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    wisp1-antibody-c-terminal-ab178547.pdf

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Anti-WISP1 antibody - C-terminal (ab178547)

  • Datasheet
  • SDS
Reviews (2) Submit a question References (9)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WISP1 antibody - C-terminal (ab178547)
  • Western blot - Anti-WISP1 antibody - C-terminal (ab178547)
  • Immunohistochemistry (Frozen sections) - Anti-WISP1 antibody - C-terminal (ab178547)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WISP1 antibody - C-terminal (ab178547)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WISP1 antibody - C-terminal (ab178547)
  • Immunocytochemistry - Anti-WISP1 antibody - C-terminal (ab178547)

Key features and details

  • Rabbit polyclonal to WISP1 - C-terminal
  • Suitable for: IHC-P, ICC, IHC-Fr, WB
  • Reacts with: Rat, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-WISP1 antibody - C-terminal
    See all WISP1 primary antibodies
  • Description

    Rabbit polyclonal to WISP1 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC, IHC-Fr, WBmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human WISP1 aa 354-367 (C terminal). Differs from the related mouse and rat sequences by one amino acid.
    Sequence:

    ADLESYPDFSEIAN


    Database link: O95388
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: Rat heart, kidney and lung tissue lysates; A549, A431 and COLO320 cell lysates. IHC-P: Rat kidney and pancreas tissue; Human placenta tissue ICC: MCF7 cells. IHC-Fr: Rat cardiac muscle tissue.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
    Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • DNA Damage Response
    • Other
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Developmental Families
    • Other
    • Stem Cells
    • Signaling Pathways
    • Wnt
    • Secreted

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • A-431 whole cell lysate (ab30132)
    • A-431 whole cell lysate (ab7909)
    • A549 whole cell lysate (ab7910)
  • Recombinant Protein

    • Recombinant human WISP1 protein (ab50041)
  • Related Products

    • Recombinant human WISP1 protein (ab50041)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab178547 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC
Use a concentration of 0.5 - 1 µg/ml.
IHC-Fr
Use a concentration of 0.5 - 1 µg/ml.
WB (2)
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 40 kDa.
Notes
IHC-P
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC
Use a concentration of 0.5 - 1 µg/ml.
IHC-Fr
Use a concentration of 0.5 - 1 µg/ml.
WB
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 40 kDa.

Target

  • Relevance

    WISP1 is a downstream regulator in the Wnt/Frizzled-signaling pathway and is associated with cell survival. WISP1 attentuates p53-mediated apoptosis in response to DNA damage through activation of AKT kinase and up-regulates the anti-apoptotic Bcl-X(L) protein. WISP1 is expressed in heart, kidney, lung, pancreas, placenta, ovary, small intestine and spleen. Overexpression is associated with several cancers including breast cancer and colon tumors.
  • Cellular localization

    Secreted
  • Database links

    • Entrez Gene: 8840 Human
    • Entrez Gene: 65154 Rat
    • Omim: 603398 Human
    • SwissProt: O95388 Human
    • SwissProt: Q99PP0 Rat
    • Unigene: 492974 Human
    • Unigene: 63486 Rat
    • Alternative names

      • CCN 4 antibody
      • CCN family member 4 antibody
      • CCN4 antibody
      • WISP 1 antibody
      • WISP 1c antibody
      • WISP 1i antibody
      • WISP 1tc antibody
      • WISP1c antibody
      • WISP1i antibody
      • WISP1tc antibody
      • WNT 1 induced secreted protein 1 antibody
      • Wnt 1 induced secreted protein antibody
      • WNT 1 inducible signaling pathway protein 1 antibody
      • Wnt 1 signaling pathway protein 1 antibody
      • Wnt-1 inducible signaling pathway protein 1 antibody
      • WNT1 induced secreted protein 1 antibody
      • Wnt1 induced secreted protein antibody
      • WNT1 inducible signaling pathway protein 1 antibody
      • Wnt1 signaling pathway protein 1 antibody
      • WNT1-inducible-signaling pathway protein 1 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WISP1 antibody - C-terminal (ab178547)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WISP1 antibody - C-terminal (ab178547)

      Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling WISP1 with ab178547 at 1 µg/ml.

    • Western blot - Anti-WISP1 antibody - C-terminal (ab178547)
      Western blot - Anti-WISP1 antibody - C-terminal (ab178547)
      All lanes : Anti-WISP1 antibody - C-terminal (ab178547) at 0.5 µg/ml

      Lane 1 : Rat heart tissue lysate at 50 µg
      Lane 2 : Rat kidney tissue lysate at 50 µg
      Lane 3 : Rat lung tissue lysate at 50 µg
      Lane 4 : A549 whole cell lysate at 40 µg
      Lane 5 : A431 whole cell lysate at 40 µg
      Lane 6 : COLO320 whole cell lysate at 40 µg

      Predicted band size: 40 kDa
      Observed band size: 40 kDa

    • Immunohistochemistry (Frozen sections) - Anti-WISP1 antibody - C-terminal (ab178547)
      Immunohistochemistry (Frozen sections) - Anti-WISP1 antibody - C-terminal (ab178547)
      Immunohistochemical analysis of frozen rat cardiac muscle sections labeling WISP1 with ab178547 at 1μg/mL (incubated overnight at 4°C). Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WISP1 antibody - C-terminal (ab178547)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WISP1 antibody - C-terminal (ab178547)

      Immunohistochemical analysis of paraffin-embedded rat pancreas tissue labeling WISP1 with ab178547 at 1 µg/ml.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WISP1 antibody - C-terminal (ab178547)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WISP1 antibody - C-terminal (ab178547)

      Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling WISP1 with ab178547 at 1 µg/ml.

    • Immunocytochemistry - Anti-WISP1 antibody - C-terminal (ab178547)
      Immunocytochemistry - Anti-WISP1 antibody - C-terminal (ab178547)

      Immunocytochemical analysis of MCF7 cells labeling WISP1 with ab178547 at 1 µg/ml.

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (9)

    Publishing research using ab178547? Please let us know so that we can cite the reference in this datasheet.

    ab178547 has been referenced in 9 publications.

    • Yu Z  et al. WISP1 and TLR4 on Macrophages Contribute to Ventilator-Induced Lung Injury. Inflammation 43:425-432 (2020). PubMed: 32130574
    • Schmoll D  et al. Activation of Adenosine Monophosphate-Activated Protein Kinase Reduces the Onset of Diet-Induced Hepatocellular Carcinoma in Mice. Hepatol Commun 4:1056-1072 (2020). PubMed: 32626837
    • Yang X  et al. WNT1-inducible signaling protein-1 mediates TGF-ß1-induced renal fibrosis in tubular epithelial cells and unilateral ureteral obstruction mouse models via autophagy. J Cell Physiol 235:2009-2022 (2020). PubMed: 31512238
    • Zhu J  et al. Down-regulation of long non-coding RNA SNHG14 protects against acute lung injury induced by lipopolysaccharide through microRNA-34c-3p-dependent inhibition of WISP1. Respir Res 20:233 (2019). PubMed: 31660971
    • Zhang LH  et al. Up-regulated Wnt1-inducible signaling pathway protein 1 correlates with poor prognosis and drug resistance by reducing DNA repair in gastric cancer. World J Gastroenterol 25:5814-5825 (2019). PubMed: 31636474
    • Wu W  et al. Tp53 Mutation Inhibits Ubiquitination and Degradation of WISP1 via Down-Regulation of Siah1 in Pancreatic Carcinogenesis. Front Pharmacol 9:857 (2018). WB, IHC-P ; Human . PubMed: 30123132
    • Wu J  et al. Early Detection of Urinary Proteome Biomarkers for Effective Early Treatment of Pulmonary Fibrosis in a Rat Model. Proteomics Clin Appl 11:N/A (2017). PubMed: 29028163
    • Murgai M  et al. KLF4-dependent perivascular cell plasticity mediates pre-metastatic niche formation and metastasis. Nat Med 23:1176-1190 (2017). PubMed: 28920957
    • Jin S  et al. MECHANICAL VENTILATION AUGMENTS POLY(I:C)INDUCED LUNG INJURY VIA A WISP1-INTEGRIN ß3 DEPENDENT PATHWAY IN MICE. Mol Med 22:54-63 (2016). PubMed: 26772774

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-2 of 2 Abreviews or Q&A

    Western blot abreview for Anti-WISP1 antibody - C-terminal

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (mouse hepatocytes)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Treatment
    100 ng/ml LPS 1 hour
    Specification
    mouse hepatocytes
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 09 2015

    Western blot abreview for Anti-WISP1 antibody - C-terminal

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    1e+006 cells
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Rat Cell lysate - whole cell (rat vascular smooth muscle cells)
    Specification
    rat vascular smooth muscle cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 27 2015

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