Overview

  • Product name

  • Description

    Rabbit polyclonal to WIF1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human WIF1. The exact sequence is proprietary.
    Database link: Q9Y5W5

  • Positive control

    • Extracts of mouse trachea, heart and skeletal muscle cell lines.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.3
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol, 49% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab186845 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB 1/500 - 1/2000. Predicted molecular weight: 41 kDa.
IHC-P 1/50 - 1/200.

Target

Images

  • Immunocytochemistry/Immunofluorescence analysis of U2OS cells using ab186845. Blue DAPI for nuclear staining.
  • All lanes : Anti-WIF1 antibody (ab186845) at 1/500 dilution

    Lane 1 : Extract of mouse trachea cell line
    Lane 2 : Extract of mouse heart cell line
    Lane 3 : Extract of mouse skeletal muscle cell line

    Predicted band size: 41 kDa

References

This product has been referenced in:

  • Farbehi N  et al. Single-cell expression profiling reveals dynamic flux of cardiac stromal, vascular and immune cells in health and injury. Elife 8:N/A (2019). Read more (PubMed: 30912746) »
  • Tang XB  et al. Expression pattern of Wif1 andß-catenin during development of anorectum in fetal rats with anorectal malformations. PeerJ 6:e4445 (2018). Read more (PubMed: 29507836) »
See all 5 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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