Anti-White spot syndrome virus VP28 antibody (ab26935)
Key features and details
- Rabbit polyclonal to White spot syndrome virus VP28
- Suitable for: ELISA, WB
- Isotype: IgG
Overview
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Product name
Anti-White spot syndrome virus VP28 antibody -
Description
Rabbit polyclonal to White spot syndrome virus VP28 -
Host species
Rabbit -
Tested applications
Suitable for: ELISA, WBmore details -
Species reactivity
Reacts with: Other species -
Immunogen
Synthetic peptide corresponding to White spot syndrome virus VP28 aa 69-149. Synthetic peptide - corresponding to the VP 28 central domain (amino acids 69-148) of shrimp White spot syndrome virus.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: Whole serum -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab26935 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA |
1/5000.
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| WB |
1/400 - 1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 23 kDa).
Use at 1/400 for for tissue extracts/cell lysates, and at 1/1000 for recombinant protein. |
| Notes |
|---|
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ELISA
1/5000. |
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WB
1/400 - 1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 23 kDa). Use at 1/400 for for tissue extracts/cell lysates, and at 1/1000 for recombinant protein. |
Target
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Relevance
White spot syndrome virus (WSSV) occurs worldwide and causes high mortality and considerable economic damage to the shrimp farming industry. The VP28 was one of the envelope proteins of WSSV. Almost all shrimps infected with WSSV in ponds die within 3 to 4 days after the first dead shrimp was observed with gross lesions ranging from abnormal red body discoloration to white spots in the cuticle. -
Database links
- Entrez Gene: 927064 Other species
- SwissProt: Q4U3X7 Other species
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Alternative names
- Shrimp white spot syndrome virus VP 28 antibody
- Shrimp white spot syndrome virus VP28 antibody
- VP 28 antibody
see all
Images
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Western blot - Anti-White spot syndrome virus VP28 antibody (ab26935)All lanes : Anti-White spot syndrome virus VP28 antibody (ab26935) at 1/1000 dilution
Lane 1 : Lane (A M1) Control: E.Coli total cell lysate before IPTG induction, Pre bleed
Lane 2 : Lane (A M2) Recombinant VP28 protein expression after IPTG induction, Pre Bleed
Lane 3 : Lane (B M1) Control: E.Coli total cell lysate before IPTG induction, Post immunization.
Lane 4 : Lane (B M2) Recombinant VP28 protein expression after IPTG induction, Post immunization.
Secondary
All lanes : anti rabbit IgG HRP conjugate
Predicted band size: 23 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
Datasheets and documents
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Datasheet download
References (3)
ab26935 has been referenced in 3 publications.
- Ganganboina AB et al. Cargo encapsulated hepatitis E virus-like particles for anti-HEV antibody detection. Biosens Bioelectron 185:113261 (2021). PubMed: 33962156
- Nasrin F et al. Fluorometric virus detection platform using quantum dots-gold nanocomposites optimizing the linker length variation. Anal Chim Acta 1109:148-157 (2020). PubMed: 32252898
- Takemura K et al. Electrochemical detection of white spot syndrome virus with a silicone rubber disposable electrode composed of graphene quantum dots and gold nanoparticle-embedded polyaniline nanowires. J Nanobiotechnology 18:152 (2020). PubMed: 33109213
