Immunohistochemistry (Frozen sections) abreview for Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2]

Excellent
Abreviews
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Human embryonic kidney)
Permeabilization
Yes - 0.25% Triton X
Specification
Human embryonic kidney
Blocking step
BSA and donkey antisera as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
Fixative
Formaldehyde

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000

Secondary antibody

Dilution
1/500
Name
Non-Abcam antibody was used: AF594 donkey anti rabbit
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594

Additional data

Additional Notes
WT1 in red, DAPI in white, and transmitted light also shown for contrast. WT1 expression in the kidney – expected in the nephron progenitors population, presumptive and mature podocytes and cells lining collecting duct (GUDMAP.org). The antibody shows strong nuclear signal in cells with expected WT1 expression. A good antibody working well in both mouse and human samples. Would recommend use for human and mouse samples. Concentrations tested: 1:100, 1:250, 1:500, 1:1000 Best concentration: 1:1000 Possible that antibody will be useful at lower concentrations.

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Submitted Apr 04 2016

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