Overview

  • Product name

    Anti-WIF1 antibody [EPR9385]
    See all WIF1 primary antibodies
  • Description

    Rabbit monoclonal [EPR9385] to WIF1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: ICC/IF
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human WIF1. The exact sequence is proprietary.
    (Peptide available as ab171766)

  • Positive control

    • WB: HT29 and HeLa whole cell lysate (ab150035), human fetal heart and fetal lung tissue lysates, mouse and rat heart tissue lysates. IP: Human fetal lung tissue lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR9385
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab155101 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 41 kDa.Can be blocked with WIF1 peptide (ab171766).

For unpurified use at 1/1000 - 1/10000.

IP 1/60.

For unpurified use at 1/10 - 1/100.

  • Application notes
    Is unsuitable for ICC/IF.
  • Target

    Images

    • Anti-WIF1 antibody [EPR9385] (ab155101) at 1/2000 dilution (purified) + Human fetal heart tissue lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 41 kDa
      Observed band size: 41 kDa



      Blocking and diluting buffer: 5% NFDM/TBST.

    • Anti-WIF1 antibody [EPR9385] (ab155101) at 1/5000 dilution (purified) + Human fetal lung tissue lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 41 kDa
      Observed band size: 41 kDa



      Blocking and diluting buffer: 5% NFDM/TBST.

    • All lanes : Anti-WIF1 antibody [EPR9385] (ab155101) at 1/1000 dilution (purified)

      Lane 1 : Mouse heart tissue lysate
      Lane 2 : Rat heart tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 41 kDa
      Observed band size: 41 kDa



      Blocking and diluting buffer: 5% NFDM/TBST.

    • All lanes : Anti-WIF1 antibody [EPR9385] (ab155101) at 1/1000 dilution (unpurified)

      Lane 1 : HT29 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : Human fetal lung tissue lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 41 kDa

    • All lanes : Anti-WIF1 antibody [EPR9385] (ab155101) at 1/1000 dilution (unpurified)

      Lanes 1-2 : pmHep3B whole cell lysate
      Lanes 3-4 : Hep3B whole cell lysate

      Lysates/proteins at 30 µg per lane.

      Secondary
      All lanes : Alkaline Phosphatase conjugated goat anti-rabbit IgG polyclonal at 1/15000 dilution

      Developed using the ECL technique.

      Performed under non-reducing conditions.

      Predicted band size: 41 kDa
      Observed band size: 41 kDa


      Exposure time: 1 minute

      See Abreview

    • Purified ab155101 (1/60) immunoprecipitating WIF1 in human fetal lung whole cell lysate (Lane +). For western blotting an Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG (1/500) was used as the secondary antibody.

      Lane (-): Rabbit monoclonal IgG (ab172730) instead of ab155101 in Human fetal lung whole cell lysate.

      Blocking and diluting buffer: 5% NFDM/TBST.

    References

    This product has been referenced in:

    • Zhou M  et al. Polygonum multiflorm alleviates glucocorticoid-induced osteoporosis and Wnt signaling pathway. Mol Med Rep 17:970-978 (2018). Read more (PubMed: 29115514) »
    • Li C  et al. MicroRNA-552 promotes hepatocellular carcinoma progression by downregulating WIF1. Int J Mol Med 42:3309-3317 (2018). Read more (PubMed: 30221686) »
    See all 7 Publications for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    This product is known to not work in this application or species.
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Sample
    Mouse Cell (Hepatocyte)
    Specification
    Hepatocyte
    Permeabilization
    No
    Fixative
    Paraformaldehyde

    Abcam user community

    Verified customer

    Submitted Feb 20 2014

    Application
    Western blot
    Loading amount
    30 µg
    Gel Running Conditions
    Non-reduced Denaturing
    Sample
    Human Cell lysate - whole cell (Hep3B)
    Specification
    Hep3B
    Blocking step
    I-Block(Applied biosystems) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2µg/mL · Temperature: 22°C

    Abcam user community

    Verified customer

    Submitted Jan 13 2014

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up