Recombinant Anti-WISP1 antibody [EPR22852-267] (ab260036)
![Western blot - Anti-WISP1 antibody [EPR22852-267] (ab260036)](https://www.abcam.com/ps/products/260/ab260036/Images/ab260036-355773-anti-wisp1-antibody-epr22852-267-western-blot-l6-rat.jpg "Western blot - Anti-WISP1 antibody [EPR22852-267] (ab260036)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22852-267] to WISP1
- Suitable for: IP, WB
- Reacts with: Mouse, Rat
Related conjugates and formulations
Overview
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Product name
Anti-WISP1 antibody [EPR22852-267]
See all WISP1 primary antibodies -
Description
Rabbit monoclonal [EPR22852-267] to WISP1 -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: B16-F10, C2C12, MEF, NIH/3T3 and L6 lysates. IP: C2C12 and L6 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22852-267 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab260036 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP |
1/30.
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| WB |
1/1000. Predicted molecular weight: 40 kDa.
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| Notes |
|---|
|
IP
1/30.
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WB
1/1000. Predicted molecular weight: 40 kDa. |
Target
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Relevance
WISP1 is a downstream regulator in the Wnt/Frizzled-signaling pathway and is associated with cell survival. WISP1 attentuates p53-mediated apoptosis in response to DNA damage through activation of AKT kinase and up-regulates the anti-apoptotic Bcl-X(L) protein. WISP1 is expressed in heart, kidney, lung, pancreas, placenta, ovary, small intestine and spleen. Overexpression is associated with several cancers including breast cancer and colon tumors. -
Cellular localization
Secreted -
Database links
- Entrez Gene: 22402 Mouse
- Entrez Gene: 65154 Rat
- SwissProt: O54775 Mouse
- SwissProt: Q99PP0 Rat
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Alternative names
- CCN 4 antibody
- CCN family member 4 antibody
- CCN4 antibody
see all
Images
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Western blot - Anti-WISP1 antibody [EPR22852-267] (ab260036)Anti-WISP1 antibody [EPR22852-267] (ab260036) at 1/1000 dilution + L6 (rat skeletal muscle myoblast) whole cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 40 kDa
Observed band size: 30,41 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST The expression profile and molecular weight observed is consistent with what has been described in the literature (PMID: 20010302, 30723155,17383426, 12064632), the 30kDa band is the splice variant of WISP1 as described in the literature (PMID: 17383426) Exposure time: 81 seconds.
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![Western blot - Anti-WISP1 antibody [EPR22852-267] (ab260036)](https://www.abcam.com/ps/products/260/ab260036/Images/ab260036-355772-anti-wisp1-antibody-epr22852-267-western-blot-mouse.jpg)
Western blot - Anti-WISP1 antibody [EPR22852-267] (ab260036)All lanes : Anti-WISP1 antibody [EPR22852-267] (ab260036) at 1/1000 dilution
Lane 1 : B16-F10 (mouse melanoma mixture of spindle-shaped and epithelial-like cells) whole cell lysate
Lane 2 : C2C12 (mouse myoblasts myoblast) whole cell lysate
Lane 3 : MEF (mouse embryonic fibroblast (immortalized)) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 40 kDa
Observed band size: 41 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST The expression profile and molecular weight observed is consistent with what has been described in the literature (PMID: 20010302, 30723155,17383426, 12064632), the 30kDa band is the splice variant of WISP1 as described in the literature (PMID: 17383426) Exposure time: Lane 1:37 seconds; Lane 2:59 seconds; Lanes 3: 3 minutes; Lane 4:15 seconds.
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![Immunoprecipitation - Anti-WISP1 antibody [EPR22852-267] (ab260036)](https://www.abcam.com/ps/products/260/ab260036/Images/ab260036-2-anti-wisp1-antibody-epr22852-267-immunoprecipitation-l6-rat.jpg)
Immunoprecipitation - Anti-WISP1 antibody [EPR22852-267] (ab260036)WISP1 was immunoprecipitated from 0.35 mg L6 (rat skeletal muscle myoblast) whole cell lysate with ab260036 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab260036 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.
Lane 1: L6 (rat skeletal muscle myoblast) whole cell lysate 10ug
Lane 2: ab260036 IP in L6 whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab260036 in L6 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 min
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![Immunoprecipitation - Anti-WISP1 antibody [EPR22852-267] (ab260036)](https://www.abcam.com/ps/products/260/ab260036/Images/ab260036-1-anti-wisp1-antibody-epr22852-267-immunoprecipitation-c2c12-mouse.jpg)
Immunoprecipitation - Anti-WISP1 antibody [EPR22852-267] (ab260036)WISP1 was immunoprecipitated from 0.35 mg C2C12 (mouse myoblasts myoblast) whole cell lysate with ab260036 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab260036 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.
Lane 1: C2C12 (mouse myoblasts myoblast) whole cell lysate 10ug
Lane 2: ab260036 IP in C2C12 whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab260036 in C2C12 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds.
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![Anti-WISP1 antibody [EPR22852-267] (ab260036)](https://www.abcam.com/ps/products/260/ab260036/Images/ab260036-5-benefits-of-recombinant-antibodies.png)
Anti-WISP1 antibody [EPR22852-267] (ab260036)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab260036 has not yet been referenced specifically in any publications.