• Product name
    Anti-Wilms Tumor Protein antibody
    See all Wilms Tumor Protein primary antibodies
  • Description
    Rabbit polyclonal to Wilms Tumor Protein
  • Host species
  • Tested applications
    Suitable for: IHC-Fr, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide within Human Wilms Tumor Protein aa 400 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P19544



Our Abpromise guarantee covers the use of ab15249 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration. PubMed: 22586490
IHC-P 1/100.


  • Function
    Transcription factor that plays an important role in cellular development and cell survival. Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. Recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3'. It has a tumor suppressor as well as an oncogenic role in tumor formation. Function may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors. Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing. Isoform 1 has lower affinity for DNA, and can bind RNA.
  • Tissue specificity
    Expressed in the kidney and a subset of hematopoietic cells.
  • Involvement in disease
    Defects in WT1 are the cause of Frasier syndrome (FS) [MIM:136680]. FS is characterized by a slowly progressing nephropathy leading to renal failure in adolescence or early adulthood, male pseudohermaphroditism, and no Wilms tumor. As for histological findings of the kidneys, focal glomerular sclerosis is often observed. There is phenotypic overlap with Denys-Drash syndrome. Inheritance is autosomal dominant.
    Defects in WT1 are the cause of Wilms tumor 1 (WT1) [MIM:194070]. WT is an embryonal malignancy of the kidney that affects approximately 1 in 10'000 infants and young children. It occurs both in sporadic and hereditary forms.
    Defects in WT1 are the cause of Denys-Drash syndrome (DDS) [MIM:194080]. DDS is a typical nephropathy characterized by diffuse mesangial sclerosis, genital abnormalities, and/or Wilms tumor. There is phenotypic overlap with WAGR syndrome and Frasier syndrome. Inheritance is autosomal dominant, but most cases are sporadic.
    Defects in WT1 are the cause of nephrotic syndrome type 4 (NPHS4) [MIM:256370]. A renal disease characterized clinically by proteinuria, hypoalbuminemia, hyperlipidemia and edema. Kidney biopsies show non-specific histologic changes such as focal segmental glomerulosclerosis and diffuse mesangial proliferation. Some affected individuals have an inherited steroid-resistant form and progress to end-stage renal failure. Most patients with NPHS4 show diffuse mesangial sclerosis on renal biopsy, which is a pathologic entity characterized by mesangial matrix expansion with no mesangial hypercellularity, hypertrophy of the podocytes, vacuolized podocytes, thickened basement membranes, and diminished patency of the capillary lumen.
    Defects in WT1 are a cause of Meacham syndrome (MEACHS) [MIM:608978]. Meacham syndrome is a rare sporadically occurring multiple malformation syndrome characterized by male pseudohermaphroditism with abnormal internal female genitalia comprising a uterus and double or septate vagina, complex congenital heart defect and diaphragmatic abnormalities.
    Note=A chromosomal aberration involving WT1 may be a cause of desmoplastic small round cell tumor (DSRCT). Translocation t(11;22)(p13;q12) with EWSR1.
  • Sequence similarities
    Belongs to the EGR C2H2-type zinc-finger protein family.
    Contains 4 C2H2-type zinc fingers.
  • Cellular localization
    Nucleus. Cytoplasm. Shuttles between nucleus and cytoplasm; Nucleus > nucleoplasm and Nucleus speckle.
  • Information by UniProt
  • Database links
  • Alternative names
    • WIT 2 antibody
    • WT 1 antibody
    • AWT1 antibody
    • FWT1 antibody
    • GUD antibody
    • NPHS4 antibody
    • WAGR antibody
    • Wilms tumor 1 antibody
    • Wilms Tumor antibody
    • Wilms tumor protein antibody
    • Wilms' tumor gene antibody
    • Wilms' tumor protein antibody
    • WIT2 antibody
    • WT antibody
    • WT1 antibody
    • WT1_HUMAN antibody
    • WT33 antibody
    see all


  • Immunohistochemical analysis of murine kidney tissue, staining Wilms Tumor Protein (red) with ab15249 at 1/300 dilution. A fluorescent-conjugated secondary antibody was used to detect staining.


This product has been referenced in:

See all 18 Publications for this product

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
IHC - Wholemount
Mouse Embryo (lung, mesothelial cells)
lung, mesothelial cells

Abcam user community

Verified customer

Submitted Dec 14 2016

Abcam has not validated the combination of species/application used in this Abreview.
Immunohistochemistry (Frozen sections)
Mouse Tissue sections (lung, mesothelial cells)
lung, mesothelial cells
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Dec 14 2016

Thank you for your phone call today, and I apologize for the troublewith this antibody.

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Thank you for contacting us.

The exact immunogen sequence is confidential, but it is contained within the range between aa 389-449 of P19544 (Uniprot), isoform 1.


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I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement.
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Thank you for contacting Abcam.
For ab15429 we have found that there are problems with this antibody in western blot and that we can no longer guarantee that it will work in this application. However it is still guaranteed to work in IP and IHC-P....

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Thank you for your response. This is to let you know that I have confirmed and agreed on placing a new order for your customer - for one vial of ab96792 as a free of charge replacement. I hope the second vial will work as it is expected, and plea...

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Thank you for getting back to me and for clarifying the situation further. It is indeed the case that after consideration Western blot application has been removed from the datasheet. Although, you have purchased this antibody via an unauthorized...

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Thank you for your patience in this matter. I have processed the request for you to receive ab64681 as a replacement for ab15249 which did not work in western blot as stated on the datasheet. I have requested that the peptide ab73466 be included in the...

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