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Synthetic peptide within Human WNK1 aa 100-200 (Cysteine residue). The exact sequence is proprietary.
Database link: Q9H4A3
This antibody was developed as part of a collaboration between Epitomics and the lab of Melanie Cobb at UT Southwestern Medical Center.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Our Abpromise guarantee covers the use of ab174854 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 250 kDa.|
|ICC/IF||1/100 - 1/250.|
|Flow Cyt||1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Lane 1: Wild type HAP1 whole cell lysate (40 µg)
Lane 2: WNK1 knockout HAP1 whole cell lysate (40 µg)
Lanes 1 - 2: Merged signal (red and green). Green - ab174854 observed at 300 kDa. Red - loading control, ab18058, observed at 130 kDa.
ab174854 was shown to recognize WNK1 when WNK1 knockout samples were used, along with additional cross-reactive bands. Wild-type and WNK1 knockout samples were subjected to SDS-PAGE. Ab174854 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
Immunofluorescent analysis of 293T cells labeling WNK1 with ab174854 at 1/100 dilution (red). Nuclear DAPI staining (blue).
Flow cytometric analysis of permeabilized 293T cells labeling WNK1 with ab174854 at 1/10 dilution (red), or a rabbit IgG (negative) (green).
ab174854 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"