Anti-WHAMM antibody (ab122572)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-WHAMM antibody
See all WHAMM primary antibodies -
Description
Rabbit polyclonal to WHAMM -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen sequence: RALSSSSQAA THQNLGFRAP VKDDQPRPLV CESPAERPRD SLESFSCPGS MDEVLASLRH GRAPLRKVEV PAVRPPHASI NEHILAAIR, corresponding to internal sequence amino acids 660-748 of Human WHAMM.
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Positive control
- Human hippocampus tissue; A431 cells; Human liver, Human tonsil, RT 4 and U 251 MG lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store undiluted. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab122572 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC/IF | Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100 | |
| WB | 1/250 - 1/500. |
Target
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Function
Acts as a nucleation-promoting factor (NPF) that stimulates Arp2/3-mediated actin polymerization both at the Golgi apparatus and along tubular membranes. Involved as a regulator of Golgi positioning and morphology. Participates in vesicle transport between the reticulum endoplasmic and the Golgi complex. -
Tissue specificity
Expressed in brain, lung, heart, colon and kidney (at protein level). -
Sequence similarities
Contains 2 WH2 domains. -
Domain
The N-terminal region associates with membranes, the coiled-coil region binds to microtubules and the WH2 domains promotes actin nucleation. -
Cellular localization
Cytoplasm. Endoplasmic reticulum-Golgi intermediate compartment. Cytoplasmic vesicle membrane. Golgi apparatus > cis-Golgi network. Localized to a perinuclear compartment near the microtubule-organizing center (MTOC). Also detected on tubulo-vesicular structures in the cell periphery that frequently localized along microtubules. - Information by UniProt
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Database links
- Entrez Gene: 123720 Human
- Omim: 612393 Human
- SwissProt: Q8TF30 Human
- Unigene: 377360 Human
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Alternative names
- KIAA1971 antibody
- membranes and microtubules antibody
- WAS protein homolog associated with actin, golgi membranes and microtubules antibody
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Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WHAMM antibody (ab122572)ab122572, at 1/200 dilution, staining WHAMM in paraffin-embedded Human hippocampus tissue by Immunohistochemistry. -
Immunocytochemistry/ Immunofluorescence - Anti-WHAMM antibody (ab122572)ab122572, at 4 µg/ml, staining WHAMM in PFA/Triton X-100 fixed/permeabilized A431 cells by Immunofluorescence (green). -
Western blot - Anti-WHAMM antibody (ab122572)All lanes : Anti-WHAMM antibody (ab122572) at 1/250 dilution
Lane 1 : RT 4 cell lysate
Lane 2 : U 251 MG cell lysate
Lane 3 : Human plasma
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate
Developed using the ECL technique.
Protocols
Datasheets and documents
References
ab122572 has not yet been referenced specifically in any publications.
