Product nameAnti-WHSC1/NSD2 antibody
See all WHSC1/NSD2 primary antibodies
DescriptionRabbit polyclonal to WHSC1/NSD2
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Macaque monkey, Gorilla
Synthetic peptide conjugated to KLH derived from within residues 500 - 600 of Human WHSC1/NSD2.
- This antibody gave a positive signal in the following human whole cell lysates: HeLa; Jurkat; HEK293; HepG2.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab106180 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 170 kDa (predicted molecular weight: 152 kDa).|
|IHC-P||Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionProbable histone methyltransferase (By similarity). May act as a transcription regulator that binds DNA and suppresses IL5 transcription.
Tissue specificityWidely expressed.
Involvement in diseaseNote=A chromosomal aberration involving WHSC1 is a cause of multiple myeloma tumors. Translocation t(4;14)(p16.3;q32.3) with IgH.
Note=WHSC1 is located in the Wolf-Hirschhorn syndrome (WHS) critical region. WHS results from by sub-telomeric deletions in the short arm of chromosome 4. WHSC1 is deleted in every case, however deletion of linked genes contributes to both the severity of the core characteristics and the presence of the additional syndromic problems.
Sequence similaritiesBelongs to the histone-lysine methyltransferase family. SET2 subfamily.
Contains 1 AWS domain.
Contains 1 HMG box DNA-binding domain.
Contains 4 PHD-type zinc fingers.
Contains 1 post-SET domain.
Contains 2 PWWP domains.
Contains 1 SET domain.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationCytoplasm and Nucleus. Chromosome.
- Information by UniProt
- FLJ23286 antibody
- IL5 promoter REII region binding protein antibody
- KIAA1090 antibody
All lanes : Anti-WHSC1/NSD2 antibody (ab106180) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 152 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted?
Additional bands at: 32 kDa, 55 kDa, 70 kDa (possible isoform). We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes
This antibody was raised against an immunogen that is predicted to recognize isoforms 1 and 3 of WHSC1/NSD2. The predicted molecular weights of isoforms 1 and 3 are 152 kDa and 71 kDa respectively.
IHC image of WHSC1/NSD2 staining in human testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab106180, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ab106180 has not yet been referenced specifically in any publications.