Key features and details
- Rabbit polyclonal to Wnt1 (Biotin)
- Suitable for: Sandwich ELISA, WB
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG
Product nameAnti-Wnt1 antibody (Biotin)
See all Wnt1 primary antibodies
DescriptionRabbit polyclonal to Wnt1 (Biotin)
Tested applicationsSuitable for: Sandwich ELISA, WBmore details
Species reactivityReacts with: Human
Highly pure (>98%) recombinant Humna Wnt1.
FormLyophilized:Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab84243 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use a concentration of 0.25 - 1 µg/ml.
Detects at least 0.2 – 0.4 ng/well of recombinant human Wnt1.
|WB||Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 41 kDa.
The detection limit for recombinant human Wnt1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
FunctionLigand for members of the frizzled family of seven transmembrane receptors. In some developmental processes, is also a ligand for the coreceptor RYK, thus triggering Wnt signaling. Probable developmental protein. May be a signaling molecule important in CNS development. Is likely to signal over only few cell diameters.
Sequence similaritiesBelongs to the Wnt family.
Cellular localizationSecreted > extracellular space > extracellular matrix.
- Information by UniProt
- BMND16 antibody
- INT1 antibody
- OI15 antibody
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab84243 has not yet been referenced specifically in any publications.