- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-WldS antibody
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DescriptionRabbit polyclonal to WldS
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Host speciesRabbit
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Tested applicationsSuitable for: WB, ICC/IF, IHC-P, IHC-Fr, IPmore details
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Species reactivityReacts with: Mouse
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Immunogen
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
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Storage bufferPreservative: None
Constituents: Whole serum -
PurityWhole antiserum
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab94345 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | ||
| ICC/IF | ||
| IHC-P | ||
| IHC-Fr | ||
| IP |
Application notes
ICC/IF: 1/2000 - 1/5000. Specificity is shown by a clear signal in WldS cells which is absent in wild-type cells (as wild-types lack WldS).
IHC-P: 1/2000 - 1/5000. Specificity is shown by a clear signal in WldS cells which is absent in wild-type cells (as wild-types lack WldS).
IHC-Fr: 1/2000 - 1/5000. Specificity is shown by a clear signal in WldS cells which is absent in wild-type cells (as wild-types lack WldS).
IP: 1/20. See Laser, Conforti et al., 2006.
WB: 1/2000 - 1/5000. A single band is seen at these dilutions. Predicted molecular weight: 43 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/2000 - 1/5000. Specificity is shown by a clear signal in WldS cells which is absent in wild-type cells (as wild-types lack WldS).
IHC-Fr: 1/2000 - 1/5000. Specificity is shown by a clear signal in WldS cells which is absent in wild-type cells (as wild-types lack WldS).
IP: 1/20. See Laser, Conforti et al., 2006.
WB: 1/2000 - 1/5000. A single band is seen at these dilutions. Predicted molecular weight: 43 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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RelevanceThe slow Wallerian Degeneration (WldS) gene encodes a novel Ube4b-Nmnat1 chimeric protein (WldS protein). WldS protein confers a neuroprotective effect on axonal and synaptic compartments of neurons from a wide variety of degeneration-inducing stimuli. The chimeric WldS gene occurred as the result of a spontaneous mutation in C57BL/6 mice, resulting in a tandem triplication of a region already present on the distal region of chromosome 4. The triplicated region contains sequences coding for Nmnat1, Rbp7 and Ube4b. The boundaries within the triplicated region result in 2 copies of a fusion gene comprising the N70 terminal amino acids of Ube4b and the entire coding region of Nmnat1 (C Terminal 285 amino acids), linked by 18 amino acids from the 5' untranslated region of Nmnat1 which are not normally expressed.
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Cellular localizationNuclear
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Database links
- Entrez Gene: 230628 Mouse
- Entrez Gene: 66454 Mouse
- SwissProt: Q9EPA7 Mouse
- SwissProt: Q9EPC5 Mouse
- SwissProt: Q9ES00 Mouse
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Alternative names
- slow wallerian degeneration protein antibody
- Wallerian degeneration protein antibody
- Wld antibody
Images
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Western blot - Anti-WldS antibody (ab94345)All lanes : Anti-WldS antibody (ab94345) at 1/2000 dilution
Lane 1 : Wild-type mouse brain homogenate
Lane 2 : WldS mouse brain homogenate
Predicted band size: 43 kDa
Observed band size: 43 kDa
Protocols
References
ab94345 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"