Key features and details
- Rabbit polyclonal to WNK1 (phospho T58)
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-WNK1 (phospho T58) antibody
See all WNK1 primary antibodies
DescriptionRabbit polyclonal to WNK1 (phospho T58)
Specificityab53137 detects endogenous levels of WNK1 only when phosphorylated at threonine 58.
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
A synthetic phosphopeptide derived from human WNK1 around the phosphorylation site of threonine 58 (R-H-TP-M-D).
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab53137 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Our Abpromise guarantee covers the use of ab53137 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/300 - 1/1000. Detects a band of approximately 251 kDa (predicted molecular weight: 251 kDa).|
|IHC-P||Use at an assay dependent concentration.|
FunctionSerine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival, and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SCNN1A, SCNN1B, SCNN1D and SGK1. Controls sodium and chloride ion transport by inhibiting the activity of WNK4, by either phosphorylating the kinase or via an interaction between WNK4 and the autoinhibitory domain of WNK1. WNK4 regulates the activity of the thiazide-sensitive Na-Cl cotransporter, SLC12A3, by phosphorylation. WNK1 may also play a role in actin cytoskeletal reorganization. Phosphorylates NEDD4L. Acts as a scaffold to inhibit SLC4A4, SLC26A6 as well as CFTR activities and surface expression, recruits STK39 which mediates the inhibition.
Tissue specificityWidely expressed, with highest levels observed in the testis, heart, kidney and skeletal muscle. Isoform 3 is kidney-specific and specifically expressed in the distal convoluted tubule (DCT) and connecting tubule (CNT) of the nephron.
Involvement in diseasePseudohypoaldosteronism 2C
Neuropathy, hereditary sensory and autonomic, 2A
Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. WNK subfamily.
Contains 1 protein kinase domain.
Ubiquitinated in vitro by the BCR(KLHL3) complex and in vivo by a BCR(KLHL2) complex, leading to proteasomal degradation.
- Information by UniProt
- Erythrocyte 65 kDa protein antibody
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All lanes : Anti-WNK1 (phospho T58) antibody (ab53137) at 1/300 dilution
Lane 1 : 293 cell extract treated with EGF (200ng/ml, 30mins)
Lane 2 : 293 cell extract treated with EGF (200ng/ml, 30mins) with immunising peptide
Predicted band size: 251 kDa
ab53137 at 1/50 dilution staining WNK1 in human breast carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.
ab53137 has been referenced in 2 publications.
- Marshall WS et al. WNK1 and p38-MAPK distribution in ionocytes and accessory cells of euryhaline teleost fish implies ionoregulatory function. Biol Open 6:956-966 (2017). PubMed: 28522431
- Cheng CJ & Huang CL Activation of PI3-Kinase Stimulates Endocytosis of ROMK via Akt1/SGK1-Dependent Phosphorylation of WNK1. J Am Soc Nephrol 22:460-71 (2011). WB ; Human . PubMed: 21355052