Immunohistochemistry (Frozen sections) abreview for Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2]

Good
Abreviews
Application
Immunohistochemistry (Frozen sections)
Sample
Pig Tissue sections (Heart)
Permeabilization
Yes - PBS + Triton 0.1%
Specification
Heart
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative
Paraformaldehyde

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS + 0.05% Tween
Dilution
1/50

Secondary antibody

Dilution
1/200
Name
Non-Abcam antibody was used: Alexa fluor
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488

Additional data

Abcam response
We would like to thank the reviewer for participating in our AbTrial program and for submitting this AbReview for this product. As the product had not yet been tested on this tissue type we were unsure what the result might be, and we appreciate the time and effort that the reviewer has put in to testing this product in this application. We encourage all researchers to contact us should they wish to test any of our products in untested species or applications, as these may qualify for our AbTrial testing program.

Davide Maselli

Verified customer

Submitted Aug 23 2018

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