Key features and details
- Rabbit polyclonal to WISP3
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-WISP3 antibody
See all WISP3 primary antibodies
DescriptionRabbit polyclonal to WISP3
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant fragment corresponding to Human WISP3 aa 201-372 (C terminal).
PLLQQLSTSYKTMPAYRNLPLIWKKKCLVQATKWTPCSRTCGMGISNRVT NENSNCEMRKEKRLCYIQPCDSNILKTIKIPKGKTCQPTFQLSKAEKFVF SGCSSTQSYKPTFCGICLDKRCCIPNKSKMITIQFDCPNEGSFKWKMLWI TSCVCQRNCREPGDIFSELKIL
Database link: O95389-2
- WB: HepG2 whole cell lysate (ab7900); Mouse heart, brain and kidney lysates; Rat heart and brain lysates. IHC-P: Human liver cancer and kidney tissues.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol, PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab224720 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|WB||1/500 - 1/5000. Detects a band of approximately 40 kDa (predicted molecular weight: 40, 42 kDa).|
FunctionAppears to be required for normal postnatal skeletal growth and cartilage homeostasis.
Tissue specificityPredominant expression in adult kidney and testis and fetal kidney. Weaker expression found in placenta, ovary, prostate and small intestine. Also expressed in skeletally-derived cells such as synoviocytes and articular cartilage chondrocytes.
Involvement in diseaseDefects in WISP3 are the cause of progressive pseudorheumatoid arthropathy of childhood (PPAC) [MIM:208230]. PPAC is an autosomal recessive disorder characterized by stiffness and swelling of joints, motor weakness and joint contractures. Signs and symptoms of the disease develop typically between three and eight years of age. This progressive disease is a primary disorder of articular cartilage with continued cartilage loss and destructive bone changes with aging.
Sequence similaritiesBelongs to the CCN family.
Contains 1 CTCK (C-terminal cystine knot-like) domain.
Contains 1 IGFBP N-terminal domain.
Contains 1 TSP type-1 domain.
- Information by UniProt
- CCN 6 antibody
- CCN family member 6 antibody
- CCN6 antibody
All lanes : Anti-WISP3 antibody (ab224720) at 1/500 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Lane 2 : Rat brain lysate
Lane 3 : Rat heart lysate
Lane 4 : Mouse heart lysate
Lane 5 : Mouse brain lysate
Lane 6 : Mouse kidney lysate
All lanes : Goat Anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 40, 42 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
Paraffin-embedded human kidney tissue stained for WISP3 using ab224720 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human liver cancer tissue stained for WISP3 using ab224720 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab224720 has been referenced in 2 publications.
- Yi Y et al. WISP3 prevents fibroblast-myofibroblast transdifferentiation in NRK-49F cells. Biomed Pharmacother 99:306-312 (2018). IHC-P . PubMed: 29353205
- McMullen ER et al. CCN6 regulates IGF2BP2 and HMGA2 signaling in metaplastic carcinomas of the breast. Breast Cancer Res Treat 172:577-586 (2018). PubMed: 30220054