Key features and details
- Rabbit polyclonal to WIPI1
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-WIPI1 antibody
See all WIPI1 primary antibodies
DescriptionRabbit polyclonal to WIPI1
SpecificityAt least two isoforms of WIPI1 are known to exist; ab139722 will detect both isoforms.
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to 17 amino acid residues near the carboxy terminus of Human WIPI1 (NP_060453).
- Rat colon tissue lysate.
Storage instructionsShipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store undiluted.
Storage bufferPreservative: 0.02% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab139722 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 49 kDa.|
|ICC/IF||Use a concentration of 20 µg/ml.|
FunctionMay play a role in autophagy. May regulate the trafficking of proteins involved in the mannose-6-phosphate receptor (MPR) recycling pathway.
Tissue specificityUbiquitously expressed. Highly expressed in skeletal muscle, heart, testis, pancreas and placenta. Highly expressed in G361, Sk-mel-28, Sk-mel-13, WM852 and WM451 cells. Up-regulated in a variety of tumor tissues.
Sequence similaritiesBelongs to the WD repeat SVP1 family.
Contains 3 WD repeats.
Cellular localizationGolgi apparatus > trans-Golgi network. Endosome. Cytoplasmic vesicle > clathrin-coated vesicle. Trans elements of the Golgi and peripheral endosomes. Dynamically cycles through these compartments and is susceptible to conditions that modulate membrane flux. Enriched in clathrin-coated vesicles.
- Information by UniProt
- ATG 18 antibody
- ATG18 antibody
- Atg18 protein homolog antibody
Immunofluorescence of WIPI1 in Human colon tissue with ab139722 at 20 ug/mL.
Lane 1 : Anti-WIPI1 antibody (ab139722) at 1 µg/ml
Lane 2 : Anti-WIPI1 antibody (ab139722) at 2 µg/ml
Lane 1 : Rat colon tissue lysate
Lane 2 : Rat colon tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 49 kDa
ab139722 has not yet been referenced specifically in any publications.