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RecombinantRabMAb

Recombinant Anti-WIF1 antibody [EPR9385] (ab155101)

Reviews (2) Submit a question References (14)
Western blot - Anti-WIF1 antibody [EPR9385] (ab155101)
  • Western blot - Anti-WIF1 antibody [EPR9385] (ab155101)
  • Western blot - Anti-WIF1 antibody [EPR9385] (ab155101)
  • Western blot - Anti-WIF1 antibody [EPR9385] (ab155101)
  • Western blot - Anti-WIF1 antibody [EPR9385] (ab155101)
  • Immunoprecipitation - Anti-WIF1 antibody [EPR9385] (ab155101)
  • Anti-WIF1 antibody [EPR9385] (ab155101)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9385] to WIF1
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-WIF1 antibody [EPR9385]
    See all WIF1 primary antibodies

  • Description

    Rabbit monoclonal [EPR9385] to WIF1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: ICC/IF

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human WIF1. The exact sequence is proprietary.
    (Peptide available as ab171766)

  • Positive control

    • WB: HT29 and HeLa whole cell lysate (ab150035), human fetal heart and fetal lung tissue lysates, mouse and rat heart tissue lysates. IP: Human fetal lung tissue lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR9385

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab155101 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
1/2000. Predicted molecular weight: 41 kDa.Can be blocked with Human WIF1 peptide (ab171766).

For unpurified use at 1/1000 - 1/10000.
IP
1/60.

For unpurified use at 1/10 - 1/100.
Notes
WB
1/2000. Predicted molecular weight: 41 kDa.Can be blocked with Human WIF1 peptide (ab171766).

For unpurified use at 1/1000 - 1/10000.
IP
1/60.

For unpurified use at 1/10 - 1/100.
Application notes
Is unsuitable for ICC/IF.

Target

Images

  • Western blot - Anti-WIF1 antibody [EPR9385] (ab155101)
    Western blot - Anti-WIF1 antibody [EPR9385] (ab155101)
    Anti-WIF1 antibody [EPR9385] (ab155101) at 1/2000 dilution (purified) + Human fetal heart tissue lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 41 kDa
    Observed band size: 41 kDa



    Blocking and diluting buffer: 5% NFDM/TBST.

  • Western blot - Anti-WIF1 antibody [EPR9385] (ab155101)
    Western blot - Anti-WIF1 antibody [EPR9385] (ab155101)
    Anti-WIF1 antibody [EPR9385] (ab155101) at 1/5000 dilution (purified) + Human fetal lung tissue lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 41 kDa
    Observed band size: 41 kDa



    Blocking and diluting buffer: 5% NFDM/TBST.

  • Western blot - Anti-WIF1 antibody [EPR9385] (ab155101)
    Western blot - Anti-WIF1 antibody [EPR9385] (ab155101)
    All lanes : Anti-WIF1 antibody [EPR9385] (ab155101) at 1/1000 dilution (purified)

    Lane 1 : Mouse heart tissue lysate
    Lane 2 : Rat heart tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 41 kDa
    Observed band size: 41 kDa



    Blocking and diluting buffer: 5% NFDM/TBST.

  • Western blot - Anti-WIF1 antibody [EPR9385] (ab155101)
    Western blot - Anti-WIF1 antibody [EPR9385] (ab155101)
    All lanes : Anti-WIF1 antibody [EPR9385] (ab155101) at 1/1000 dilution (unpurified)

    Lane 1 : HT29 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : Human fetal lung tissue lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 41 kDa

  • Western blot - Anti-WIF1 antibody [EPR9385] (ab155101)
    Western blot - Anti-WIF1 antibody [EPR9385] (ab155101)This image is courtesy of an anonymous Abreview
    All lanes : Anti-WIF1 antibody [EPR9385] (ab155101) at 1/1000 dilution (unpurified)

    Lanes 1-2 : pmHep3B whole cell lysate
    Lanes 3-4 : Hep3B whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : Alkaline Phosphatase conjugated goat anti-rabbit IgG polyclonal at 1/15000 dilution

    Developed using the ECL technique.

    Performed under non-reducing conditions.

    Predicted band size: 41 kDa
    Observed band size: 41 kDa


    Exposure time: 1 minute

    See Abreview

  • Immunoprecipitation - Anti-WIF1 antibody [EPR9385] (ab155101)
    Immunoprecipitation - Anti-WIF1 antibody [EPR9385] (ab155101)

    Purified ab155101 (1/60) immunoprecipitating WIF1 in human fetal lung whole cell lysate (Lane +). For western blotting an Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG (1/500) was used as the secondary antibody.

    Lane (-): Rabbit monoclonal IgG (ab172730) instead of ab155101 in Human fetal lung whole cell lysate.

    Blocking and diluting buffer: 5% NFDM/TBST.

  • Anti-WIF1 antibody [EPR9385] (ab155101)
    Anti-WIF1 antibody [EPR9385] (ab155101)

References (14)

Publishing research using ab155101? Please let us know so that we can cite the reference in this datasheet.

ab155101 has been referenced in 14 publications.

  • Li K  et al. miR-340 Promotes Retinoblastoma Cell Proliferation, Migration and Invasion Through Targeting WIF1. Onco Targets Ther 14:3635-3648 (2021). PubMed: 34113129
  • Becker M  et al. WIF1 Suppresses the Generation of Suprabasal Cells in Acanthotic Skin and Growth of Basal Cell Carcinomas upon Forced Overexpression. J Invest Dermatol 140:1556-1565.e11 (2020). PubMed: 31987884
  • Niu J  et al. Aberrant oligodendroglial-vascular interactions disrupt the blood-brain barrier, triggering CNS inflammation. Nat Neurosci 22:709-718 (2019). PubMed: 30988524
  • Li J  et al. Impact of SLC20A1 on the Wnt/ß-catenin signaling pathway in somatotroph adenomas. Mol Med Rep 20:3276-3284 (2019). PubMed: 31432167
  • Xia P  et al. MicroRNA-377 exerts a potent suppressive role in osteosarcoma through the involvement of the histone acetyltransferase 1-mediated Wnt axis. J Cell Physiol 234:22787-22798 (2019). PubMed: 31152456
View all Publications for this product

Customer reviews and Q&As

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1-2 of 2 Abreviews

Immunocytochemistry/ Immunofluorescence abreview for Anti-WIF1 antibody [EPR9385]

 Good
Abreviews
This product is known to not work in this application or species.
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Sample
Mouse Cell (Hepatocyte)
Specification
Hepatocyte
Permeabilization
No
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 20 2014

Western blot abreview for Anti-WIF1 antibody [EPR9385]

 Excellent
Abreviews
abreview image
Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Non-reduced Denaturing
Sample
Human Cell lysate - whole cell (Hep3B)
Specification
Hep3B
Blocking step
I-Block(Applied biosystems) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2µg/mL · Temperature: 22°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jan 13 2014

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