Anti-WISP1 antibody - C-terminal (ab178547)
- Datasheet
- References (4)
- Protocols
Overview
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Product name
Anti-WISP1 antibody - C-terminal
See all WISP1 primary antibodies -
Description
Rabbit polyclonal to WISP1 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC, IHC-Fr, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human WISP1 aa 354-367 (C terminal). Differs from the related mouse and rat sequences by one amino acid.
Sequence:ADLESYPDFSEIAN
Database link: O95388 -
Positive control
- WB: Rat heart, kidney and lung tissue lysates; A549, A431 and COLO320 cell lysates. IHC-P: Rat kidney and pancreas tissue; Human placenta tissue ICC: MCF7 cells. IHC-Fr: Rat cardiac muscle tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab178547 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC | Use a concentration of 0.5 - 1 µg/ml. | |
| IHC-Fr | Use a concentration of 0.5 - 1 µg/ml. | |
| WB | Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 40 kDa. |
Target
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Relevance
WISP1 is a downstream regulator in the Wnt/Frizzled-signaling pathway and is associated with cell survival. WISP1 attentuates p53-mediated apoptosis in response to DNA damage through activation of AKT kinase and up-regulates the anti-apoptotic Bcl-X(L) protein. WISP1 is expressed in heart, kidney, lung, pancreas, placenta, ovary, small intestine and spleen. Overexpression is associated with several cancers including breast cancer and colon tumors. -
Cellular localization
Secreted -
Database links
- Entrez Gene: 8840 Human
- Entrez Gene: 22402 Mouse
- Entrez Gene: 65154 Rat
- Omim: 603398 Human
- SwissProt: O95388 Human
- SwissProt: O54775 Mouse
- SwissProt: Q99PP0 Rat
- Unigene: 492974 Human
see all -
Alternative names
- CCN 4 antibody
- CCN family member 4 antibody
- CCN4 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WISP1 antibody - C-terminal (ab178547)Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling WISP1 with ab178547 at 1 µg/ml.
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Western blot - Anti-WISP1 antibody - C-terminal (ab178547)All lanes : Anti-WISP1 antibody - C-terminal (ab178547) at 0.5 µg/ml
Lane 1 : Rat heart tissue lysate at 50 µg
Lane 2 : Rat kidney tissue lysate at 50 µg
Lane 3 : Rat lung tissue lysate at 50 µg
Lane 4 : A549 whole cell lysate at 40 µg
Lane 5 : A431 whole cell lysate at 40 µg
Lane 6 : COLO320 whole cell lysate at 40 µg
Predicted band size: 40 kDa
Observed band size: 40 kDa -
Immunohistochemistry (Frozen sections) - Anti-WISP1 antibody - C-terminal (ab178547)Immunohistochemical analysis of frozen rat cardiac muscle sections labeling WISP1 with ab178547 at 1μg/mL (incubated overnight at 4°C). Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WISP1 antibody - C-terminal (ab178547)Immunohistochemical analysis of paraffin-embedded rat pancreas tissue labeling WISP1 with ab178547 at 1 µg/ml.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WISP1 antibody - C-terminal (ab178547)Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling WISP1 with ab178547 at 1 µg/ml.
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Immunocytochemistry - Anti-WISP1 antibody - C-terminal (ab178547)Immunocytochemical analysis of MCF7 cells labeling WISP1 with ab178547 at 1 µg/ml.
Protocols
References
This product has been referenced in:
- Wu W et al. Tp53 Mutation Inhibits Ubiquitination and Degradation of WISP1 via Down-Regulation of Siah1 in Pancreatic Carcinogenesis. Front Pharmacol 9:857 (2018). WB, IHC-P ; Human . Read more (PubMed: 30123132) »
- Wu J et al. Early Detection of Urinary Proteome Biomarkers for Effective Early Treatment of Pulmonary Fibrosis in a Rat Model. Proteomics Clin Appl 11:N/A (2017). Read more (PubMed: 29028163) »
