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    wilms-tumor-protein-antibody-can-r9ihc-56-2-ab89901.pdf

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Tags & Cell Markers Cell Type Markers Tumor Associated
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RecombinantRabMAb

Recombinant Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2] (ab89901)

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Reviews (41)Q&A (9)References (149)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2] (ab89901)
  • Immunocytochemistry/ Immunofluorescence - Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2] (ab89901)
  • Western blot - Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2] (ab89901)
  • Flow Cytometry (Intracellular) - Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2] (ab89901)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2] (ab89901)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2] (ab89901)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2] (ab89901)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2] (ab89901)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2] (ab89901)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2] (ab89901)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2] (ab89901)
  • Western blot - Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2] (ab89901)
  • Western blot - Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2] (ab89901)
  • Western blot - Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2] (ab89901)
  • Western blot - Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2] (ab89901)
  • Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2] (ab89901)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [CAN-R9(IHC)-56-2] to Wilms Tumor Protein
  • Suitable for: WB, IHC-P, Flow Cyt (Intra), ICC/IF
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

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Overview

  • Product name

    Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2]
    See all Wilms Tumor Protein primary antibodies
  • Description

    Rabbit monoclonal [CAN-R9(IHC)-56-2] to Wilms Tumor Protein
  • Host species

    Rabbit
  • Specificity

    Expression levels of the target protein vary with sample type and some optimisation may be required.
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt (Intra), ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment (GST-tag) within Human Wilms Tumor Protein aa 50-250. The exact sequence is proprietary.
    Database link: P19544
    (Peptide available as ab208102)

  • Positive control

    • WB: Human testis whole cell lysate. K562, Ramos, THP-1 and HeLa whole cell lysates. IHC-P: Human kidney, Wilms tumor, ovarian serous adenocarcinoma and fetal tissues; Rat and mouse testis tissues. ICC/IF: K562 cells. Flow Cyt (intra): K562 cells. IHC-Fr: Human embryonic kidney tissue.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    CAN-R9(IHC)-56-2
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Cell Type Markers
    • Tumor Associated
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Cancer
    • Tumor biomarkers
    • Other
    • Developmental Biology
    • Organogenesis
    • Excretory system development
    • Kidney development

Associated products

  • Alternative Versions

    • Alexa Fluor® 488 Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2] (ab202635)
    • Alexa Fluor® 647 Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2] (ab202639)
    • Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2] - BSA and Azide free (ab216646)
    • FITC Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2] (ab223934)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96899)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Ramos whole cell lysate (ab3955)
    • K-562 whole cell lysate (ab7911)
  • Recombinant Protein

    • Recombinant Human Wilms Tumor Protein (ab82233)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab89901 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (4)
1/500 - 1/1000. Predicted molecular weight: 55 kDa.

For unpurified use at 1/100.

IHC-P (14)
1/300. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/30.

Flow Cyt (Intra)
1/50.

For unpurified use at 1/3.  ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF (6)
1/50.

For unpurified use at 1/5.

Notes
WB
1/500 - 1/1000. Predicted molecular weight: 55 kDa.

For unpurified use at 1/100.

IHC-P
1/300. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/30.

Flow Cyt (Intra)
1/50.

For unpurified use at 1/3.  ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF
1/50.

For unpurified use at 1/5.

Application notes
Is unsuitable for IP.

Target

  • Function

    Transcription factor that plays an important role in cellular development and cell survival. Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. Recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3'. It has a tumor suppressor as well as an oncogenic role in tumor formation. Function may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors. Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing. Isoform 1 has lower affinity for DNA, and can bind RNA.
  • Tissue specificity

    Expressed in the kidney and a subset of hematopoietic cells.
  • Involvement in disease

    Defects in WT1 are the cause of Frasier syndrome (FS) [MIM:136680]. FS is characterized by a slowly progressing nephropathy leading to renal failure in adolescence or early adulthood, male pseudohermaphroditism, and no Wilms tumor. As for histological findings of the kidneys, focal glomerular sclerosis is often observed. There is phenotypic overlap with Denys-Drash syndrome. Inheritance is autosomal dominant.
    Defects in WT1 are the cause of Wilms tumor 1 (WT1) [MIM:194070]. WT is an embryonal malignancy of the kidney that affects approximately 1 in 10'000 infants and young children. It occurs both in sporadic and hereditary forms.
    Defects in WT1 are the cause of Denys-Drash syndrome (DDS) [MIM:194080]. DDS is a typical nephropathy characterized by diffuse mesangial sclerosis, genital abnormalities, and/or Wilms tumor. There is phenotypic overlap with WAGR syndrome and Frasier syndrome. Inheritance is autosomal dominant, but most cases are sporadic.
    Defects in WT1 are the cause of nephrotic syndrome type 4 (NPHS4) [MIM:256370]. A renal disease characterized clinically by proteinuria, hypoalbuminemia, hyperlipidemia and edema. Kidney biopsies show non-specific histologic changes such as focal segmental glomerulosclerosis and diffuse mesangial proliferation. Some affected individuals have an inherited steroid-resistant form and progress to end-stage renal failure. Most patients with NPHS4 show diffuse mesangial sclerosis on renal biopsy, which is a pathologic entity characterized by mesangial matrix expansion with no mesangial hypercellularity, hypertrophy of the podocytes, vacuolized podocytes, thickened basement membranes, and diminished patency of the capillary lumen.
    Defects in WT1 are a cause of Meacham syndrome (MEACHS) [MIM:608978]. Meacham syndrome is a rare sporadically occurring multiple malformation syndrome characterized by male pseudohermaphroditism with abnormal internal female genitalia comprising a uterus and double or septate vagina, complex congenital heart defect and diaphragmatic abnormalities.
    Note=A chromosomal aberration involving WT1 may be a cause of desmoplastic small round cell tumor (DSRCT). Translocation t(11;22)(p13;q12) with EWSR1.
  • Sequence similarities

    Belongs to the EGR C2H2-type zinc-finger protein family.
    Contains 4 C2H2-type zinc fingers.
  • Cellular localization

    Nucleus. Cytoplasm. Shuttles between nucleus and cytoplasm; Nucleus > nucleoplasm and Nucleus speckle.
  • Target information above from: UniProt accession P19544 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 7490 Human
    • Entrez Gene: 22431 Mouse
    • Omim: 607102 Human
    • SwissProt: P19544 Human
    • SwissProt: P22561 Mouse
    • Unigene: 591980 Human
    • Alternative names

      • WIT 2 antibody
      • WT 1 antibody
      • AWT1 antibody
      • FWT1 antibody
      • GUD antibody
      • NPHS4 antibody
      • WAGR antibody
      • Wilms tumor 1 antibody
      • Wilms Tumor antibody
      • Wilms tumor protein antibody
      • Wilms' tumor gene antibody
      • Wilms' tumor protein antibody
      • WIT2 antibody
      • WT antibody
      • WT1 antibody
      • WT1_HUMAN antibody
      • WT33 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2] (ab89901)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2] (ab89901)Adams et al PLoS Genet. 2018 Feb 20;14(2):e1007233. doi: 10.1371/journal.pgen.1007233. eCollection 2018 Feb. Fig S1. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

      Spermatogenic phenotypes of Rnf8;Scml2-dKO mice.

      Immunostaining of testicular sections with WT1 (ab89901), a marker of Sertoli cells, and PLZF, a marker of undifferentiated spermatogonia.

      For preparation of testicular paraffin blocks, testes of mutants and littermate controls were fixed with 4% paraformaldehyde at 4°C overnight. Testes were then dehydrated and embedded in paraffin. For histological analyses, 6 μm-thick paraffin sections were deparaffinized and autoclaved in Target Retrieval Solution at 121°C for 20 min. The sections were blocked with for 10 min at room temperature, and then incubated with primary antibodies at 4°C overnight.

    • Immunocytochemistry/ Immunofluorescence - Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2] (ab89901)
      Immunocytochemistry/ Immunofluorescence - Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2] (ab89901)

      Immunocytochemsitry/Immunofluorescence analysis of K562 (Human chronic myelogenous leukemia cell line from bone marrow) cells labeling Wilms Tumor Protein (green) with purified ab89901 at 1/50.

      Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/200) was used as the secondary antibody. Counterstained with DAPI (blue).

    • Western blot - Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2] (ab89901)
      Western blot - Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2] (ab89901)
      All lanes : Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2] (ab89901) at 1/500 dilution

      Lane 1 : Human testis whole cell lysate. at 15 µg
      Lane 2 : K562 (Human T cell leukemia T lymphocyte) whole cell lysates at 4 µg
      Lane 3 : Mouse testis whole cell lysates at 15 µg

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 55 kDa
      Observed band size: 55 kDa



      Exposure time: Left image: 3 minutes
                               Right image: 15 seconds

      This antibody shows low affinity in mouse sample.

      Blocking and diluting buffer: 5% NFDM/TBST

       

       

    • Flow Cytometry (Intracellular) - Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2] (ab89901)
      Flow Cytometry (Intracellular) - Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2] (ab89901)

      Intracellular Flow Cytometry analysis of K-562 (Human chronic myelogenous leukemia lymphoblast) cells labeling Wilms Tumor Protein with ab89901 at 1/200 dilution (0.1 µg)/ Red. 

      Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A Goat anti rabbit IgG (Alexa Fluorr® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Rabbit monoclonal IgG (ab172730) / Black was used as the isotype control. Cells without incubation with primary antibody and secondary antibody / Blue was used as the unlabeled control. 

       

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2] (ab89901)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2] (ab89901)Verma et al PLoS One. 2018 Jun 20;13(6):e0198013. doi: 10.1371/journal.pone.0198013. eCollection 2018. Fig S1. Reproduced under the Creative Commons license https://creativecommons.org/publicdomain/zero/1.0/

      Podocyte counting using WT1 staining.

      Mouse kidney sections were stained with WT1 antibody ab89901 using immunohistochemistry. (A) Each glomeruli was identified and numbered using ImagePro premier imaging software. (B) A tracing was applied along the Bowman’s capsule to assess the surface area of the glomerulus. (C) Each WT1 positive nucleus was identified and the nuclear size assessed.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2] (ab89901)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2] (ab89901)Image is courtesy of an anonymous Abreview.

      Immunohistochemical analysis of fromaldehyde fixed, paraffin embedded rat testis tissue sections, labeling Wilms Tumor Protein using ab89901.

      Heat mediated antigen retrival was performed using 10 mM sodium citrate and 0.05% Tween-20. Tissue sections were incubated with ab89901 at a 1/50 dilution for 12 hours at 4ºC. The tissues were blocked with 10% serum for 30 minutes at 25ºC. The secondary used was a Donkey CY3® conjugate at a 1/200 dilution.

      See Abreview

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2] (ab89901)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2] (ab89901)

      ab89901 staining Wilms Tumor Protein in paraffin-embedded human ovarian serous adenocarcinoma tissue sections by Immunohistochemistry.

      Antigen retrieval was by heat mediation using citrate buffer (pH 6.0) was performed. Samples were incubated with primary antibody at 1:500 dilution (0.49 μg/ml). A ready to use Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Nuclear staining on human ovarian serous adenocarcinoma (PMID: 11939727) is observed.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2] (ab89901)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2] (ab89901)

      ab89901 staining Wilms Tumor Protein in paraffin-embedded mouse testis tissue sections by Immunohistochemistry.

      Antigen retrieval was by heat mediation using citrate buffer (pH 6.0). Samples were incubated with primary antibody at 1:500 dilution (0.49 μg/ml). A ready to use Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Nuclear staining on Sertoli cells in mouse testis (PMID: 21863216) is observed.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2] (ab89901)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2] (ab89901)

      ab89901 staining Wilms Tumor Protein in paraffin-embedded human kidney tissue sections by Immunohistochemistry.

      Antigen retrieval was by heat mediation using citrate buffer (pH 6.0). Samples were incubated with primary antibody at 1:500 dilution (0.49 μg/ml). A ready to use Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Nuclear staining on human kidney glomerulus (PMID: 12898605) is observed.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2] (ab89901)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2] (ab89901)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human Wilms tumor tissue labeling Wilms Tumor Protein with unpurified ab89901 at 1/250.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2] (ab89901)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2] (ab89901)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human fetal tissue labeling Wilms Tumor Protein with unpurified ab89901 at 1/250.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Western blot - Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2] (ab89901)
      Western blot - Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2] (ab89901)
      Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2] (ab89901) at 1/100 dilution (unpurified) + K562 (Human chronic myelogenous leukemia cell line from bone marrow) cell lysate at 10 µg

      Secondary
      Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size: 55 kDa
      Observed band size: 55 kDa



      Blocking buffer and concentration: 5% NFDM/TBST.

    • Western blot - Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2] (ab89901)
      Western blot - Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2] (ab89901)
      All lanes : Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2] (ab89901) at 1/5000 dilution

      Lane 1 : K-562 (Human chronic myelogenous leukemia lymphoblast). Whole cell lysates
      Lane 2 : THP-1 (Human monocytic leukemia monocyte). Whole cell lysates
      Lane 3 : SH-SY5Y (Human neuroblastoma epithelial cell) Whole cell lysates
      Lane 4 : HeLa (Human cervix adenocarcinoma epithelial cell) Whole cell lysates

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 55 kDa


      Exposure time: 3 minutes


      WT1 expression varies in different cell lines. 

      Blocking and diluting buffer: 5% NFDM/TBST.

    • Western blot - Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2] (ab89901)
      Western blot - Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2] (ab89901)
      Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2] (ab89901) at 1/1000 dilution (purified) + K562 (Human chronic myelogenous leukemia cell line from bone marrow) cell lysate at 10 µg

      Secondary
      Peroxidase-conjugated goat anto-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size: 55 kDa
      Observed band size: 55 kDa



      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Western blot - Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2] (ab89901)
      Western blot - Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2] (ab89901)
      Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2] (ab89901) at 1/1000 dilution (unpurified) + Ramos (Human Burkitt's lymphoma cell line) cell lysate

      Predicted band size: 55 kDa

    • Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2] (ab89901)
      Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2] (ab89901)

    Protocols

    • Flow cytometry protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (149)

    Publishing research using ab89901? Please let us know so that we can cite the reference in this datasheet.

    ab89901 has been referenced in 149 publications.

    • Du A  et al. Protein expression pattern of calcium-responsive transactivator in early postnatal and adult testes. Histochem Cell Biol 155:491-502 (2021). PubMed: 33398438
    • Yang Z  et al. Plasma cells shape the mesenchymal identity of ovarian cancers through transfer of exosome-derived microRNAs. Sci Adv 7:N/A (2021). PubMed: 33627414
    • Cleal L  et al. Resolving the heterogeneity of diaphragmatic mesenchyme: a novel mouse model of congenital diaphragmatic hernia. Dis Model Mech 14:N/A (2021). PubMed: 33735101
    • Huang Y  et al. A Novel, Personalized Drug-Screening System for Platinum-Resistant Ovarian Cancer Patients: A Preliminary Clinical Report. Cancer Manag Res 13:2849-2867 (2021). PubMed: 33833569
    • Kim JW  et al. Human kidney organoids model the tacrolimus nephrotoxicity and elucidate the role of autophagy. Korean J Intern Med N/A:N/A (2021). PubMed: 32972120
    View all Publications for this product

    Customer reviews and Q&As

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    1-10 of 50 Abreviews or Q&A

    IHC - Wholemount abreview for Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    IHC - Wholemount
    Sample
    Mouse Embryo (E12.5 Mouse Embryo)
    Specification
    E12.5 Mouse Embryo
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Alain Chedotal

    Verified customer

    Submitted Jan 31 2022

    IHC - Wholemount abreview for Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    IHC - Wholemount
    Sample
    Rat Embryo (E13.5 RAT Embryo)
    Specification
    E13.5 RAT Embryo
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Alain Chedotal

    Verified customer

    Submitted Jan 31 2022

    Immunohistochemistry (Frozen sections) abreview for Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2]

    Below Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rat Tissue sections (Sagittal embryonic Rat sections (E13.5))
    Permeabilization
    Yes - Triton
    Specification
    Sagittal embryonic Rat sections (E13.5)
    Blocking step
    (agent) for 1 hour(s) and 0 minute(s) · Concentration: 0.25% · Temperature: 21°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Alain Chedotal

    Verified customer

    Submitted Aug 09 2021

    Immunohistochemistry (Frozen sections) abreview for Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Sagittal embryonic mouse sections (E12.5))
    Permeabilization
    Yes - Triton
    Specification
    Sagittal embryonic mouse sections (E12.5)
    Blocking step
    (agent) for 1 hour(s) and 0 minute(s) · Concentration: 0.25% · Temperature: 21°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Alain Chedotal

    Verified customer

    Submitted Aug 03 2021

    Immunohistochemistry (Frozen sections) abreview for Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (PCW9.0 Human embryonic gonad)
    Permeabilization
    Yes - Triton
    Specification
    PCW9.0 Human embryonic gonad
    Blocking step
    (agent) for 1 hour(s) and 0 minute(s) · Concentration: 0.25% · Temperature: 21°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Alain Chedotal

    Verified customer

    Submitted Aug 03 2021

    Western blot abreview for Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2]

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (B16 cells)
    Gel Running Conditions
    Reduced Denaturing (4-20% tris-glycin gel)
    Loading amount
    40 µg
    Specification
    B16 cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Nov 02 2020

    Western blot abreview for Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2]

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (U937 cells)
    Gel Running Conditions
    Reduced Denaturing (4-20% tris-glycin gel)
    Loading amount
    40 µg
    Specification
    U937 cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Nov 02 2020

    Western blot abreview for Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Heart)
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Loading amount
    30 µg
    Specification
    Heart
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Aug 14 2020

    Immunohistochemistry (Frozen sections) abreview for Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Heart)
    Permeabilization
    Yes - Tween-20
    Specification
    Heart
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Aug 10 2020

    Question

    Thank you very much for contacting us and letting us know about the trouble with the vial of this antibody.

    Thank you also for sending the details of your protocol and the images. I do have a couple of additional questions, so that I completely understand the situation:

    1) How long do you perform the heat-mediated antigen retrieval, and in what kind of buffer (citrate, EDTA, etc)?

    12 hours for the heat-mediated antigen retrieval in EDTA buffer

    2) Have you changed any details of your protocol between the old and new vials?

    NO

    3) What was the lot number of the vial that worked in the past?

    the old vial was used up and throw away so the lot number of the vial that worked in the past was lost.It was ordered in Nov,2011.

    We do not test this antibody with rat samplse, and it is not covered by our guarantee. However since it has worked for you in the past, I'd be happy to send you a replacement vial. Would you be able to submit an Abreview online, and we may be able to add rat to the list of our guaranteed species?

    I look forward to hearing from you. Please let me know if you would like to receive a replacment vial and I will send this to you promptly.

    Read More

    Abcam community

    Verified customer

    Asked on Oct 23 2012

    Answer

    Thank you very much for your reply and for sending the additional information.

    Could you please confirm whether you would like a replacement from a different lot, or a credit or refund? I will arrange this promptly.

    I look forward to hearing from you. If you need anything else from me, please don't hesitate to ask. Have a nice day.

    Read More

    Abcam Scientific Support

    Answered on Oct 23 2012

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