Flow Cytometry abreview for Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2]

Excellent
Abreviews
Application
Flow Cytometry
Sample
Human Cell (Epicardial cell)
Permeabilization
Yes - Commercial Fixation/Permeabilization Solution Kit
Gating Strategy
WT1 positive cells
Specification
Epicardial cell
Preparation
Cell harvesting/tissue preparation method: Human iPSC-derived epicardial cells
Sample buffer: 2% FBS, 2mM EDTA in 1X PBS
Fixation
Commercial Fixation/Permeabilization Solution Kit

Other product details

Incubation time
45 minute(s) · Temperature: 4°C · Diluent: 2% FBS, 2mM EDTA in 1X PBS
Dilution
1/100

Secondary antibody

Dilution
1/200
Name
Non-Abcam antibody was used: Goat anti-Rabbit IgG (H+L) Secondary Antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594

Abcam user community

Verified customer

Submitted Apr 08 2019

Sign up