Product nameWI-38 nuclear extract lysate (TPA + CI stimulated)
See all WI 38 lysates
The protein content has been determined by a Bradford-based assay. The WI-38 nuclear extracts have been collected after a 2-hour incubation with or without TPA (100 ng/ml) and ionomycin (calcium ionophore) (2 µg/ml).
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Storage bufferpH: 7.50
Constituents: 0.0002% Sodium molybdate, 0.216% Beta glycerophosphate, 0.018% Sodium orthovanadate, 0.042% Sodium fluoride, 0.015% DTT, 0.48% HEPES, 0.003% EDTA, 20% Glycerol, 2.34% Sodium chloride
With Protease inhibitors
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To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab14913 has not yet been referenced specifically in any publications.