Anti-wide spectrum Cytokeratin antibody (ab9377)
Key features and details
- Rabbit polyclonal to wide spectrum Cytokeratin
- Suitable for: IHC-P, ICC, ICC/IF, Flow Cyt, WB, IHC-Fr
- Reacts with: Mouse, Rat, Cow, Dog, Human, Pig, Cynomolgus monkey, Common marmoset
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-wide spectrum Cytokeratin antibody -
Description
Rabbit polyclonal to wide spectrum Cytokeratin -
Host species
Rabbit -
Specificity
This antibody reacts with cytokeratins of MW 58, 56, 52, 60, 51, 48 and 68 kDa. -
Tested applications
Suitable for: IHC-P, ICC, ICC/IF, Flow Cyt, WB, IHC-Frmore details -
Species reactivity
Reacts with: Mouse, Rat, Cow, Dog, Human, Pig, Cynomolgus monkey, Common marmoset -
Immunogen
Full length native protein (purified) corresponding to Cow wide spectrum Cytokeratin. Cytokeratin isolated from bovine muzzle epidermis.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.3
Preservative: 0.1% Sodium azide
Constituents: 10% PBS, 1% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Light chain type
unknown -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab9377 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P | (28) |
Use at an assay dependent concentration. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. This antibody may be diluted to a titer of 1:75-1:200 in an ABC method.
|
ICC | (5) |
Use at an assay dependent concentration.
|
ICC/IF | (4) |
Use at an assay dependent concentration.
|
Flow Cyt |
Use at an assay dependent concentration.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB | (4) |
Use at an assay dependent concentration.
Used at a dilution of 1/1000 overnight incubation (see Abreview for further information). |
IHC-Fr | (2) |
Use at an assay dependent concentration.
This antibody may be diluted to a titer of 1/75 - 1/200 in an ABC method. |
Notes |
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IHC-P
Use at an assay dependent concentration. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. This antibody may be diluted to a titer of 1:75-1:200 in an ABC method. |
ICC
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
Use at an assay dependent concentration. Used at a dilution of 1/1000 overnight incubation (see Abreview for further information). |
IHC-Fr
Use at an assay dependent concentration. This antibody may be diluted to a titer of 1/75 - 1/200 in an ABC method. |
Target
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Alternative names
- pan cytokeratin antibody
Images
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ab9377 staining wide spectrum Cytokeratin in formalin-fixed, paraffin-embedded Human skin tissue by Immunohistochemistry.
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ab17976 staining wide spectrum Cytokeratin in Human skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with BlockAid for 1 hour at 20°C; antigen retrieval was by heat mediation in a citric buffer. Samples were incubated with primary antibody (1/500 in 1% BSA + PBS) for 16 hours at 4°C. An Alexa Fluor® 555-conjugated Goat anti-rabbit IgG (H+L) polyclonal (1/1000) was used as the secondary antibody.
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ab9377 staining wide spectrum Cytokeratin in rat liver tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with formaldehyde and blocked with 2% BSA for 10 minutes at 21°C and antigen retrieval was by heat mediation in citrate buffer. The sample was incubated with primary antibody (1/500 in TBS/BSA/azide) at 21°C for 2 hours. A biotin-conjugated goat anti-rabbit polyclonal (1/300) was used as the secondary antibody.
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ab9377 staining wide spectrum Cytokeratin in common marmoset lung tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with formaldehyde and blocked with 2% BSA for 10 minutes at 21°C and antigen retrieval was by heat mediation in citrate buffer. The sample was incubated with primary antibody (1/500 in TBS/BSA/azide) at 21°C for 2 hours. A biotin-conjugated goat anti-rabbit polyclonal (1/300) was used as the secondary antibody.
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Ab9377 positively staining Cytokeratin (green) in paraffin fixed mouse oesophagus at 1/250.
Datasheets and documents
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SDS download
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Datasheet download
References (161)
ab9377 has been referenced in 161 publications.
- Chen YH et al. Loss of cell-cell adhesion triggers cell migration through Rac1-dependent ROS generation. Life Sci Alliance 6:N/A (2023). PubMed: 36446524
- Moore J et al. Mammary Tumor-Derived Transplants as Breast Cancer Models to Evaluate Tumor-Immune Interactions and Therapeutic Responses. Cancer Res 82:365-376 (2022). PubMed: 34903599
- Below CR et al. A microenvironment-inspired synthetic three-dimensional model for pancreatic ductal adenocarcinoma organoids. Nat Mater 21:110-119 (2022). PubMed: 34518665
- Wang C et al. Low-cost polymer-film spiral inertial microfluidic device for label-free separation of malignant tumor cells. Electrophoresis 43:464-471 (2022). PubMed: 34611912
- Kanayama M et al. Prognostic impact of circulating tumor cells detected with the microfluidic "universal CTC-chip" for primary lung cancer. Cancer Sci 113:1028-1037 (2022). PubMed: 34964211