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    wilms-tumor-protein-antibody-ab15249.pdf

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Tags & Cell Markers Cell Type Markers Tumor Associated
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Anti-Wilms Tumor Protein antibody (ab15249)

  • Datasheet
  • SDS
Reviews (2)Q&A (15)References (28)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wilms Tumor Protein antibody (ab15249)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wilms Tumor Protein antibody (ab15249)

Key features and details

  • Rabbit polyclonal to Wilms Tumor Protein
  • Suitable for: IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Wilms Tumor Protein antibody
    See all Wilms Tumor Protein primary antibodies
  • Description

    Rabbit polyclonal to Wilms Tumor Protein
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human Wilms Tumor Protein aa 400 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P19544

  • Positive control

    • IHC-P: FFPE Human Wilms Tumor Protein tissue sections, Human normal Testis tissue sections
  • General notes

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.60
    Preservative: 0.1% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Cell Type Markers
    • Tumor Associated
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Cancer
    • Tumor biomarkers
    • Other
    • Developmental Biology
    • Organogenesis
    • Excretory system development
    • Kidney development

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Wilms Tumor Protein (ab82233)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab15249 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
All applications
Mouse
Rat
Application Abreviews Notes
IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Transcription factor that plays an important role in cellular development and cell survival. Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. Recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3'. It has a tumor suppressor as well as an oncogenic role in tumor formation. Function may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors. Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing. Isoform 1 has lower affinity for DNA, and can bind RNA.
  • Tissue specificity

    Expressed in the kidney and a subset of hematopoietic cells.
  • Involvement in disease

    Defects in WT1 are the cause of Frasier syndrome (FS) [MIM:136680]. FS is characterized by a slowly progressing nephropathy leading to renal failure in adolescence or early adulthood, male pseudohermaphroditism, and no Wilms tumor. As for histological findings of the kidneys, focal glomerular sclerosis is often observed. There is phenotypic overlap with Denys-Drash syndrome. Inheritance is autosomal dominant.
    Defects in WT1 are the cause of Wilms tumor 1 (WT1) [MIM:194070]. WT is an embryonal malignancy of the kidney that affects approximately 1 in 10'000 infants and young children. It occurs both in sporadic and hereditary forms.
    Defects in WT1 are the cause of Denys-Drash syndrome (DDS) [MIM:194080]. DDS is a typical nephropathy characterized by diffuse mesangial sclerosis, genital abnormalities, and/or Wilms tumor. There is phenotypic overlap with WAGR syndrome and Frasier syndrome. Inheritance is autosomal dominant, but most cases are sporadic.
    Defects in WT1 are the cause of nephrotic syndrome type 4 (NPHS4) [MIM:256370]. A renal disease characterized clinically by proteinuria, hypoalbuminemia, hyperlipidemia and edema. Kidney biopsies show non-specific histologic changes such as focal segmental glomerulosclerosis and diffuse mesangial proliferation. Some affected individuals have an inherited steroid-resistant form and progress to end-stage renal failure. Most patients with NPHS4 show diffuse mesangial sclerosis on renal biopsy, which is a pathologic entity characterized by mesangial matrix expansion with no mesangial hypercellularity, hypertrophy of the podocytes, vacuolized podocytes, thickened basement membranes, and diminished patency of the capillary lumen.
    Defects in WT1 are a cause of Meacham syndrome (MEACHS) [MIM:608978]. Meacham syndrome is a rare sporadically occurring multiple malformation syndrome characterized by male pseudohermaphroditism with abnormal internal female genitalia comprising a uterus and double or septate vagina, complex congenital heart defect and diaphragmatic abnormalities.
    Note=A chromosomal aberration involving WT1 may be a cause of desmoplastic small round cell tumor (DSRCT). Translocation t(11;22)(p13;q12) with EWSR1.
  • Sequence similarities

    Belongs to the EGR C2H2-type zinc-finger protein family.
    Contains 4 C2H2-type zinc fingers.
  • Cellular localization

    Nucleus. Cytoplasm. Shuttles between nucleus and cytoplasm; Nucleus > nucleoplasm and Nucleus speckle.
  • Target information above from: UniProt accession P19544 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 7490 Human
    • Entrez Gene: 22431 Mouse
    • Entrez Gene: 24883 Rat
    • Omim: 607102 Human
    • SwissProt: P19544 Human
    • SwissProt: P22561 Mouse
    • SwissProt: P49952 Rat
    • Unigene: 591980 Human
    • Unigene: 389339 Mouse
    • Unigene: 92531 Rat
    see all
  • Alternative names

    • WIT 2 antibody
    • WT 1 antibody
    • AWT1 antibody
    • FWT1 antibody
    • GUD antibody
    • NPHS4 antibody
    • WAGR antibody
    • Wilms tumor 1 antibody
    • Wilms Tumor antibody
    • Wilms tumor protein antibody
    • Wilms' tumor gene antibody
    • Wilms' tumor protein antibody
    • WIT2 antibody
    • WT antibody
    • WT1 antibody
    • WT1_HUMAN antibody
    • WT33 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wilms Tumor Protein antibody (ab15249)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wilms Tumor Protein antibody (ab15249)

    IHC image of Wilms Tumor Protein staining in a section of formalin-fixed paraffin-embedded Human Wilms Tumor performed on a Ventana Discovery Ultra system using the standard protocol. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20mins. The section was then incubated with ab15249, 1/100, for 16 mins at 37°C and detected using an HRP conjugated anti-rabbit secondary antibody. DAB was used as the chromogen and the section was then counterstained with haematoxylin.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wilms Tumor Protein antibody (ab15249)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wilms Tumor Protein antibody (ab15249)

    IHC image of Wilms Tumor Protein staining in a section of formalin-fixed paraffin-embedded normal human kidney* performed on a Leica BONDTM system using the standard protocol. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab15249, 1/100, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

Protocols

  • Immunoprecipitation protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (28)

    Publishing research using ab15249? Please let us know so that we can cite the reference in this datasheet.

    ab15249 has been referenced in 28 publications.

    • Stock AT  et al. The Selective Expansion and Targeted Accumulation of Bone Marrow-Derived Macrophages Drive Cardiac Vasculitis. J Immunol 202:3282-3296 (2019). PubMed: 31004011
    • Petrosyan A  et al. A glomerulus-on-a-chip to recapitulate the human glomerular filtration barrier. Nat Commun 10:3656 (2019). PubMed: 31409793
    • Nilsson D  et al. Foxc2 is essential for podocyte function. Physiol Rep 7:e14083 (2019). PubMed: 31062503
    • Wanner N  et al. DNA Methyltransferase 1 Controls Nephron Progenitor Cell Renewal and Differentiation. J Am Soc Nephrol 30:63-78 (2019). PubMed: 30518531
    • Armelloni S  et al. NeuroD Expression in Podocytes and Interrelationships with Nephrin at Both Nuclear and Cytoplasmic Sites. Cell Physiol Biochem 46:873-889 (2018). PubMed: 29669318
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 17 Abreviews or Q&A

    IHC - Wholemount abreview for Anti-Wilms Tumor Protein antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    IHC - Wholemount
    Sample
    Mouse Embryo (lung, mesothelial cells)
    Specification
    lung, mesothelial cells
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 14 2016

    Immunohistochemistry (Frozen sections) abreview for Anti-Wilms Tumor Protein antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (lung, mesothelial cells)
    Permeabilization
    No
    Specification
    lung, mesothelial cells
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 14 2016

    Question

    This antibody did not work in Western blot as originally thought it would.

    Read More

    Abcam community

    Verified customer

    Asked on Apr 19 2012

    Answer

    Thank you for your phone call today, and I apologize for the troublewith this antibody.

    Your credit note ID is ***

    If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we may gather the correct information needed for the refund. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used.

    Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department.

    I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expertassistance or advice.

    Read More

    Abcam Scientific Support

    Answered on Apr 19 2012

    Question

    Thanks for your mail. I used two anti-WT-1 antibodies (ab15249 and ab64681) and both are giving multiple bands with my experiments. The ab64681 is giving one huge band at 100 KDa along with other additional bands that are alsotec observed with ab15249.I wrote my last mail as you asked forfeedback onyour antibodies.When I compared I find ab15249better than ab64681 with my experimental system, although it also produced multiple bands.I am still confused with the occurrence of these bands.

    Read More

    Abcam community

    Verified customer

    Asked on Feb 06 2012

    Answer

    Thank you for your reply. To ensure that your message gets directed to the person who has been handling your case, please do not alter the subject line when replying to our emails. This will allow your case to be tracked effectively in our system.
    After reviewing the details of your contacts with my colleagues, I have only located the image of ab73466 with ab64681. It appears that when running the peptide ab73466 in SDS-PAGE, you detected a band around 75 kDa. While ab73466 is suitable for blocking the specificity of ab64681 after preincubation, it is only 18 amino acids in length and is therefore too small to be detected in SDS-PAGE.
    This peptide is 70-90% pure and is supplied in a buffer that does not contain any additional proteins. Because this protein is synthetic, the 10-30% contamination will be amino acids, chemicals, and partial peptides, none of which would be expected to run at 75 kDa. The The presence of the 75 kDa band indicates that there may be some protein contamination in the loading or running buffer. This may also help to explain some of the additional bands in your western.
    According to the UniProt database, there are 8 isoforms of Wilms Tumor Protein with molecular weights ranging from 34 - 57 kDa. This protein is also known to interact with other proteins and to form homodimers (which may be the band observed at 100 kDa). Using a buffer with ionic and non-ionic detergents (such as RIPA buffer) and boiling the protein in the presence of BME or DTT will help to ensure that all multimers are broken down.
    I hope this helps, please let me know if you need any additional information or assistance.

    Read More

    Abcam Scientific Support

    Answered on Feb 06 2012

    Question

    I have asked for peptide sequence that was used to raise ab15249. In case it is not available, could you please provide me its sequence? I will be so thankful to you if you can provide that.

    Read More

    Abcam community

    Verified customer

    Asked on Feb 01 2012

    Answer

    Thank you for contacting us.

    The exact immunogen sequence is confidential, but it is contained within the range between aa 389-449 of P19544 (Uniprot), isoform 1.

    http://www.uniprot.org/uniprot/P19544

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Feb 01 2012

    Question

    I have received a product recall notification on ab15249.

    Read More

    Abcam community

    Verified customer

    Asked on Jan 27 2012

    Answer

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement.
    To check the status of the order please contact our Customer Service team and reference this number.
    Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.
    I wish you the best of luck with your research.

    Read More

    Abcam Scientific Support

    Answered on Jan 27 2012

    Question

    Dear Technical,
    Hello Sci Supp! The below customer called in response to an email from Abcam regarding (recalls I think). She wants to order replacements. I informed her that our technical team would contact her today to get more information.
    Customer Name:
    Customer Email:
    Abcam orders: #
    Products in question:
    ab15249 : Rabbit polyclonal to Wilms Tumor Protein
    ab65835 : Rabbit polyclonal to ANGPT1 + ANGPT2
    Thanks,

    Read More

    Abcam community

    Verified customer

    Asked on Jan 24 2012

    Answer

    Thank you for contacting Abcam.
    For ab15429 we have found that there are problems with this antibody in western blot and that we can no longer guarantee that it will work in this application. However it is still guaranteed to work in IP and IHC-P. If you had originally purchased this antibody to work in WB and you are having problems with it, then I will be happy to replace the antibody.
    As for ab65835 we have found that cross reacting with angiopoietin 1 and therefore we would be happy to either replace this antibody with another angiopoietin 1 antibody of your choice, possible ab8451 or ab94684.
    I do apologize for any inconvenience these issues may have caused you and I look forward to hearing from you so that I can get this sorted out.
    If there is anything I can help with, please let me know.

    Read More

    Abcam Scientific Support

    Answered on Jan 24 2012

    Question

    I checked the datasheets online, the ab96792 should work. Thank you for your help, I'll keep in mind to contact Prodotti Gianni for our next orders, Best regards,

    Read More

    Abcam community

    Verified customer

    Asked on Dec 16 2011

    Answer

    Thank you for your response. This is to let you know that I have confirmed and agreed on placing a new order for your customer - for one vial of ab96792 as a free of charge replacement. I hope the second vial will work as it is expected, and please do let me know how you are getting on with this product. Please could you contact Prodotti Gianni and discuss this case with them and also get some further information about the exact shipping time. Their contact details can be found at the website: www.prodottigianni.com Merry Xmas and Happy New Year!

    Read More

    Abcam Scientific Support

    Answered on Dec 16 2011

    Question

    Thank you for your prompt reply. I haven't contacted you before because, as I said, we purchased this antibody both for immunostaining and western blotting and I've been using it so far just for stainings. Today, by change, just 1day before the 6 months from the arrival date (16/6/2011) , I checked the Scientific Support session to find more information on how to use the antibody for WB. This is the technical support answer to question number 6: " ANSWER:   Thank you for contacting Abcam. I have talked to the people in the lab and also reviewed all the data we have on ab15249 and it seems as though this antibody does not work in western blot (it gives multiple bands, which is what you are seeing). The tested in western blot application was added to the datasheet by mistake. Please accept my apologies for this mistake and I hope it has not caused you too many problems. We do have several other Wilms Tumor Protein antibodies in our catalogue and I would be very happy to send you a replacement antibody. Several antibodies that you may be interested in are: - ab64681 - ab89901 - ab96792 Once again please accept my apologies and let me know which antibody you would like to receive as a replacement. Or if you would prefer I can process a refund. I look forward to your reply. which means that the western blot application has been added to the datasheet by mistake and even if I try for months I won't have a single band on the gel and most likely this won't be related to problems with the shipping or with the storage of the product. That's why I was hoping to have it replaced with another anybody which actually works for both the applications as specified from the datasheet. Regards,  

    Read More

    Abcam community

    Verified customer

    Asked on Dec 16 2011

    Answer

    Thank you for getting back to me and for clarifying the situation further. It is indeed the case that after consideration Western blot application has been removed from the datasheet. Although, you have purchased this antibody via an unauthorized reseller, I could certainly offer you an alternative antibody against Wilms Tumor Protein i.e. ab89901, ab118873. Please take a look at the datasheets and also our on-line catalogue and let me know which product you would prefer to get as a free of charge replacement. It is important to mention that our authorized distributor in Italy is Prodotti Gianni SpA (www.prodottigianni.com) so we can ship the product to them - even though originally you ordered this antibody via another company. I hope this would be a satisfactory outcome for you. I look forward to receiving confirmation that you would like to proceed with a replacement vial.

    Read More

    Abcam Scientific Support

    Answered on Dec 16 2011

    Question

    Thanks for your concern. You can send me one replaced vial of ab64681 along with the blocking peptide (ab73466) urgently to execute my experimets. It would have been much better if you could send me both replaced vials since the first vial was ordered long back and I am unable to find the order copy for that now. But since I have two vials of ab15249 and therefore it is obvious that I purchased two vials from you.    Thanks,  

    Read More

    Abcam community

    Verified customer

    Asked on Nov 08 2011

    Answer

    Thank you for your patience in this matter. I have processed the request for you to receive ab64681 as a replacement for ab15249 which did not work in western blot as stated on the datasheet. I have requested that the peptide ab73466 be included in the order as requested so that you will be able to confirm the specificity of the bands seen in your western blot. I am continuing to look for the first order you placed for ab15249 but I wanted to get 1 vial of the new antibody and the peptide to you as soon as possible so that you can continue with your experiments. If there is anything else I can do to help, please let me know.

    Read More

    Abcam Scientific Support

    Answered on Nov 08 2011

    1-10 of 17 Abreviews or Q&A

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