Recombinant
RabMAb

Recombinant Anti-WISP3 antibody [EPR14769] - C-terminal (ab187666)

Overview

  • Product name

    Anti-WISP3 antibody [EPR14769] - C-terminal
    See all WISP3 primary antibodies
  • Description

    Rabbit monoclonal [EPR14769] to WISP3 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human WISP3 aa 300 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: O95389

  • Positive control

    • Caco-2, C6 and HepG2 whole cell lysate (ab7900); Mouse heart, mouse brain and rat brain lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab187666 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 39 kDa).

Target

  • Function

    Appears to be required for normal postnatal skeletal growth and cartilage homeostasis.
  • Tissue specificity

    Predominant expression in adult kidney and testis and fetal kidney. Weaker expression found in placenta, ovary, prostate and small intestine. Also expressed in skeletally-derived cells such as synoviocytes and articular cartilage chondrocytes.
  • Involvement in disease

    Defects in WISP3 are the cause of progressive pseudorheumatoid arthropathy of childhood (PPAC) [MIM:208230]. PPAC is an autosomal recessive disorder characterized by stiffness and swelling of joints, motor weakness and joint contractures. Signs and symptoms of the disease develop typically between three and eight years of age. This progressive disease is a primary disorder of articular cartilage with continued cartilage loss and destructive bone changes with aging.
  • Sequence similarities

    Belongs to the CCN family.
    Contains 1 CTCK (C-terminal cystine knot-like) domain.
    Contains 1 IGFBP N-terminal domain.
    Contains 1 TSP type-1 domain.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • CCN 6 antibody
    • CCN family member 6 antibody
    • CCN6 antibody
    • CYR61 antibody
    • LIBC antibody
    • Lost in inflammatory breast cancer tumor suppressor protein antibody
    • MGC125987 antibody
    • MGC125988 antibody
    • MGC125989 antibody
    • OTTHUMP00000040421 antibody
    • PPAC antibody
    • PPD antibody
    • UNQ462/PRO790/PRO956 antibody
    • WISP 3 antibody
    • WISP-3 antibody
    • WISP3 antibody
    • WISP3_HUMAN antibody
    • WNT 1 inducible signaling pathway protein 3 antibody
    • Wnt 1 signaling pathway protein 3 antibody
    • WNT1 inducible signaling pathway protein 3 antibody
    • WNT1 inducible signaling pathway protein 3 precursor antibody
    • WNT1-inducible-signaling pathway protein 3 antibody
    see all

Images

  • All lanes : Anti-WISP3 antibody [EPR14769] - C-terminal (ab187666) at 1/1000 dilution

    Lane 1 : Caco-2 cell lysate
    Lane 2 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 39 kDa
    Observed band size: 45 kDa
    why is the actual band size different from the predicted?

  • All lanes : Anti-WISP3 antibody [EPR14769] - C-terminal (ab187666) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Mouse heart lysate
    Lane 3 : Rat brain lysate
    Lane 4 : C6 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 39 kDa
    Observed band size: 45 kDa why is the actual band size different from the predicted?

References

This product has been referenced in:

  • McMullen ER  et al. CCN6 regulates IGF2BP2 and HMGA2 signaling in metaplastic carcinomas of the breast. Breast Cancer Res Treat 172:577-586 (2018). Read more (PubMed: 30220054) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Western blot
Sample
Pig Cell lysate - whole cell (Chondrocytes)
Gel Running Conditions
Reduced Denaturing (4-12%)
Specification
Chondrocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Oct 26 2016

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