Validated using a knockout cell line
Recombinant
RabMAb

Anti-WNK1 antibody [EPR2743(3)] (ab174854)

Overview

  • Product name
    Anti-WNK1 antibody [EPR2743(3)]
    See all WNK1 primary antibodies
  • Description
    Rabbit monoclonal [EPR2743(3)] to WNK1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, Flow Cytmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human WNK1 aa 100-200 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q9H4A3

  • Positive control
    • 293T and Human fetal brain lysates; 293T cells.
  • General notes

    This antibody was developed as part of a collaboration between Epitomics and the lab of Melanie Cobb at UT Southwestern Medical Center.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 50% Glycerol, 0.05% BSA, 49% PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR2743(3)
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab174854 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 250 kDa.
ICC/IF 1/100 - 1/250.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function
      Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival, and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SCNN1A, SCNN1B, SCNN1D and SGK1. Controls sodium and chloride ion transport by inhibiting the activity of WNK4, by either phosphorylating the kinase or via an interaction between WNK4 and the autoinhibitory domain of WNK1. WNK4 regulates the activity of the thiazide-sensitive Na-Cl cotransporter, SLC12A3, by phosphorylation. WNK1 may also play a role in actin cytoskeletal reorganization. Phosphorylates NEDD4L. Acts as a scaffold to inhibit SLC4A4, SLC26A6 as well as CFTR activities and surface expression, recruits STK39 which mediates the inhibition.
    • Tissue specificity
      Widely expressed, with highest levels observed in the testis, heart, kidney and skeletal muscle. Isoform 3 is kidney-specific and specifically expressed in the distal convoluted tubule (DCT) and connecting tubule (CNT) of the nephron.
    • Involvement in disease
      Pseudohypoaldosteronism 2C
      Neuropathy, hereditary sensory and autonomic, 2A
    • Sequence similarities
      Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WNK subfamily.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications
      O-glycosylated.
      Ubiquitinated in vitro by the BCR(KLHL3) complex and in vivo by a BCR(KLHL2) complex, leading to proteasomal degradation.
    • Cellular localization
      Cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • Erythrocyte 65 kDa protein antibody
      • HSAN2 antibody
      • HSN2 antibody
      • hWNK1 antibody
      • KDP antibody
      • KIAA0344 antibody
      • Kinase deficient protein antibody
      • MGC163339 antibody
      • MGC163341 antibody
      • p65 antibody
      • PPP1R167 antibody
      • PRKWNK1 antibody
      • Prostate derived sterile 20 like kinase antibody
      • Protein kinase lysine deficient 1 antibody
      • Protein kinase lysine-deficient 1 antibody
      • Protein kinase with no lysine 1 antibody
      • Protein phosphatase 1, regulatory subunit 167 antibody
      • PSK antibody
      • Serine/threonine protein kinase WNK1 1 antibody
      • Serine/threonine protein kinase WNK1 2 antibody
      • Serine/threonine protein kinase WNK1 antibody
      • Serine/threonine-protein kinase WNK1 antibody
      • With no K antibody
      • WNK lysine deficient protein kinase 1 antibody
      • WNK lysine deficient protein kinase 1 isoform antibody
      • WNK1 antibody
      • WNK1_HUMAN antibody
      see all

    Images

    • Lane 1: Wild type HAP1 whole cell lysate (40 µg)
      Lane 2: WNK1 knockout HAP1 whole cell lysate (40 µg)

      Lanes 1 - 2: Merged signal (red and green). Green - ab174854 observed at 300 kDa. Red - loading control, ab18058, observed at 130 kDa.

      ab174854 was shown to recognize WNK1 when WNK1 knockout samples were used, along with additional cross-reactive bands. Wild-type and WNK1 knockout samples were subjected to SDS-PAGE. Ab174854 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • Immunofluorescent analysis of 293T cells labeling WNK1 with ab174854 at 1/100 dilution (red). Nuclear DAPI staining (blue).

    • All lanes : Anti-WNK1 antibody [EPR2743(3)] (ab174854) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : Human fetal brain cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 250 kDa

    • Flow cytometric analysis of permeabilized 293T cells labeling WNK1 with ab174854 at 1/10 dilution (red), or a rabbit IgG (negative) (green).

    References

    ab174854 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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