Anti-WldS antibody (ab94345)
Key features and details
- Rabbit polyclonal to WldS
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-WldS antibody -
Description
Rabbit polyclonal to WldS -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Constituent: Whole serum -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab94345 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
| Notes |
|---|
IHC-P: 1/2000 - 1/5000. Specificity is shown by a clear signal in WldS cells which is absent in wild-type cells (as wild-types lack WldS).
IHC-Fr: 1/2000 - 1/5000. Specificity is shown by a clear signal in WldS cells which is absent in wild-type cells (as wild-types lack WldS).
IP: 1/20. See Laser, Conforti et al., 2006.
WB: 1/2000 - 1/5000. A single band is seen at these dilutions. Predicted molecular weight: 43 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Relevance
The slow Wallerian Degeneration (WldS) gene encodes a novel Ube4b-Nmnat1 chimeric protein (WldS protein). WldS protein confers a neuroprotective effect on axonal and synaptic compartments of neurons from a wide variety of degeneration-inducing stimuli. The chimeric WldS gene occurred as the result of a spontaneous mutation in C57BL/6 mice, resulting in a tandem triplication of a region already present on the distal region of chromosome 4. The triplicated region contains sequences coding for Nmnat1, Rbp7 and Ube4b. The boundaries within the triplicated region result in 2 copies of a fusion gene comprising the N70 terminal amino acids of Ube4b and the entire coding region of Nmnat1 (C Terminal 285 amino acids), linked by 18 amino acids from the 5' untranslated region of Nmnat1 which are not normally expressed. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 230628 Mouse
- Entrez Gene: 66454 Mouse
- SwissProt: Q9EPA7 Mouse
- SwissProt: Q9EPC5 Mouse
- SwissProt: Q9ES00 Mouse
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Alternative names
- slow wallerian degeneration protein antibody
- Wallerian degeneration protein antibody
- Wld antibody
Images
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Western blot - Anti-WldS antibody (ab94345)All lanes : Anti-WldS antibody (ab94345) at 1/2000 dilution
Lane 1 : Wild-type mouse brain homogenate
Lane 2 : WldS mouse brain homogenate
Predicted band size: 43 kDa
Observed band size: 43 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab94345 has been referenced in 1 publication.
- Mayoral SR et al. Initiation of CNS Myelination in the Optic Nerve Is Dependent on Axon Caliber. Cell Rep 25:544-550.e3 (2018). PubMed: 30332636