Overview

  • Product name

    Anti-WHAMM antibody - C-terminal
    See all WHAMM primary antibodies
  • Description

    Rabbit polyclonal to WHAMM - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rat, Horse, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within the C terminal amino acids 599-648 (SEAGNVKSPK CQNCHGNIPV QVFVPVGDQT HSKSSEELSL PPPPPPPPPP) of the Human WHAMM protein (NP_001073904).

  • Positive control

    • WB: Human Placenta lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab135414 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 91 kDa.

Target

  • Function

    Acts as a nucleation-promoting factor (NPF) that stimulates Arp2/3-mediated actin polymerization both at the Golgi apparatus and along tubular membranes. Involved as a regulator of Golgi positioning and morphology. Participates in vesicle transport between the reticulum endoplasmic and the Golgi complex.
  • Tissue specificity

    Expressed in brain, lung, heart, colon and kidney (at protein level).
  • Sequence similarities

    Contains 2 WH2 domains.
  • Domain

    The N-terminal region associates with membranes, the coiled-coil region binds to microtubules and the WH2 domains promotes actin nucleation.
  • Cellular localization

    Cytoplasm. Endoplasmic reticulum-Golgi intermediate compartment. Cytoplasmic vesicle membrane. Golgi apparatus > cis-Golgi network. Localized to a perinuclear compartment near the microtubule-organizing center (MTOC). Also detected on tubulo-vesicular structures in the cell periphery that frequently localized along microtubules.
  • Information by UniProt
  • Database links

  • Alternative names

    • KIAA1971 antibody
    • membranes and microtubules antibody
    • WAS protein homolog associated with actin, golgi membranes and microtubules antibody
    • WAS protein homology region 2 domain containing 1 antibody
    • WAS protein homology region 2 domain-containing protein 1 antibody
    • WASP homolog-associated protein with actin antibody
    • WASP homolog-associated protein with actin, membranes and microtubules antibody
    • WH2 domain-containing protein 1 antibody
    • WHAMM antibody
    • WHAMM_HUMAN antibody
    • WHDC1 antibody
    see all

Images

  • Anti-WHAMM antibody - C-terminal (ab135414) at 1 µg/ml + Placenta lysate at 10 µg

    Predicted band size: 91 kDa



    Blocking with 5% milk.

References

ab135414 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for contacting us.

As indicated on the product datasheet, https://www.abcam.com/ab135414, the recommended concentration for Western Blot is 1µg/ml and the stock concentration is 1mg/ml.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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