Overview

  • Product name

  • Description

    Rabbit polyclonal to Wnt10a
  • Host species

    Rabbit
  • Specificity

    Despite the high homology of Wnt10a to Wnt10b, ab106522 will not cross-react with Wnt10b.
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    A 14 amino acid synthetic peptide from near the C terminus of Human Wnt10a (UniProt Q9GZT5).

  • Positive control

    • WB: RAW264.7 cell lysate. IHC-P: Human and mouse skeletal muscle tissue; Rat bladder tissue. ICC/IF: Human skeletal muscle cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab106522 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 46 kDa.
IHC-P Use at an assay dependent concentration.

Target

  • Function

    Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule important in CNS development. Is likely to signal over only few cell diameters.
  • Involvement in disease

    Defects in WNT10A are a cause of ectodermal dysplasia anhidrotic (EDA) [MIM:224900]; also known ectodermal dysplasia hypohidrotic autosomal recessive (HED). Ectodermal dysplasia defines a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. EDA is characterized by sparse hair (atrichosis or hypotrichosis), abnormal or missing teeth and the inability to sweat due to the absence of sweat glands. Note=Most patients carrying WNT10A mutations present with sweating anomalies. However, comparison with EDA cases harboring mutations in the ectodysplasin pathway identifies some phenotypic differences. Dermatological features (anomalies of hair and sweat glands) are less severe in patients carrying WNT10A mutations and facial dysmorphism can be absent. The dental phenotype consists in microdontia, whereas teeth agenesis is more frequent in patients carrying mutations in the ectodysplasin pathway.
    Defects in WNT10A are a cause of odonto-onycho-dermal dysplasia (OODD) [MIM:257980]. OODD is a rare autosomal recessive ectodermal dysplasia in which the presenting phenotype is dry hair, severe hypodontia, smooth tongue with marked reduction of fungiform and filiform papillae, onychodysplasia, keratoderma and hyperhidrosis of palms and soles, and hyperkeratosis of the skin.
    Defects in WNT10A are a cause of Schopf-Schulz-Passarge syndrome (SSPS) [MIM:224750]. SSPS is rare ectodermal dysplasia, characterized chiefly by cysts of the eyelid margins, palmoplantar keratoderma, hypodontia, hypotrichosis and nail dystrophy. Multiple eyelid apocrine hidrocystomas are the hallmark of this condition, although they usually appear in adulthood. The concomitant presence of eccrine syringofibroadenoma in most patients and of other adnexal skin tumors in some affected subjects indicates that Schopf-Schulz-Passarge syndrome is a genodermatosis with skin appendage neoplasms.
  • Sequence similarities

    Belongs to the Wnt family.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links

  • Alternative names

    • FLJ14301 antibody
    • OODD antibody
    • Protein Wnt-10a antibody
    • SSPS antibody
    • STHAG4 antibody
    • Wingless type MMTV integration site family member 10A antibody
    • WN10A_HUMAN antibody
    • wnt10a antibody
    see all

Images

  • Lane 1 : Anti-Wnt10a antibody (ab106522) at 1 µg/ml
    Lane 2 : Anti-Wnt10a antibody (ab106522) at 2 µg/ml

    All lanes : RAW264.7 cell lysate

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 46 kDa

  • Paraffin-embedded mouse skeletal muscle tissue stained for Wnt10a using ab106522 at 5 µg/ml in immunohistochemical analysis.

  • Paraffin-embedded human skeletal muscle tissue stained for Wnt10a using ab106522 at 10 µg/ml in immunohistochemical analysis.

  • Immunohistochemical analysis of rat bladder tissue, staining Wnt10a with ab106522.

    Tissue was fixed with formalin and blocked with 5000 µg/ml BSA for 30 minutes at 22°C; antigen retrieval was by heat mediation in citrate buffer (pH 6). Samples were incubated with primary antibody (1/100 in BSA) for 1 hour at 22°C. An AlexaFluor®555-conjugated goat anti-rabbit polyclonal IgG (1/400) was used as the secondary antibody.

    See Abreview

  • Immunofluorescence of Wnt10a in Human Skeletal Muscle cells using ab106522 at 20 ug/ml.

References

This product has been referenced in:

  • Zhong JM  et al. Dexmedetomidine Reduces Diabetic Neuropathy Pain in Rats through the Wnt 10a/ß-Catenin Signaling Pathway. Biomed Res Int 2018:9043628 (2018). Read more (PubMed: 30622965) »
  • Cheng JM  et al. Role of WNT signaling in epididymal sperm maturation. J Assist Reprod Genet 35:229-236 (2018). Read more (PubMed: 29152689) »
See all 5 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Bladder)
Specification
Bladder
Fixative
10% Buffered Formalin
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 0.01 M Citrate Buffer, PH 6.0
Permeabilization
No
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5000µg/mL · Temperature: 22°C

Abcam user community

Verified customer

Submitted Oct 22 2012

Question
Answer

Thank you for taking time to inform us of the problems you have encountered with the anti-Wnt10a antibody (ab106522). I am sorry to hear this antibody is not providing satisfactory results.

As discussed over the phone,I have arranged for a replacement vial to be sent out to you. Due to the memorial day I am arranging for this to be shipped on the following Tuesday so it should reach you on Wednesday the 30th of May. This is on the order number xxxxx(purchase order number FOCR xxxxx).

I have arranged avial of the lot number GR42482-5 to be sent to you. The lot you have had problems with is GR42482-3 and I think from your order records you have previously been using the lot number GR42482-2 sucessfully. These three lots are actually derived from the samepurification of antibody. The difference is simply that they were aliquotted and put into stock at a different time. This, as well asour records showing nosimilar reports having been made against this antibody,leads me to believe that it was something thatyour GR42482-3vial experienced which has altered the activity. I am sorry for this and the inconvenience this has caused you.

I hope you find the replacement vial to work as expected. However if youdo have any problems please do let me know.

Until then,I wish you all the best with yourreseach.

Read More

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