Key features and details
- Rabbit polyclonal to WHSC2/NELF-A
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-WHSC2/NELF-A antibody
See all WHSC2/NELF-A primary antibodies
DescriptionRabbit polyclonal to WHSC2/NELF-A
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide within Human WHSC2/NELF-A aa 499-549. The exact sequence is proprietary.
Database link: Q9H3P2
- WB: HeLa whole cell lysate. IP: HeLa whole cell lysate. IHC-P: Human Placenta tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab85852 was affinity purified using an epitope specific to WHSC2/NELF-A immobilized on solid support.
Our Abpromise guarantee covers the use of ab85852 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 60 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionEssential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. Probably required to interact with the RNA polymerase II complex.
Tissue specificityUbiquitous. Expressed in heart, brain, placenta, liver, skeletal muscle, kidney and pancreas. Expressed at lower level in adult lung. Expressed in fetal brain, lung, liver and kidney.
Sequence similaritiesBelongs to the NELF-A family.
Contains 1 HDAg-like domain.
DomainThe HDAg-like domain is essential for transcriptional repression, and mediates the interaction with the RNA polymerase II complex.
- Information by UniProt
- FLJ10442 antibody
- FLJ25112 antibody
- Negative elongation factor A antibody
All lanes : Anti-WHSC2/NELF-A antibody (ab85852) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 60 kDa
Observed band size: 60 kDa
Exposure time: 3 minutes
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placenta tissue labelling WHSC2 with ab85852 at 1/1000 (1µg/ml). Detection: DAB.
WHSC2/NELF-A was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg/IP; 20% of IP loaded/lane) using ab85852 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab85852, at 1 µg/ml.
Lane 1: ab85852 IP in HeLa whole cell lysate.
Lane 2: Control IgG.
Detection: Chemiluminescence with exposure times of 3 minutes
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab85852 has been referenced in 1 publication.
- Awwad SW et al. NELF-E is recruited to DNA double-strand break sites to promote transcriptional repression and repair. EMBO Rep 18:745-764 (2017). PubMed: 28336775