Product nameAnti-WHSC1/NSD2 antibody
See all WHSC1/NSD2 primary antibodies
DescriptionRabbit polyclonal to WHSC1/NSD2
Tested applicationsSuitable for: WBmore details
Unsuitable for: IP
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Gorilla
Synthetic peptide within Human WHSC1/NSD2 aa 525-575. The exact sequence is proprietary. (NP_579877.1).
Database link: O96028
- WB: HeLa whole cell lysate (ab150035).
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab225625 was affinity purified using an epitope specific to WHSC1/NSD2 immobilized on solid support.
Our Abpromise guarantee covers the use of ab225625 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 152 kDa.|
FunctionProbable histone methyltransferase (By similarity). May act as a transcription regulator that binds DNA and suppresses IL5 transcription.
Tissue specificityWidely expressed.
Involvement in diseaseNote=A chromosomal aberration involving WHSC1 is a cause of multiple myeloma tumors. Translocation t(4;14)(p16.3;q32.3) with IgH.
Note=WHSC1 is located in the Wolf-Hirschhorn syndrome (WHS) critical region. WHS results from by sub-telomeric deletions in the short arm of chromosome 4. WHSC1 is deleted in every case, however deletion of linked genes contributes to both the severity of the core characteristics and the presence of the additional syndromic problems.
Sequence similaritiesBelongs to the histone-lysine methyltransferase family. SET2 subfamily.
Contains 1 AWS domain.
Contains 1 HMG box DNA-binding domain.
Contains 4 PHD-type zinc fingers.
Contains 1 post-SET domain.
Contains 2 PWWP domains.
Contains 1 SET domain.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationCytoplasm and Nucleus. Chromosome.
- Information by UniProt
- FLJ23286 antibody
- IL5 promoter REII region binding protein antibody
- KIAA1090 antibody
All lanes : Anti-WHSC1/NSD2 antibody (ab225625) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 152 kDa
Exposure time: 30 seconds
ab225625 has not yet been referenced specifically in any publications.