Anti-WNK1 antibody (ab233266)
Key features and details
- Rabbit polyclonal to WNK1
- Suitable for: WB, IHC-P
- Reacts with: Human, Pig
- Isotype: IgG
Overview
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Product name
Anti-WNK1 antibody
See all WNK1 primary antibodies -
Description
Rabbit polyclonal to WNK1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human, Pig
Predicted to work with: Mouse -
Immunogen
Recombinant fragment (His-T7-tag) corresponding to Human WNK1 aa 1950-2250. Expressed in E. coli. N-terminal tags.
Database link: Q9H4A3 -
Positive control
- WB: Recombinant human WNK1 protein. HEK-293T cell lysate. Pig liver and kidney tissue lysate. IHC-P: Human testis tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 44.12% PBS, 55.77% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab233266 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 - 5 µg/ml.
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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Notes |
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WB
Use a concentration of 1 - 5 µg/ml. |
IHC-P
Use a concentration of 5 - 20 µg/ml. |
Target
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Function
Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival, and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SCNN1A, SCNN1B, SCNN1D and SGK1. Controls sodium and chloride ion transport by inhibiting the activity of WNK4, by either phosphorylating the kinase or via an interaction between WNK4 and the autoinhibitory domain of WNK1. WNK4 regulates the activity of the thiazide-sensitive Na-Cl cotransporter, SLC12A3, by phosphorylation. WNK1 may also play a role in actin cytoskeletal reorganization. Phosphorylates NEDD4L. Acts as a scaffold to inhibit SLC4A4, SLC26A6 as well as CFTR activities and surface expression, recruits STK39 which mediates the inhibition. -
Tissue specificity
Widely expressed, with highest levels observed in the testis, heart, kidney and skeletal muscle. Isoform 3 is kidney-specific and specifically expressed in the distal convoluted tubule (DCT) and connecting tubule (CNT) of the nephron. -
Involvement in disease
Pseudohypoaldosteronism 2C
Neuropathy, hereditary sensory and autonomic, 2A -
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WNK subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsO-glycosylated.
Ubiquitinated in vitro by the BCR(KLHL3) complex and in vivo by a BCR(KLHL2) complex, leading to proteasomal degradation. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 65125 Human
- Entrez Gene: 232341 Mouse
- Entrez Gene: 406235 Pig
- Omim: 605232 Human
- SwissProt: Q9H4A3 Human
- SwissProt: P83741 Mouse
- SwissProt: Q6R2V0 Pig
- Unigene: 744906 Human
see all -
Alternative names
- Erythrocyte 65 kDa protein antibody
- HSAN2 antibody
- HSN2 antibody
see all
Images
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Formalin-fixed, paraffin-embedded human testis tissue stained for WNK1 with ab233266 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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All lanes : Anti-WNK1 antibody (ab233266) at 2 µg/ml
Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : Pig liver tissue lysate
Lane 3 : Pig kidney tissue lysate
Secondary
All lanes : HRP-linked Guinea pig anti-Rabbit at 1/2000 dilution -
Anti-WNK1 antibody (ab233266) at 2 µg/ml + Recombinant human WNK1 protein
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab233266 has not yet been referenced specifically in any publications.