Recombinant Anti-WIF1 antibody [EPR9385] - BSA and Azide free (ab249191)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9385] to WIF1 - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-WIF1 antibody [EPR9385] - BSA and Azide free
See all WIF1 primary antibodies -
Description
Rabbit monoclonal [EPR9385] to WIF1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: ICC/IF -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
(Peptide available asab171766) -
General notes
ab249191 is the carrier-free version of ab155101.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9385 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Immunizing Peptide (Blocking)
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab249191 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use at an assay dependent concentration. Predicted molecular weight: 41 kDa.Can be blocked with Human WIF1 peptide (ab171766).
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IP |
Use at an assay dependent concentration.
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Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 41 kDa.Can be blocked with Human WIF1 peptide (ab171766). |
IP
Use at an assay dependent concentration. |
Target
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Function
Binds to WNT proteins and inhibits their activities. May be involved in mesoderm segmentation. -
Sequence similarities
Contains 5 EGF-like domains.
Contains 1 WIF domain. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 11197 Human
- Entrez Gene: 24117 Mouse
- Entrez Gene: 114557 Rat
- Omim: 605186 Human
- SwissProt: Q9Y5W5 Human
- SwissProt: Q9WUA1 Mouse
- SwissProt: Q6IN38 Rat
- Unigene: 284122 Human
see all -
Alternative names
- WIF-1 antibody
- wif1 antibody
- WIF1_HUMAN antibody
- Wnt inhibitory factor 1 antibody
Images
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Anti-WIF1 antibody [EPR9385] (ab155101) at 1/2000 dilution (purified) + Human fetal heart tissue lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 41 kDa
Observed band size: 41 kDaThis data was developed using ab155101, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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Anti-WIF1 antibody [EPR9385] (ab155101) at 1/5000 dilution (purified) + Human fetal lung tissue lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 41 kDa
Observed band size: 41 kDaThis data was developed using ab155101, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-WIF1 antibody [EPR9385] (ab155101) at 1/1000 dilution (purified)
Lane 1 : Mouse heart tissue lysate
Lane 2 : Rat heart tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 41 kDa
Observed band size: 41 kDaThis data was developed using ab155101, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-WIF1 antibody [EPR9385] (ab155101) at 1/1000 dilution (unpurified)
Lane 1 : HT29 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Human fetal lung tissue lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 41 kDaThis data was developed using ab155101, the same antibody clone in a different buffer formulation.
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This data was developed using ab155101, the same antibody clone in a different buffer formulation.
Purified ab155101 (1/60) immunoprecipitating WIF1 in human fetal lung whole cell lysate (Lane +). For western blotting an Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG (1/500) was used as the secondary antibody.
Lane (-): Rabbit monoclonal IgG (ab172730) instead of ab155101 in Human fetal lung whole cell lysate.
Blocking and dilution buffer: 5% NFDM/TBST.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab249191 has not yet been referenced specifically in any publications.