Western blot abreview for Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2]

Good
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (Whole brain tissue lysate)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (3-8% Bis-Tris)
Loading amount
20 µg
Specification
Whole brain tissue lysate
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Block
Dilution
1/1000

Secondary antibody

Dilution
1/15000
Name
Non-Abcam antibody was used: Goat Anti-Rabbit IgG (H+L) to HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL+
Negative control
No negative controls run
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 55 kDa Non-specific: 25, 32 kDa
Positive control
Showed expected band at 55 kDa plus background bands at 25 and 32 kDa

Additional data

Additional Notes
PVDF membrane.

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Submitted Mar 29 2017

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