Anti-wide spectrum Cytokeratin antibody (ab9377)

Rabbit polyclonal wide spectrum Cytokeratin antibody. Validated in WB, IHC, ICC, Flow Cyt, ICC/IF and tested in Mouse, Rat, Cow, Dog, Human, Pig, Cynomolgus monkey, Common marmoset.

Overview

  • Product name

    Anti-wide spectrum Cytokeratin antibody
    See all wide spectrum Cytokeratin primary antibodies
  • Description

    Rabbit polyclonal to wide spectrum Cytokeratin
  • Host species

    Rabbit
  • Specificity

    This antibody reacts with cytokeratins of MW 58, 56, 52, 60, 51, 48 and 68 kDa.
  • Tested applications

    Suitable for: WB, IHC-P, IHC-Fr, ICC/IF, Flow Cyt, ICCmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Cow, Dog, Human, Pig, Cynomolgus monkey, Common marmoset
  • Immunogen

    Cytokeratin isolated from bovine muzzle epidermis.

Properties

Applications

Our Abpromise guarantee covers the use of ab9377 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Used at a dilution of 1/1000 overnight incubation (see Abreview for further information).
IHC-P Use at an assay dependent concentration. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. This antibody may be diluted to a titer of 1:75-1:200 in an ABC method.
IHC-Fr Use at an assay dependent concentration. This antibody may be diluted to a titer of 1/75 - 1/200 in an ABC method.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

ICC Use at an assay dependent concentration.

Target

  • Alternative names

    • pan cytokeratin antibody

Images

  • ab9377 staining wide spectrum Cytokeratin in formalin-fixed, paraffin-embedded Human skin tissue by Immunohistochemistry.
  • ab14404 staining wide spectrum Cytokeratin in the Human urether cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.2% Triton X-100 in PBS and blocked with 1% normal goat serum for 30 minutes at 20°C. Samples were incubated with primary antibody (1/200) for 16 hours. A Cy3®-conjugated Goat anti-rabbit polyclonal (1/800) was used as the secondary antibody.

    See Abreview

  • ab17976 staining wide spectrum Cytokeratin in Human skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with BlockAid for 1 hour at 20°C; antigen retrieval was by heat mediation in a citric buffer. Samples were incubated with primary antibody (1/500 in 1% BSA + PBS) for 16 hours at 4°C. An Alexa Fluor® 555-conjugated Goat anti-rabbit IgG (H+L) polyclonal (1/1000) was used as the secondary antibody.

    See Abreview

  • ab9377 staining wide spectrum Cytokeratin in rat liver tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with formaldehyde and blocked with 2% BSA for 10 minutes at 21°C and antigen retrieval was by heat mediation in citrate buffer. The sample was incubated with primary antibody (1/500 in TBS/BSA/azide) at 21°C for 2 hours. A biotin-conjugated goat anti-rabbit polyclonal (1/300) was used as the secondary antibody.

    See Abreview

  • Ab9377 positively staining paraformaldehyde fixed human retinal pigment epithelium derived from a fetal donor and established in primary culture(1/75). Ab9377 was used in conjunction with goat anti rabbit Alexa Ò488 (1/100).

    See Abreview

  • ab9377 staining wide spectrum Cytokeratin in common marmoset lung tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with formaldehyde and blocked with 2% BSA for 10 minutes at 21°C and antigen retrieval was by heat mediation in citrate buffer. The sample was incubated with primary antibody (1/500 in TBS/BSA/azide) at 21°C for 2 hours. A biotin-conjugated goat anti-rabbit polyclonal (1/300) was used as the secondary antibody.

    See Abreview

  • Ab9377 positively staining Cytokeratin (green) in paraffin fixed mouse oesophagus at 1/250.

     

    See Abreview

  • ab9377 staining mouse mesonephric epithelium cells by ICC/IF. Cells were methanol fixed and blocked in 1% BSA, 2% FBS in PBS for 20 minutes at 23°C prior to incubating with the primary antibody, diluted 1/75, for 18 hours at 4°C.  An Alexa Fluor® 488 conjugated donkey anti-rabbit antibody was used as the secondary.

    See Abreview

References

This product has been referenced in:

  • Venhuizen JH  et al. Differential expression of p120-catenin 1 and 3 isoforms in epithelial tissues. Sci Rep 9:90 (2019). Read more (PubMed: 30643202) »
  • Zhang S  et al. Implantation initiation of self-assembled embryo-like structures generated using three types of mouse blastocyst-derived stem cells. Nat Commun 10:496 (2019). Read more (PubMed: 30700702) »
See all 71 Publications for this product

Customer reviews and Q&As

1-10 of 54 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Uterus)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium citrate
Permeabilization
Yes - Tween-20
Specification
Uterus
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Fixative
Paraformaldehyde

Reshef Tal

Verified customer

Submitted Aug 15 2019

Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Tongue)
Permeabilization
No
Specification
Tongue
Blocking step
DAKO blocking solution as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.25% · Temperature: 24°C
Fixative
Acetone

Han-Byul Lee

Verified customer

Submitted May 27 2019

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Descending Colon)
Antigen retrieval step
Heat mediated
Permeabilization
No
Specification
Descending Colon
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Mar 25 2019

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Bos taurus Tissue sections (uterus)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA (pH 9.0)
Permeabilization
No
Specification
uterus
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Mar 01 2019

Application
Immunocytochemistry
Sample
Mouse Cell (Cancer cells were injected in the mammary epitheli)
Permeabilization
No
Specification
Cancer cells were injected in the mammary epitheli
Blocking step
10% Donkey Serum in 3% BSA/PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C
Fixative
Formaldehyde

Dr. Francesco Elia Marino

Verified customer

Submitted Sep 21 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Cat Tissue sections (skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
Permeabilization
No
Specification
skin
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: RT°C
Fixative
10% NBF

Abcam user community

Verified customer

Submitted Jul 06 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
Permeabilization
No
Specification
skin
Blocking step
Serum as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: RT°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Aug 29 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
Permeabilization
No
Specification
skin
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: RT°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Aug 29 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
Permeabilization
No
Specification
skin
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: RT°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Aug 29 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Marmoset (common) Tissue sections (Lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Lung
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde

Mr. Carl Hobbs

Verified customer

Submitted Aug 24 2016

1-10 of 54 Abreviews or Q&A

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