Overview

  • Product name

    Anti-WHSC1/NSD2 antibody [29D1] - BSA and Azide free
    See all WHSC1/NSD2 primary antibodies
  • Description

    Mouse monoclonal [29D1] to WHSC1/NSD2 - BSA and Azide free
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Fusion protein corresponding to Human WHSC1/NSD2 aa 1-650.

  • Positive control

    • IHC-P: Human normal testis tissue. WB: HeLa and SHSY5Y whole cell lysates. IP: HepG2 cell lysate.
  • General notes

    ab235726 is a PBS only version of ab75359.

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Applications

Our Abpromise guarantee covers the use of ab235726 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 80 kDa (predicted molecular weight: 152 kDa).
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Probable histone methyltransferase (By similarity). May act as a transcription regulator that binds DNA and suppresses IL5 transcription.
  • Tissue specificity

    Widely expressed.
  • Involvement in disease

    Note=A chromosomal aberration involving WHSC1 is a cause of multiple myeloma tumors. Translocation t(4;14)(p16.3;q32.3) with IgH.
    Note=WHSC1 is located in the Wolf-Hirschhorn syndrome (WHS) critical region. WHS results from by sub-telomeric deletions in the short arm of chromosome 4. WHSC1 is deleted in every case, however deletion of linked genes contributes to both the severity of the core characteristics and the presence of the additional syndromic problems.
  • Sequence similarities

    Belongs to the histone-lysine methyltransferase family. SET2 subfamily.
    Contains 1 AWS domain.
    Contains 1 HMG box DNA-binding domain.
    Contains 4 PHD-type zinc fingers.
    Contains 1 post-SET domain.
    Contains 2 PWWP domains.
    Contains 1 SET domain.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Cytoplasm and Nucleus. Chromosome.
  • Information by UniProt
  • Database links

  • Alternative names

    • FLJ23286 antibody
    • IL5 promoter REII region binding protein antibody
    • KIAA1090 antibody
    • MGC176638 antibody
    • MMSET antibody
    • MMSET type II antibody
    • Multiple myeloma SET domain containing protein type III antibody
    • Multiple myeloma SET domain protein antibody
    • Multiple myeloma SET domain-containing protein antibody
    • NSD 2 antibody
    • NSD2 antibody
    • NSD2_HUMAN antibody
    • Nuclear receptor binding SET domain protein 2 antibody
    • Nuclear SET domain-containing protein 2 antibody
    • Probable histone-lysine N-methyltransferase NSD2 antibody
    • Protein trithorax-5 antibody
    • REIIBP antibody
    • Trithorax/ash1 related protein 5 antibody
    • TRX5 antibody
    • TRX5 protein antibody
    • WHS antibody
    • Whsc1 antibody
    • Wolf Hirschhorn syndrome candidate 1 antibody
    • Wolf Hirschhorn syndrome candidate 1 protein antibody
    • Wolf-Hirschhorn syndrome candidate 1 protein antibody
    see all

Images

  • ab75359 (1µg/ml) staining WHSC in human testis using an automated system (DAKO Autostainer Plus). Using this protocol there is strong nuclear staining.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH6.1/ in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, Azide and Arginine (ab75359).

  • WHSC1/NSD2 was immunoprecipitated using 0.5mg HepG2 whole cell extract, 10µg of Mouse monoclonal to WHSC1/NSD2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). 
    The antibody was incubated under agitation with Protein G beads for 10min, HepG2 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab75359.
    Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/5000 dilution.
    Band: 71kDa: WHSC1/NSD2.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, Azide and Arginine (ab75359).

References

ab235726 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab235726.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up