Product nameWI-38 nuclear extract lysate (forskolin stimulated)
See all WI 38 lysates
The WI-38 nuclear extracts have been collected after a 30-minute incubation with or without forskolin (20 µM). The protein content has been determined by a Bradford-based assay.
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Storage bufferpH: 7.50
Constituents: 0.0002% Sodium molybdate, 0.216% Beta glycerophosphate, 0.018% Sodium orthovanadate, 0.042% Sodium fluoride, 0.015% DTT, 0.48% HEPES, 0.003% EDTA, 20% Glycerol, 2.34% Sodium chloride
With Protease inhibitor
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Each lot has been tested for CREB activation by using TransAM™ pCREB Kits. The signal intensity for CREB activation in each lot is compared to the signal intensity obtained with extracts from unstimulated WI-38 VA13 cells. After the signals are blanked, the ratio of the signals from stimulated cells over unstimulated cells must be above 3. This ratio may vary depending on the basal level of CREB activation in a given cell type.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab14912 has not yet been referenced specifically in any publications.