Key features and details
- Rabbit polyclonal to WIPI1 - C-terminal
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-WIPI1 antibody - C-terminal
See all WIPI1 primary antibodies
DescriptionRabbit polyclonal to WIPI1 - C-terminal
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Pig
Recombinant fragment within Human WIPI1 (C terminal). The exact sequence is proprietary.
Database link: Q5MNZ9
- WB: HepG2 whole cell extract; Human WIPI1 transfected HEK-293T whole cell extract.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab227880 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/20000. Predicted molecular weight: 49 kDa.|
FunctionMay play a role in autophagy. May regulate the trafficking of proteins involved in the mannose-6-phosphate receptor (MPR) recycling pathway.
Tissue specificityUbiquitously expressed. Highly expressed in skeletal muscle, heart, testis, pancreas and placenta. Highly expressed in G361, Sk-mel-28, Sk-mel-13, WM852 and WM451 cells. Up-regulated in a variety of tumor tissues.
Sequence similaritiesBelongs to the WD repeat SVP1 family.
Contains 3 WD repeats.
Cellular localizationGolgi apparatus > trans-Golgi network. Endosome. Cytoplasmic vesicle > clathrin-coated vesicle. Trans elements of the Golgi and peripheral endosomes. Dynamically cycles through these compartments and is susceptible to conditions that modulate membrane flux. Enriched in clathrin-coated vesicles.
- Information by UniProt
- ATG 18 antibody
- ATG18 antibody
- Atg18 protein homolog antibody
Anti-WIPI1 antibody - C-terminal (ab227880) at 1/500 dilution + HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract at 30 µg
Predicted band size: 49 kDa
10 % SDS-PAGE gel.
All lanes : Anti-WIPI1 antibody - C-terminal (ab227880) at 1/20000 dilution
Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : Human WIPI1 transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 49 kDa
10% SDS-PAGE gel.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab227880 has not yet been referenced specifically in any publications.