Overview

  • Product name

    Anti-WNK4 antibody
  • Description

    Rabbit polyclonal to WNK4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    antigen sequence corresponding to amino acids 1005-1138 of Human WNK4.

  • Positive control

    • Human kidney tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab126477 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SCNN1A, SCNN1B, SCNN1D, SGK1, TRPV5 and TRPV6. Regulates the activity of the thiazide-sensitive Na-Cl cotransporter, SLC12A3, by phosphorylation which appears to prevent membrane trafficking of SLC12A3. Also inhibits the renal K(+) channel, KCNJ1, via a kinase-independent mechanism by which it induces clearance of the protein from the cell surface by clathrin-dependent endocytosis. WNK4 appears to act as a molecular switch that can vary the balance between NaCl reabsorption and K(+) secretion to maintain integrated homeostasis. Phosphorylates NEDD4L. Acts as a scaffold to inhibit SLC4A4 as well as CFTR activities and surface expression, recruits STK39 which mediates the inhibition.
  • Tissue specificity

    Expressed in kidney, colon and skin.
  • Involvement in disease

    Pseudohypoaldosteronism 2B
  • Sequence similarities

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WNK subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Phosphorylated by WNK1 and WNK3.
    Ubiquitinated by the BCR(KLHL3) complex, leading to its degradation and increased expression of KCNJ1 at the cell surface. Ubiquitinated by the BCR(KLHL2) complex.
  • Cellular localization

    Cell junction, tight junction. Present exclusively in intercellular junctions in the distal convoluted tubule and in both the cytoplasm and intercellular junctions in the cortical collecting duct. WNK4 is part of the tight junction complex (By similarity).
  • Information by UniProt
  • Database links

  • Alternative names

    • PHA 2B antibody
    • PHA2B antibody
    • PRKWNK 4 antibody
    • PRKWNK4 antibody
    • Protein kinase lysine deficient 4 antibody
    • Protein kinase lysine-deficient 4 antibody
    • Protein kinase with no lysine 4 antibody
    • Serine/threonine protein kinase WNK4 antibody
    • Serine/threonine-protein kinase WNK4 antibody
    • WNK lysine deficient protein kinase 4 antibody
    • Wnk4 antibody
    • WNK4_HUMAN antibody
    see all

Images

  • ab126477, at 1/20 dilution, staining WNK4 in paraffin-embedded Human kidney tissue by Immunohistochemistry.

References

This product has been referenced in:

  • Wolley MJ  et al. In Primary Aldosteronism, Mineralocorticoids Influence Exosomal Sodium-Chloride Cotransporter Abundance. J Am Soc Nephrol 28:56-63 (2017). Human . Read more (PubMed: 27381844) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Question
Answer

Thank you for calling Abcam earlier.

The testing discount details for ab126477 are below along with the new lab discount:

https://www.abcam.com/index.html?pageconfig=resource&rid=14561

TESTING DISCOUNT

DISCOUNT CODE: ******
Expiration date: **********

I am very pleased to hear you would like to accept our offer and test ab126477 in rat tissue. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for rat tissueand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Question
Answer

Thank you very much for your interest in ab126477.

To our knowledge, ab126477 has not been tested in mouse tissue. Therefore, I can offer a discount off a future purchase if you buy ab126477 now, test it in mouse tissue and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free PRIMARY ANTIBODY.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab126477 in mouse tissue. I will then send a discount code. This code must be issued before purchasing ab126477 so please wait for my reply before ordering.

2. Purchase ab126477 either by phone, fax, or online (www.abcam.com).

3. Test it in mouse tissue.
4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any PRIMARY ANTIBODY ordered and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab126477 turns out to be unsuitable for mouse tissue, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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