Product nameAnti-Wnt1 antibody
See all Wnt1 primary antibodies
DescriptionRabbit polyclonal to Wnt1
Tested applicationsSuitable for: ELISA, ICC/IF, IHC-P, IHC-Fr, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
- Breast carcinoma. NIH/3T3 cell lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.1% Sodium azide
Constituents: 0.0268% PBS, 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab15251 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
|ICC/IF||Use a concentration of 1 µg/ml.|
|IHC-Fr||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 41 kDa.|
FunctionLigand for members of the frizzled family of seven transmembrane receptors. In some developmental processes, is also a ligand for the coreceptor RYK, thus triggering Wnt signaling. Probable developmental protein. May be a signaling molecule important in CNS development. Is likely to signal over only few cell diameters.
Sequence similaritiesBelongs to the Wnt family.
Cellular localizationSecreted > extracellular space > extracellular matrix.
- Information by UniProt
- BMND16 antibody
- INT1 antibody
- OI15 antibody
Anti-Wnt1 antibody (ab15251) at 1/25 dilution + NIH3T3 cell lysate
Predicted band size: 41 kDa
Observed band size: 41 kDa
ab15251 staining Wnt1 in breast carcinoma by Immunohistochemistry (FFPE-sections).
ICC/IF image of ab15251 stained mouse 3T3 cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab15251, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).
Breast carcinoma cells stained with ab15251 (Immunohistochemistry, paraffin sections);
This product has been referenced in:
- Linde N et al. Macrophages orchestrate breast cancer early dissemination and metastasis. Nat Commun 9:21 (2018). Read more (PubMed: 29295986) »
- Li X et al. Tunicamycin inhibits progression of glioma cells through downregulation of the MEG-3-regulated wnt/ß-catenin signaling pathway. Oncol Lett 15:8470-8476 (2018). Read more (PubMed: 29805584) »