Product nameAnti-WIPI1 antibody [EPR8110]
See all WIPI1 primary antibodies
DescriptionRabbit monoclonal [EPR8110] to WIPI1
Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human WIPI1 aa 50-150. The exact sequence is proprietary.
- A375, BxPC3, 293T, C6, RAW264.7, NIH 3T3, and JAR cell lysates; Human colon tissue.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 1.14 x 10 -10 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab128901 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionMay play a role in autophagy. May regulate the trafficking of proteins involved in the mannose-6-phosphate receptor (MPR) recycling pathway.
Tissue specificityUbiquitously expressed. Highly expressed in skeletal muscle, heart, testis, pancreas and placenta. Highly expressed in G361, Sk-mel-28, Sk-mel-13, WM852 and WM451 cells. Up-regulated in a variety of tumor tissues.
Sequence similaritiesBelongs to the WD repeat SVP1 family.
Contains 3 WD repeats.
Cellular localizationGolgi apparatus > trans-Golgi network. Endosome. Cytoplasmic vesicle > clathrin-coated vesicle. Trans elements of the Golgi and peripheral endosomes. Dynamically cycles through these compartments and is susceptible to conditions that modulate membrane flux. Enriched in clathrin-coated vesicles.
- Information by UniProt
- ATG 18 antibody
- ATG18 antibody
- Atg18 protein homolog antibody
All lanes : Anti-WIPI1 antibody [EPR8110] (ab128901) at 1/1000 dilution
Lane 1 : A375 cell lysate
Lane 2 : BxPC3 cell lysate
Lane 3 : 293T cell lysate
Lane 4 : C6 cell lysate
Lane 5 : RAW264.7 cell lysate
Lane 6 : NIH 3T3 cell lysate
Lane 7 : JAR cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 49 kDa
ab128901 at 1/50 dilution staining WIPI1 in Paraffin-embedded Human colon tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
This product has been referenced in:
- Chen K et al. Optineurin-mediated mitophagy protects renal tubular epithelial cells against accelerated senescence in diabetic nephropathy. Cell Death Dis 9:105 (2018). IF . Read more (PubMed: 29367621) »
- Chen K et al. Optineurin inhibits NLRP3 inflammasome activation by enhancing mitophagy of renal tubular cells in diabetic nephropathy. FASEB J N/A:fj201801749RRR (2018). Read more (PubMed: 30571313) »