Anti-White spot syndrome virus VP28 antibody (ab26935)
- Datasheet
- References
- Protocols
Overview
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Product nameAnti-White spot syndrome virus VP28 antibody
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DescriptionRabbit polyclonal to White spot syndrome virus VP28
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Host speciesRabbit
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Tested applicationsSuitable for: ELISA, WBmore details
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Species reactivityReacts with Shrimp WSSV. Not yet tested in other species.
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Immunogen
Synthetic peptide corresponding to White spot syndrome virus VP28 aa 69-149. Synthetic peptide - corresponding to the VP 28 central domain (amino acids 69-148) of shrimp White spot syndrome virus.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferConstituent: Whole serum
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PurityWhole antiserum
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab26935 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | 1/5000. | |
| WB | 1/400 - 1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 23 kDa). Use at 1/400 for for tissue extracts/cell lysates, and at 1/1000 for recombinant protein. |
Target
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RelevanceWhite spot syndrome virus (WSSV) occurs worldwide and causes high mortality and considerable economic damage to the shrimp farming industry. The VP28 was one of the envelope proteins of WSSV. Almost all shrimps infected with WSSV in ponds die within 3 to 4 days after the first dead shrimp was observed with gross lesions ranging from abnormal red body discoloration to white spots in the cuticle.
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Alternative names
- Shrimp white spot syndrome virus VP 28 antibody
- Shrimp white spot syndrome virus VP28 antibody
- VP 28 antibody
see all
Images
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Western blot - Anti-White spot syndrome virus VP28 antibody (ab26935)All lanes : Anti-White spot syndrome virus VP28 antibody (ab26935) at 1/1000 dilution
Lane 1 : Lane (A M1) Control: E.Coli total cell lysate before IPTG induction, Pre bleed
Lane 2 : Lane (A M2) Recombinant VP28 protein expression after IPTG induction, Pre Bleed
Lane 3 : Lane (B M1) Control: E.Coli total cell lysate before IPTG induction, Post immunization.
Lane 4 : Lane (B M2) Recombinant VP28 protein expression after IPTG induction, Post immunization.
Secondary
All lanes : anti rabbit IgG HRP conjugate
Predicted band size: 23 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
Datasheets and documents
Protocols
References
ab26935 has not yet been referenced specifically in any publications.
