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Thank you very much for contacting us and letting us know about the trouble with the vial of this antibody.
Thank you also for sending the details of your protocol and the images. I do have a couple of additional questions, so that I completely understand the situation:
1) How long do you perform the heat-mediated antigen retrieval, and in what kind of buffer (citrate, EDTA, etc)?
12 hours for the heat-mediated antigen retrieval in EDTA buffer
2) Have you changed any details of your protocol between the old and new vials?
3) What was the lot number of the vial that worked in the past?
the old vial was used up and throw away so the lot number of the vial that worked in the past was lost.It was ordered in Nov,2011.
We do not test this antibody with rat samplse, and it is not covered by our guarantee. However since it has worked for you in the past, I'd be happy to send you a replacement vial. Would you be able to submit an Abreview online, and we may be able to add rat to the list of our guaranteed species?
I look forward to hearing from you. Please let me know if you would like to receive a replacment vial and I will send this to you promptly.
Asked on Oct 23 2012
Thank you very much for your reply and for sending the additional information.
Could you please confirm whether you would like a replacement from a different lot, or a credit or refund? I will arrange this promptly.
I look forward to hearing from you. If you need anything else from me, please don't hesitate to ask. Have a nice day.
Answered on Oct 23 2012