal to WHAMM -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC HumanIHC-P HumanWB Human
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Immunogen
antigen sequence: RALSSSSQAA THQNLGFRAP VKDDQPRPLV CESPAERPRD SLESFSCPGS MDEVLASLRH GRAPLRKVEV PAVRPPHASI NEHILAAIR, corresponding to internal sequence amino acids 660-748 of Human WHAMM.
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Positive control
- IHC-P: Human endometrium, placenta, and lymph node tissues; ICC: A431 cells; WB: U-138 MG cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store undiluted. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab122572 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ICC |
Human
|
| IHC-P |
Human
|
| WB |
Human
|
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
| WB |
Use a concentration of 0.04 - 0.4 µg/ml.
|
|
| ICC |
Use a concentration of 0.25 - 2 µg/ml.
|
| Notes |
|---|
|
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
|
ICC
Use a concentration of 0.25 - 2 µg/ml. |
Target
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Function
Acts as a nucleation-promoting factor (NPF) that stimulates Arp2/3-mediated actin polymerization both at the Golgi apparatus and along tubular membranes. Involved as a regulator of Golgi positioning and morphology. Participates in vesicle transport between the reticulum endoplasmic and the Golgi complex. -
Tissue specificity
Expressed in brain, lung, heart, colon and kidney (at protein level). -
Sequence similarities
Contains 2 WH2 domains. -
Domain
The N-terminal region associates with membranes, the coiled-coil region binds to microtubules and the WH2 domains promotes actin nucleation. -
Cellular localization
Cytoplasm. Endoplasmic reticulum-Golgi intermediate compartment. Cytoplasmic vesicle membrane. Golgi apparatus > cis-Golgi network. Localized to a perinuclear compartment near the microtubule-organizing center (MTOC). Also detected on tubulo-vesicular structures in the cell periphery that frequently localized along microtubules. - Information by UniProt
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Database links
- Entrez Gene: 123720 Human
- Omim: 612393 Human
- SwissProt: Q8TF30 Human
- Unigene: 377360 Human
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Alternative names
- KIAA1971 antibody
- membranes and microtubules antibody
- WAS protein homolog associated with actin, golgi membranes and microtubules antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WHAMM antibody (ab122572)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human endometrium tissue labelling WHAMM with ab122572 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunocytochemistry - Anti-WHAMM antibody (ab122572)ab122572, at 4 µg/ml, staining WHAMM in PFA/Triton X-100 fixed/permeabilized A431 cells by Immunofluorescence (green). -
Western blot - Anti-WHAMM antibody (ab122572)Anti-WHAMM antibody (ab122572) at 0.4 µg/ml + U-138 MG (human brain glioblastoma cell line) cell lysate -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WHAMM antibody (ab122572)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placenta tissue labelling WHAMM with ab122572 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WHAMM antibody (ab122572)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lymph node tissue labelling WHAMM with ab122572 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
References (0)
ab122572 has not yet been referenced specifically in any publications.
