Overview

  • Product name

    Anti-Wilms Tumor Protein antibody [5G11A5]
    See all Wilms Tumor Protein primary antibodies
  • Description

    Mouse monoclonal [5G11A5] to Wilms Tumor Protein
  • Host species

    Mouse
  • Tested applications

    Suitable for: Flow Cyt, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human Wilms Tumor Protein aa 314-479.

  • Positive control

    • Human Wilms Tumor Protein (aa 314-479) recombinant protein; Wilms Tumor Protein (aa 314-479)-hIgGFc transfected HEK293 (2) cell lysate; HeLa cells.
  • General notes

    This product was changed from ascites to supernatant. Lot no’s high than GR278304-7 are from Tissue Culture Supernatant

Properties

Applications

Our Abpromise guarantee covers the use of ab201948 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/200 - 1/400.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

WB 1/500 - 1/2000. Predicted molecular weight: 49 kDa.

Target

  • Function

    Transcription factor that plays an important role in cellular development and cell survival. Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. Recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3'. It has a tumor suppressor as well as an oncogenic role in tumor formation. Function may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors. Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing. Isoform 1 has lower affinity for DNA, and can bind RNA.
  • Tissue specificity

    Expressed in the kidney and a subset of hematopoietic cells.
  • Involvement in disease

    Defects in WT1 are the cause of Frasier syndrome (FS) [MIM:136680]. FS is characterized by a slowly progressing nephropathy leading to renal failure in adolescence or early adulthood, male pseudohermaphroditism, and no Wilms tumor. As for histological findings of the kidneys, focal glomerular sclerosis is often observed. There is phenotypic overlap with Denys-Drash syndrome. Inheritance is autosomal dominant.
    Defects in WT1 are the cause of Wilms tumor 1 (WT1) [MIM:194070]. WT is an embryonal malignancy of the kidney that affects approximately 1 in 10'000 infants and young children. It occurs both in sporadic and hereditary forms.
    Defects in WT1 are the cause of Denys-Drash syndrome (DDS) [MIM:194080]. DDS is a typical nephropathy characterized by diffuse mesangial sclerosis, genital abnormalities, and/or Wilms tumor. There is phenotypic overlap with WAGR syndrome and Frasier syndrome. Inheritance is autosomal dominant, but most cases are sporadic.
    Defects in WT1 are the cause of nephrotic syndrome type 4 (NPHS4) [MIM:256370]. A renal disease characterized clinically by proteinuria, hypoalbuminemia, hyperlipidemia and edema. Kidney biopsies show non-specific histologic changes such as focal segmental glomerulosclerosis and diffuse mesangial proliferation. Some affected individuals have an inherited steroid-resistant form and progress to end-stage renal failure. Most patients with NPHS4 show diffuse mesangial sclerosis on renal biopsy, which is a pathologic entity characterized by mesangial matrix expansion with no mesangial hypercellularity, hypertrophy of the podocytes, vacuolized podocytes, thickened basement membranes, and diminished patency of the capillary lumen.
    Defects in WT1 are a cause of Meacham syndrome (MEACHS) [MIM:608978]. Meacham syndrome is a rare sporadically occurring multiple malformation syndrome characterized by male pseudohermaphroditism with abnormal internal female genitalia comprising a uterus and double or septate vagina, complex congenital heart defect and diaphragmatic abnormalities.
    Note=A chromosomal aberration involving WT1 may be a cause of desmoplastic small round cell tumor (DSRCT). Translocation t(11;22)(p13;q12) with EWSR1.
  • Sequence similarities

    Belongs to the EGR C2H2-type zinc-finger protein family.
    Contains 4 C2H2-type zinc fingers.
  • Cellular localization

    Nucleus. Cytoplasm. Shuttles between nucleus and cytoplasm; Nucleus > nucleoplasm and Nucleus speckle.
  • Information by UniProt
  • Database links

  • Alternative names

    • WIT 2 antibody
    • WT 1 antibody
    • AWT1 antibody
    • FWT1 antibody
    • GUD antibody
    • NPHS4 antibody
    • WAGR antibody
    • Wilms tumor 1 antibody
    • Wilms Tumor antibody
    • Wilms tumor protein antibody
    • Wilms' tumor gene antibody
    • Wilms' tumor protein antibody
    • WIT2 antibody
    • WT antibody
    • WT1 antibody
    • WT1_HUMAN antibody
    • WT33 antibody
    see all

Images

  • Anti-Wilms Tumor Protein antibody [5G11A5] (ab201948) at 1/500 dilution + Human Wilms Tumor Protein (aa314-479) recombinant protein

    Predicted band size: 49 kDa



    Expected MWt is 47.6 kDa.

  • All lanes : Anti-Wilms Tumor Protein antibody [5G11A5] (ab201948) at 1/500 dilution

    Lane 1 : HEK293 cell lysate
    Lane 2 : Wilms Tumor Protein (aa314-479)-hIgGFc transfected HEK293 (2) cell lysate

    Predicted band size: 49 kDa

  • Flow cytometric analysis of HeLa cells labeling Wilms Tumor Protein with ab201948 at 1/200 dilution (green) compared to a negative control (red).

References

This product has been referenced in:

  • Yang HJ  et al. GP73 promotes invasion and metastasis of bladder cancer by regulating the epithelial-mesenchymal transition through the TGF-ß1/Smad2 signalling pathway. J Cell Mol Med 22:1650-1665 (2018). WB ; Human . Read more (PubMed: 29349903) »
  • Pons M  et al. Human platelet lysate as validated replacement for animal serum to assess chemosensitivity. ALTEX N/A:N/A (2018). Read more (PubMed: 30570667) »
See all 2 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Human pluripotent SC derived epicardial like cells)
Permeabilization
Yes - Saponin
Specification
Human pluripotent SC derived epicardial like cells
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 4% · Temperature: 4°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Nov 11 2016

Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Human foetal heart)
Permeabilization
Yes - 0.5% Triton-X
Specification
Human foetal heart
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jul 13 2016

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