Product nameAnti-WISP2 antibody
See all WISP2 primary antibodies
DescriptionRabbit polyclonal to WISP2
SpecificityThis antibody is specific for WISP2.
Tested applicationsSuitable for: IHC-P, ICC/IF, WB, ELISAmore details
Species reactivityReacts with: Human
A KLH conjugated synthetic peptide (10-30 aa in length) in the region of 127~141 of human WISP2.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPreservative: 0.09% Sodium azide
Concentration information loading...
PurityProtein G purified
Purification notesThis antibody is purified through a protein G column and eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS.
Our Abpromise guarantee covers the use of ab38317 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/25 - 1/100.|
|ICC/IF||Use a concentration of 1 - 5 µg/ml.|
|WB||1/1000. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).|
FunctionMay play an important role in modulating bone turnover. Promotes the adhesion of osteoblast cells and inhibits the binding of fibrinogen to integrin receptors. In addition, inhibits osteocalcin production.
Tissue specificityExpressed in primary osteoblasts, fibroblasts, ovary, testes, and heart.
Sequence similaritiesBelongs to the CCN family.
Contains 1 IGFBP N-terminal domain.
Contains 1 TSP type-1 domain.
Contains 1 VWFC domain.
- Information by UniProt
- CCN family member 5 antibody
- CCN5 antibody
- Connective tissue growth factor like protein antibody
Anti-WISP2 antibody (ab38317) at 1/1000 dilution + A549 whole cell lysate at 35 µg/ml
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size: 27 kDa
Observed band size: 27 kDa
Additional bands at: 50 kDa, 70 kDa. We are unsure as to the identity of these extra bands.
Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
ICC/IF image of ab38317 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab38317, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This product has been referenced in:
- Haque I et al. Leptin-induced ER-a-positive breast cancer cell viability and migration is mediated by suppressing CCN5-signaling via activating JAK/AKT/STAT-pathway. BMC Cancer 18:99 (2018). Read more (PubMed: 29370782) »
- Fiaturi N et al. CCN5 in alveolar epithelial proliferation and differentiation during neonatal lung oxygen injury. J Cell Commun Signal 12:217-229 (2018). Read more (PubMed: 29349730) »