Key features and details
- Rabbit polyclonal to Wnt10a
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-Wnt10a antibody
See all Wnt10a primary antibodies
DescriptionRabbit polyclonal to Wnt10a
SpecificityDespite the high homology of Wnt10a to Wnt10b, ab106522 will not cross-react with Wnt10b.
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
A 14 amino acid synthetic peptide from near the C terminus of Human Wnt10a (UniProt Q9GZT5).
- WB: RAW264.7 cell lysate. IHC-P: Human and mouse skeletal muscle tissue; Rat bladder tissue. ICC/IF: Human skeletal muscle cells.
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We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at 4°C (stable for up to 12 months).
Storage bufferpH: 7.2
Preservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab106522 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 20 µg/ml.|
|WB||Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 46 kDa.|
|IHC-P||Use at an assay dependent concentration.|
FunctionLigand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule important in CNS development. Is likely to signal over only few cell diameters.
Involvement in diseaseDefects in WNT10A are a cause of ectodermal dysplasia anhidrotic (EDA) [MIM:224900]; also known ectodermal dysplasia hypohidrotic autosomal recessive (HED). Ectodermal dysplasia defines a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. EDA is characterized by sparse hair (atrichosis or hypotrichosis), abnormal or missing teeth and the inability to sweat due to the absence of sweat glands. Note=Most patients carrying WNT10A mutations present with sweating anomalies. However, comparison with EDA cases harboring mutations in the ectodysplasin pathway identifies some phenotypic differences. Dermatological features (anomalies of hair and sweat glands) are less severe in patients carrying WNT10A mutations and facial dysmorphism can be absent. The dental phenotype consists in microdontia, whereas teeth agenesis is more frequent in patients carrying mutations in the ectodysplasin pathway.
Defects in WNT10A are a cause of odonto-onycho-dermal dysplasia (OODD) [MIM:257980]. OODD is a rare autosomal recessive ectodermal dysplasia in which the presenting phenotype is dry hair, severe hypodontia, smooth tongue with marked reduction of fungiform and filiform papillae, onychodysplasia, keratoderma and hyperhidrosis of palms and soles, and hyperkeratosis of the skin.
Defects in WNT10A are a cause of Schopf-Schulz-Passarge syndrome (SSPS) [MIM:224750]. SSPS is rare ectodermal dysplasia, characterized chiefly by cysts of the eyelid margins, palmoplantar keratoderma, hypodontia, hypotrichosis and nail dystrophy. Multiple eyelid apocrine hidrocystomas are the hallmark of this condition, although they usually appear in adulthood. The concomitant presence of eccrine syringofibroadenoma in most patients and of other adnexal skin tumors in some affected subjects indicates that Schopf-Schulz-Passarge syndrome is a genodermatosis with skin appendage neoplasms.
Sequence similaritiesBelongs to the Wnt family.
Cellular localizationSecreted > extracellular space > extracellular matrix.
- Information by UniProt
- FLJ14301 antibody
- OODD antibody
- Protein Wnt-10a antibody
Lane 1 : Anti-Wnt10a antibody (ab106522) at 1 µg/ml
Lane 2 : Anti-Wnt10a antibody (ab106522) at 2 µg/ml
All lanes : RAW264.7 cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 46 kDa
Paraffin-embedded mouse skeletal muscle tissue stained for Wnt10a using ab106522 at 5 µg/ml in immunohistochemical analysis.
Paraffin-embedded human skeletal muscle tissue stained for Wnt10a using ab106522 at 10 µg/ml in immunohistochemical analysis.
Immunohistochemical analysis of rat bladder tissue, staining Wnt10a with ab106522.
Tissue was fixed with formalin and blocked with 5000 µg/ml BSA for 30 minutes at 22°C; antigen retrieval was by heat mediation in citrate buffer (pH 6). Samples were incubated with primary antibody (1/100 in BSA) for 1 hour at 22°C. An AlexaFluor®555-conjugated goat anti-rabbit polyclonal IgG (1/400) was used as the secondary antibody.
Immunofluorescence of Wnt10a in Human Skeletal Muscle cells using ab106522 at 20 ug/ml.
ab106522 has been referenced in 6 publications.
- Zhong JM et al. Dexmedetomidine Reduces Diabetic Neuropathy Pain in Rats through the Wnt 10a/ß-Catenin Signaling Pathway. Biomed Res Int 2018:9043628 (2018). PubMed: 30622965
- Cheng JM et al. Role of WNT signaling in epididymal sperm maturation. J Assist Reprod Genet 35:229-236 (2018). PubMed: 29152689
- Zhao B et al. Hyperbaric oxygen attenuates neuropathic pain and reverses inflammatory signaling likely via the Kindlin-1/Wnt-10a signaling pathway in the chronic pain injury model in rats. J Headache Pain 18:1 (2017). IHC ; Rat . PubMed: 28058534
- Sun X et al. Colorectal cancer cells suppress CD4+ T cells immunity through canonical Wnt signaling. Oncotarget 8:15168-15181 (2017). PubMed: 28147310
- Chen B et al. Low-magnitude, high-frequency vibration promotes the adhesion and the osteogenic differentiation of bone marrow-derived mesenchymal stem cells cultured on a hydroxyapatite-coated surface: The direct role of Wnt/ß-catenin signaling pathway activation. Int J Mol Med 38:1531-1540 (2016). PubMed: 28026000
- Long A et al. WNT10A promotes an invasive and self-renewing phenotype in esophageal squamous cell carcinoma. Carcinogenesis 36:598-606 (2015). PubMed: 25795715